FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320047
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT13660.4268123119416835No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12290.38400609910419403No Hit
TCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGG10350.32339000209344254No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9420.29433177002127814No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGGCATACT9280.2899574125050383No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC9060.28308342212237575No Hit
CTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8680.27121016600686776No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8530.2665233543823251No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8430.26339881329929665No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT8290.25902445578305683No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7560.23621530587694933No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT7110.2221548710033214No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT7010.21903032992029295No Hit
GTTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA6960.21746805937877875No Hit
CTTTATACCTCTATTACCAATTTTCTTGTGTCTTTGGGTATACATTTGAA6750.21090652310441904No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6710.20965670667120764No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6570.20528234915496787No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6190.19340909303945983No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6130.19153436838964277No Hit
GTTGTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA6070.18965964373982572No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC6040.1887222814149172No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6030.18840982730661432No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6000.1874724649817058No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5880.1837230156820717No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5880.1837230156820717No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5790.1809109287073461No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA5790.1809109287073461No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5690.17778638762431767No Hit
GTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT5510.1721622136748665No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5250.16403840685899257No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5000.15622705415142152No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4910.15341496717669592No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4890.15279005896009024No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA4760.1487281555521533No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4670.1459160685774277No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCATGT4640.14497870625251916No Hit
TGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCG4510.1409168028445822No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA4500.14060434873627936No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4450.13904207819476513No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG4440.1387296240864623No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4420.1381047158698566No Hit
ATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGA4390.13716735354494808No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4390.13716735354494808No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT4390.13716735354494808No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4360.13622999122003954No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4290.13404281246191965No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4260.13310545013701114No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4170.13029336316228554No Hit
GGAGCACAACTTATCGGGACTGACAACTCTGTTGTCCTCTCTCGGAAATA4070.12716882207925712No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4020.1256065515377429No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG3970.12404428099622868No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAAGGGGCAGCA3890.12154464812980594No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3860.1206072858048974No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT3850.12029483169659456No Hit
CTCTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT3840.11998237758829172No Hit
GTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC3740.11685783650526328No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG3720.11623292828865761No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTC3720.11623292828865761No Hit
CTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC3720.11623292828865761No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3720.11623292828865761No Hit
GTTTTTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA3670.11467065774714338No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACATG3670.11467065774714338No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3670.11467065774714338No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG3660.11435820363884056No Hit
CTATAGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAG3610.11279593309732633No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA3600.1124834789890235No Hit
GACAGAAACTGCACTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAG3590.11217102488072066No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT3540.11060875433920644No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3540.11060875433920644No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG3530.11029630023090357No Hit
CCTCCAATTTGTCCTGGCTATCGCTGGATGTGTCTGCGGCGTTTTATCAT3520.10998384612260073No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC3510.1096713920142979No Hit
ATTTACATACTCTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACA3510.1096713920142979No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTTGGCCGGAACGGCAGATGAAGAAGG3480.10873402968938937No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA3480.10873402968938937No Hit
GTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA3460.10810912147278368No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG3450.10779666736448085No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3420.1068593050395723No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA3400.10623439682296662No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA3390.10592194271466379No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA3370.1052970344980581No Hit
TATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTG3360.10498458038975525No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG3350.10467212628145241No Hit
GAGCAAAACTTATCGGGACTGACAACTCTGTTGTCCTCTCTCGGAAATAC3300.1031098557399382No Hit
TATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTC3300.1031098557399382No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC3290.10279740163163535No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC3270.10217249341502967No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3240.10123513109012114No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3240.10123513109012114No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATA3220.10061022287351545No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG3210.10029776876521261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)