FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537388
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC30480.5671879535828861No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA29750.5536037276604613No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG26180.48717128034120605No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT25180.46856275168035016No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA24890.46316627836870194No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT23020.4283683297729015No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23010.428182244486293No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG20840.38780173729223577No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG20600.38333569041363036No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC18790.3496542535374813No Hit
ATGTAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC18440.34314126850618176No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA18090.3366282834748822No Hit
CTTGATTTTCATTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAA18010.33513960118201375No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT17630.3280683602908885No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA16280.3029468465987331No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC15200.28284963564500887No Hit
GAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTATTGATT14930.27782533290657774No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC14510.2700097508690183No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG13980.2601472306787647No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG13960.2597750601055476No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA13590.2528899045010309No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC13560.2523316486412052No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG12760.23744482571252057No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC12250.22795447609548408No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA12240.2277683908088755No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA12000.22330234393027013No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT11880.22106932049096745No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG11850.22051106463114173No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT11730.21827804119183905No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG11720.21809195590523048No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG11680.21734761475879624No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT11340.21102071501410527No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA11280.20990420329445392No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA11080.20618249756228277No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA11010.20487990055602284No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT11000.20469381526941427No Hit
TTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC10790.20078602425063455No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC10570.19669214794524625No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA10550.19631997737202916No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG10510.1955756362255949No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT10410.19371478335950934No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG10180.18943482176751247No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC10110.18813222476125258No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT10080.1875739689014269No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT10070.18738788361481834No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC10050.18701571304160122No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG9900.18422443374247285No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT9890.18403834845586428No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTAATGAAA9760.18161923972995303No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG9660.17975838686386744No Hit
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9530.1773392781379562No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG9490.17659493699152196No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG9430.1754784252718706No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC9350.17398974297900213No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA9300.17305931654595935No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT9270.17250106068613366No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9200.17119846367987376No Hit
GTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA8910.16580199036822557No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG8750.16282462578248863No Hit
TTATGAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA8620.16040551705657738No Hit
GGTCTTGCATGGCCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC8590.1598472611967517No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT8450.15724206718423187No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA8430.15686989661101478No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG8420.1566838113244062No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC8320.15482295845832061No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCGGACC8290.15426470259849495No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT8250.15352036145206072No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATTACAATA8200.1525899350190179No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC8200.1525899350190179No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG8040.14961257043328097No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATTACAATATGTT7780.14477435298145847No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG7760.14440218240824135No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC7700.14328567068859No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTG7670.1427274148287643No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7650.14235524425554721No Hit
GCACGGGGCCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7610.14161090310911298No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT7600.14142481782250443No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG7500.13956396495641882No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC7430.13826136795015892No Hit
GCGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGAC7430.13826136795015892No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA7420.13807528266355035No Hit
ATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAA7370.13714485623050757No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC7350.13677268565729045No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA7330.13640051508407333No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG7230.13453966221798774No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG7230.13453966221798774No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG7210.13416749164477063No Hit
ATACTACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7120.1324927240652936No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTAAT6990.13007361533938236No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA6960.12951535947955667No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCT6960.12951535947955667No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG6870.12784059190007965No Hit
ATCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT6810.1267240801804283No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATTACAAT6810.1267240801804283No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG6780.1261658243206026No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC6740.12542148317416837No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTCATTACAATA6590.12263020387504No Hit
GCCCAGGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTT6550.12188586272860577No Hit
ATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCGGACCGTTGC6430.11965283928930306No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATT6370.11853632756965173No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA6270.11667547470356612No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC6270.11667547470356612No Hit
GGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTAC6260.11648938941695758No Hit
ATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGT6230.11593113355713192No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT6190.11518679241069767No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT6180.11500070712408912No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6180.11500070712408912No Hit
CCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATGGA5980.11127900139191794No Hit
ACCCTGCACCGAACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTG5980.11127900139191794No Hit
GAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCTTGCGAAAG5940.11053466024548372No Hit
ATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGC5910.10997640438565803No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTCATTACAATATGTT5870.10923206323922381No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT5860.10904597795261524No Hit
GCCTCACCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAA5770.10737121037313821No Hit
AGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCC5770.10737121037313821No Hit
TCCTTGAAGTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAA5760.10718512508652966No Hit
GATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC5740.10681295451331255No Hit
ACCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCAC5710.10625469865348687No Hit
GTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTC5710.10625469865348687No Hit
GCTTGATTTTCATTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCA5680.1056964427936612No Hit
GGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5670.10551035750705264No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT5620.10457993107400984No Hit
GGTTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCATCCAGTGGGGG5600.10420776050079272No Hit
CTGATGGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACA5590.10402167521418416No Hit
GCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTT5520.10271907820792427No Hit
TGCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACA5520.10271907820792427No Hit
TGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTC5520.10271907820792427No Hit
ATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACC5490.10216082234809859No Hit
GCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAG5470.10178865177488147No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG5470.10178865177488147No Hit
CTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTA5450.10141648120166435No Hit
CAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCC5440.10123039591505578No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC5430.10104431062844724No Hit
GGGCCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCC5420.10085822534183868No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA5390.10029996948201299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)