FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457579
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG20360.44495048942368426No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19630.4289969600877663No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13960.30508393086221175No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA13530.29568664645886283No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12950.28301123958922936No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT12660.2766735361544127No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCGGCTAGG12200.2666206272578069No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA11810.2580975088454671No Hit
CTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG11300.24695189246009977No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATCCTTTGACATACT11290.2467333509623475No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10960.23952148153652156No Hit
GGTTAGGATAGAACCTAGCCGGCATAATTAATTTTAATCTCCTTTTTTCA10880.23777314955450318No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10320.2255348256803743No Hit
CTATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATA10050.2196342052410622No Hit
CTCCAAAAGACACCAAATACTCAAGTACAGTTTCTCTTCCAAAAGTAAGA9650.21089254533097015No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCCTCCTGT9300.20324359290963967No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8390.18335631661418028No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8380.18313777511642798No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8250.18029673564564808No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8090.17680007168161127No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8020.17527028119734517No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7760.16958820225578536No Hit
ATGTTAATATGGGCCTAAAAATAAGACAACTCTTGTGGTTTCACATTTCC7560.16521737230073932No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7420.16215779133220712No Hit
ATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATATT7330.1601909178524364No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT7070.15450883891087658No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7060.1542902974131243No Hit
GGATAGAACCTAGCCGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7020.15341613142211508No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6930.15144925794234437No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6910.15101217494683977No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6740.14729696948505067No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6720.14685988648954607No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACAACCGACCT6290.13746260208619712No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6210.13571427010417872No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6200.13549572860642642No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACC6180.13505864561092182No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6160.13462156261541725No Hit
CTATTCTTCTTCTGTACCTATTTTTAATCCTGAGTGGCAAACTCCCTCCT6110.13352885512665572No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCGGGATGCTGGATCTTCC5960.1302507326603712No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5920.129376566669362No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5910.1291580251716097No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5900.1289394836738574No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5870.1282838591806005No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG5860.1280653176828482No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5700.1245686537188114No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5570.12172761424803148No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5540.12107198975477458No Hit
ATACAGCACTCAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGAATCTG5540.12107198975477458No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5540.12107198975477458No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5530.12085344825702228No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGTA5500.12019782376376537No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5500.12019782376376537No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTCTATTCTTCTTCTGTACCTATT5490.1199792822660131No Hit
AAATAAGACAACTCTTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGA5460.11932365777275618No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5400.11801240878624238No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTCTATTCTTCTTCTG5400.11801240878624238No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5390.11779386728849008No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5350.11691970129748087No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5330.11648261830197626No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTCTATTC5250.11473428631995786No Hit
CTCAAGTACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5210.11386012032894866No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5180.11320449583569175No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAAGCGGTGTCGAGGAGATCTCGAA5160.11276741284018715No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG5140.11233032984468257No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5130.11211178834693025No Hit
GTACAATATGCTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC5120.11189324684917797No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5080.11101908085816875No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGATGGAAACTGC5030.10992637336940726No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATA5000.10927074887615035No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACA5000.10927074887615035No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4910.10730387539637964No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4860.10621116790761814No Hit
GGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCG4840.10577408491211353No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC4830.10555554341436124No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4820.10533700191660894No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4800.10489991892110434No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4770.10424429442784744No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4720.10315158693908592No Hit
ATATGCTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA4710.10293304544133364No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4690.10249596244582902No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4630.1011847134593152No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4610.10074763046381062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)