FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317656
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACT16510.5197446294104314No Hit
TCCATAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA15950.5021154960082606No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT15890.5002266602865993No Hit
CTTATATAGAATGCCAGCCTTCCATAGAGTATGTAAATAATGCCTAGTTT11460.3607676228372831No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11010.3466013549248243No Hit
GTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC10170.320157654821568No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9840.3097690583524315No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG9440.2971768202080238No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8740.27514040345531016No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8730.2748255975017No Hit
CTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA8370.2634925831717329No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8250.2597149117284106No Hit
CCTTTGCAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT7770.24460422595512127No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC7060.22225300324879743No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG6690.21060518296522024No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6610.20808673533633867No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA5650.17786536378976No Hit
CTCTTATATAGAATGCCAGCCTTCCATAGAGTATGTAAATAATGCCTAGT5580.17566172211448863No Hit
GACAAAAACTGCACTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAG5550.17471730425365806No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5510.1734580804392173No Hit
GTATACATGCATATAACGGCATTAAGGCAGGATAGCCACATTGTGTAAAA5490.17282846853199688No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA5410.1703100209031153No Hit
CCCAAGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACT5410.1703100209031153No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT5370.16905079708867454No Hit
GAATACCAGCCTTCCATAGAGTATGTAAATAATGCCTAGTTTTGAAGTAA5260.1655879315989624No Hit
GTCTATAAGCGGGAGGAGTGCGAATCCACACTCCGAAAGATACCAAATAC5250.1652731256453522No Hit
CATTTACAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCT5220.1643287077845216No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5180.16306948397008084No Hit
GCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTT5090.1602362303875891No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4950.15582894703704636No Hit
AGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAAT4940.15551414108343617No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCTACCAATCGGCAGT4930.15519933512982598No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4900.1542549172689954No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCGGACTTCTTCCCTTCTATTCG4770.15016243987206285No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4690.1476439922431813No Hit
GCCAAAGGCAAATCAGGTAGGAGCGGGAGCATTCGGGCCAGGGTTCACCC4670.14701438033596093No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC4570.14386632079985898No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4500.1416626791245876No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4470.140718261263757No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4440.13977384340292642No Hit
CCTGTAAGTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAAC4440.13977384340292642No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCCACTA4380.13788500768126527No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCG4360.13725539577404489No Hit
CTCTATGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCC4240.13347772433072252No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG4210.13253330646989195No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4170.13127408265545118No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT4160.130959276701841No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4160.130959276701841No Hit
CTCCGAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA4100.1290704409801798No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4100.1290704409801798No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG4090.12875563502656961No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4060.12781121716573904No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTTTGACATACT4060.12781121716573904No Hit
CTAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTCTGCG4050.12749641121212885No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCGTTATATGCATGTATA3860.12151509809353515No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3850.12120029213992495No Hit
GTGTTTGTTCCTGTAAGTAGAGGACAAACGGGCAACATACCTTGGTAGTC3760.11836703855743319No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCGTACCTGTC3750.11805223260382301No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA3730.11742262069660261No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3710.11679300878938222No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3660.11521897902133126No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA3630.11427456116050065No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC3610.11364494925328028No Hit
GGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG3590.11301533734605988No Hit
CTTCCATAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG3580.1127005313924497No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT3570.1123857254388395No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3550.11175611353161911No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3530.11112650162439872No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG3440.10829324804190697No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3420.10766363613468657No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA3420.10766363613468657No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTTAGTACAATA3420.10766363613468657No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG3410.1073488301810764No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG3410.1073488301810764No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3380.1064044123202458No Hit
ATACTACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3320.10451557659858463No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3310.10420077064497443No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC3300.10388596469136425No Hit
TATATAGAATGCCAGCCTTCCATAGAGTATGTAAATAATGCCTAGTTTTG3240.10199712896970309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)