FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537388
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA32840.6111040812225059No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC30910.5751896209070542No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT27630.514153646899447No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA26510.4933120947992884No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG24930.4639106195151362No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT24150.44939596715966856No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG23720.4413942998355006No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22940.42687964748003304No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG20820.3874295667190187No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC18800.3498403388240898No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT18600.3461186330919187No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA18250.3396056480606191No Hit
ATGTAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC17570.32695184857123716No Hit
CTTGATTTTCATTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAA17510.32583533685158583No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC17230.3206249488265462No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA15850.29494517927456515No Hit
GAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTATTGATT15360.28582700023074575No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG14420.26833498328954125No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG13650.25400641622068226No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC13540.25195947806798813No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA13370.24879602819564262No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA13340.24823777233581695No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA13290.24730734590277417No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC13250.24656300475633994No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG13180.24526040775008No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA12760.23744482571252057No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG12650.2353978875598264No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT12380.23037358482139533No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG12360.23000141424817824No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA12260.22814056138209265No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC12160.22627970851600704No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT11950.22237191749722732No Hit
TTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC11750.21865021176505614No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC11410.21232331202036517No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG11390.21195114144714808No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA11210.20860160628819402No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC11140.2072990092819341No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT10950.20376338883637146No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA10870.20227470654350302No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT10870.20227470654350302No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT10840.20171645068367736No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC10800.20097210953724312No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG10620.19762257437828906No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTAATGAAA10310.19185393049342375No Hit
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC10260.19092350406038097No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC10150.1888765659076868No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT10130.1885043953344697No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT10040.18682962775499265No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG9860.18348009259603862No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG9830.18292183673621296No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT9720.1808748985835188No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT9670.17994447215047601No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG9660.17975838686386744No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATTACAATA9460.1760366811316963No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA9410.17510625469865349No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG9330.17361757240578501No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG9270.17250106068613366No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9240.171942804826308No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCGGACC9190.17101237839326522No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT8890.16542981979500845No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT8820.16412722278874853No Hit
TTATGAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA8770.16319679635570575No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC8730.1624524552092715No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA8710.1620802846360544No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG8590.1598472611967517No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG8300.1544507878851035No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG8290.15426470259849495No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG8230.1531481908788436No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG8190.15240384973240934No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC8190.15240384973240934No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATTACAATATGTT8180.1522177644458008No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA8120.15110125272614944No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTG7930.14756563228058683No Hit
GTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA7920.1473795469939783No Hit
GGTCTTGCATGGCCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC7790.14496043826806704No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7790.14496043826806704No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG7790.14496043826806704No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTAAT7780.14477435298145847No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC7720.1436578412618071No Hit
GCACGGGGCCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7650.14235524425554721No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT7540.14030830610285305No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTCATTACAATA7540.14030830610285305No Hit
ATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAA7450.13863353852337604No Hit
ATACTACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7360.136958770943899No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG7320.1362144297974648No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG7180.13360923578494496No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA7150.1330509799251193No Hit
GTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTC7150.1330509799251193No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG7130.13267880935190218No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG7090.13193446820546792No Hit
ACCCTGCACCGAACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTG7030.13081795648581657No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATTACAAT6970.12970144476616524No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA6950.1293292741929481No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCT6950.1293292741929481No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC6930.12895710361973098No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC6920.12877101833312243No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC6890.12821276247329677No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATT6880.1280266771866882No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT6820.12691016546703687No Hit
CCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATGGA6710.12486322731434271No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT6690.1244910567411256No Hit
ATCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT6680.12430497145451704No Hit
GCCTCACCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAA6640.1235606303080828No Hit
ATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGT6610.12300237444825712No Hit
GCGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGAC6580.12244411858843143No Hit
GCCCAGGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTT6560.12207194801521434No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6450.1200250098625202No Hit
ATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGC6410.11928066871608595No Hit
GAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCTTGCGAAAG6360.11835024228304317No Hit
GGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTAC6290.11704764527678325No Hit
ATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACC6190.11518679241069767No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT6190.11518679241069767No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA6150.11444245126426343No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG6110.11369811011782921No Hit
CTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT6060.11276768368478642No Hit
ACCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCAC6050.11258159839817786No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT6000.11165117196513506No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTCATTACAATATGTT6000.11165117196513506No Hit
ATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCGGACCGTTGC5870.10923206323922381No Hit
ACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCGAGGACT5810.10811555151957244No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC5760.10718512508652966No Hit
GCCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5720.10644078394009542No Hit
CATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGG5690.10588252808026975No Hit
GCTTGATTTTCATTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCA5620.10457993107400984No Hit
GATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC5560.10346341935435847No Hit
CTTTACCACAGGAACATATTGTAATGAAACTCAAGCAATGTTTTCGGAAA5560.10346341935435847No Hit
GCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTC5540.10309124878114138No Hit
TGCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACA5500.10234690763470713No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC5390.10029996948201299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)