FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457579
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19720.430963833567537No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG19600.4283413355945094No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA15140.33087182759698325No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14410.3149182982610653No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT13570.29656081244987204No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12370.27033583271959594No Hit
GGTTAGGATAGAACCTAGCCGGCATAATTAATTTTAATCTCCTTTTTTCA11870.2594087578319809No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11680.2552564693746872No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA11670.2550379278769349No Hit
CTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG11550.2524154299039073No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCGGCTAGG11240.24564064347358597No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATCCTTTGACATACT10220.2233494107028513No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10110.220945454227576No Hit
CTATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATA10080.2202898297343191No Hit
CTCCAAAAGACACCAAATACTCAAGTACAGTTTCTCTTCCAAAAGTAAGA9220.20149526092762124No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCCTCCTGT9190.20083963643436434No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8720.1905681860400062No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8570.18729006357372172No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8330.18204506762766648No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8090.17680007168161127No Hit
ATGTTAATATGGGCCTAAAAATAAGACAACTCTTGTGGTTTCACATTTCC8030.17548882269509744No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7990.17461465670408827No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7770.16980674375353763No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7710.16849549476702383No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7660.16740278727826233No Hit
GGATAGAACCTAGCCGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7550.16499883080298702No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT7340.16040945935018872No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7330.1601909178524364No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7250.158442585870418No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7190.15713133688390418No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6840.14948238446257367No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6810.14882675996931677No Hit
ATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATATT6750.14751551098280297No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6740.14729696948505067No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6540.14292613953000466No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6340.13855530957495865No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACC6190.13527718710867415No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6070.13265468913564651No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6020.13156198164688504No Hit
TCCCGATACAGAGCAGAAGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5970.1304692741581235No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5870.1282838591806005No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCGGGATGCTGGATCTTCC5860.1280653176828482No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACA5860.1280653176828482No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG5820.127191151691839No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5740.1254428197098206No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5710.12478719521656369No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5690.1243501122210591No Hit
CTCAAGTACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5690.1243501122210591No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5670.1239130292255545No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5660.1236944877278022No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGTA5560.12150907275027918No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTCTATTCTTCTTCTGTACCTATT5500.12019782376376537No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5490.1199792822660131No Hit
ATACAGCACTCAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGAATCTG5460.11932365777275618No Hit
CTATTCTTCTTCTGTACCTATTTTTAATCCTGAGTGGCAAACTCCCTCCT5420.11844949178174698No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5390.11779386728849008No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5260.11495282781771017No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTCTATTC5260.11495282781771017No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5250.11473428631995786No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAAGCGGTGTCGAGGAGATCTCGAA5250.11473428631995786No Hit
GTTAATATGGGCCTAAAAATAAGACAACTCTTGTGGTTTCACATTTCCTG5220.11407866182670096No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC5200.11364157883119637No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5190.11342303733344405No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACAACCGACCT5170.11298595433793947No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5090.11123762235592106No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5070.11080053936041645No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC5030.10992637336940726No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5010.10948929037390265No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4960.10839658288514115No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4930.10774095839188425No Hit
AAATAAGACAACTCTTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGA4920.10752241689413193No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4810.10511846041885663No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATA4760.10402575293009514No Hit
TCACCATACAGCACTCAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGA4760.10402575293009514No Hit
GTACAATATGCTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC4730.10337012843683822No Hit
ATATGCTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA4710.10293304544133364No Hit
GGATTAAAAATAGGTACAGAAGAAGAATAGAGCCCAGTAAAGTTTCCCAC4700.10271450394358132No Hit
AGTTAAGGGAGTAACCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4690.10249596244582902No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4660.10184033795257212No Hit
GAATACAAGTGCAGTTTCCATCCGAAGGTTTTGTACAGCAACAAGAGGGA4650.10162179645481983No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCCGG4650.10162179645481983No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAAT4650.10162179645481983No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC4630.1011847134593152No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4620.10096617196156292No Hit
GTTTTAGTACAATATGCTCCTGTGGTAAGGTACCCCAACTTCCAATTACA4610.10074763046381062No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTCTATTCTTCTTCTG4590.10031054746830602No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4580.10009200597055372No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)