FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317656
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA12810.4032664265746594No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT12600.39665550154884527No Hit
GTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC12510.3938222479663535No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11510.3623416526053341No Hit
CTTATATAGAATGCCAGCCTTCCATAGAGTATGTAAATAATGCCTAGTTT10860.34187926562067145No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9540.3003248797441257No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG9170.28867705946054856No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8740.27514040345531016No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8470.2666406427078349No Hit
CCTTTGCAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT7800.24554864381595184No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG7650.2408265545117989No Hit
CTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA7440.23421562948598484No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACT7380.2323267937643237No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC6760.2128088246404916No Hit
GTCTATAAGCGGGAGGAGTGCGAATCCACACTCCGAAAGATACCAAATAC6760.2128088246404916No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6510.20493867580023672No Hit
CTCTTATATAGAATGCCAGCCTTCCATAGAGTATGTAAATAATGCCTAGT6390.2011610043569144No Hit
GCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTT6040.19014279598055758No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT5840.18384667690835368No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA5800.18258745309391292No Hit
GTATACATGCATATAACGGCATTAAGGCAGGATAGCCACATTGTGTAAAA5620.1769209459289294No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTTTGACATACT5460.1718840506711663No Hit
GAATACCAGCCTTCCATAGAGTATGTAAATAATGCCTAGTTTTGAAGTAA5370.16905079708867454No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4990.15708817085148713No Hit
CCCAAGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACT4960.15614375299065655No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4870.15331049940816482No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4840.15236608154733422No Hit
CCTGTAAGTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAAC4780.15047724582567304No Hit
GACAAAAACTGCACTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAG4740.14921802201123227No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCG4730.14890321605762208No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4700.1479587981967915No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4700.1479587981967915No Hit
CTCCGAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA4660.1466995743823507No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT4660.1466995743823507No Hit
GTGTTTGTTCCTGTAAGTAGAGGACAAACGGGCAACATACCTTGGTAGTC4620.14544035056790994No Hit
AGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAAT4490.1413478731709774No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT4330.13631097791321428No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4320.1359961719596041No Hit
GCCAAAGGCAAATCAGGTAGGAGCGGGAGCATTCGGGCCAGGGTTCACCC4230.13316291837711236No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCGTTATATGCATGTATA4210.13253330646989195No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4210.13253330646989195No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG4190.13190369456267156No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4180.13158888860906137No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4170.13127408265545118No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4150.1306444707482308No Hit
CATTTACAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCT4150.1306444707482308No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4140.1303296647946206No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4120.12970005288740022No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4100.1290704409801798No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTTAGTACAATA4020.12655199335129824No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG4000.12592238144407789No Hit
GGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG3940.12403354572241672No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCTACCAATCGGCAGT3930.1237187397688065No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3930.1237187397688065No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCGGACTTCTTCCCTTCTATTCG3910.12308912786158611No Hit
CTCTATGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCC3730.11742262069660261No Hit
CTAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTCTGCG3710.11679300878938222No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3640.11458936711411087No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCGTACCTGTC3570.1123857254388395No Hit
CTTCCATAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG3500.11018208376356814No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3460.10892285994912734No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA3450.10860805399551716No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3440.10829324804190697No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3440.10829324804190697No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3410.1073488301810764No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG3410.1073488301810764No Hit
CTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA3330.10483038255219483No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC3300.10388596469136425No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG3260.10262674087692346No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCCACTA3250.10231193492331327No Hit
ATACTACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3190.1004230992016521No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3180.1001082932480419No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)