FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724727
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40500.5588311184763366No Hit
GTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAA21610.2981812461795959No Hit
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC20840.2875565557789347No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC20600.2842449639657416No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT19820.273482290572864No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA19620.2707226307285364No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA19280.2660312089931795No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCCGG19180.2646513790710157No Hit
GGATCATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA17120.23622688267444153No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA16550.22836185211810792No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT16440.22684403920372775No Hit
ATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA16410.2264300902270786No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG16080.2218766514839381No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA15540.2144255699042536No Hit
CCTCCCGCATACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15460.21332170596652256No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC14740.20338693052694323No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14290.19717769587720618No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT14050.19386610406401308No Hit
CATGTATACAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC13680.18876073335200705No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATA13440.18544914153881392No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG13350.1842072946088665No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA12280.16944311444171392No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11900.16419976073749148No Hit
CTTTTACACAATGTGGCTATCCTGCTCTGATGCCTTTATATGCATGTATA11840.16337186278419322No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG11720.16171606687759668No Hit
GTGGAATTACTCTCTTTTTTGCCTACTGACTTCTTTCCTTCCATTCGAGA11130.1535750703368303No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG11060.15260918939131562No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC11050.15247120639909925No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT11040.15233322340688285No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG11040.15233322340688285No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG10780.148745665609257No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10770.14860768261704063No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG10600.14626197174936217No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG10440.1440542438739001No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT10290.1419844989906544No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACAACCGACCT10230.14115660103735614No Hit
CATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA10190.14060466906849062No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCCGGATAATACGGTTTAATGCC9930.13701711127086474No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA9860.1360512303253501No Hit
CATTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9760.13467140040318631No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT9750.1345334174109699No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA9640.13301560449658975No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA9580.13218770654329146No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC9480.13080787662112767No Hit
CTTTACCACAGGATCATATTATACAAAAACTCAAGCAATGTTTTCGAAAA9440.13025594465226217No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG9360.12915208071453113No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9330.12873813173788198No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTATAAT9310.12846216575344924No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC9300.12832418276123286No Hit
CTATATAAGAGAGAAACCACACGCAGCGCCTCATTTTGTGGGTCACCATA9210.12708233583128545No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC9090.12542653992468888No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAATATTCATTTACAGG9060.12501259094803974No Hit
GCCATGGGAAGGAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTC9000.12418469299474147No Hit
GTATGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA8970.12377074401809234No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTATAATA8950.12349477803365957No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG8790.12128705015819749No Hit
CCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA8760.12087310118154837No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8750.12073511818933198No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC8710.12018318622046646No Hit
ATATAAGAGAGAAACCACACGCAGCGCCTCATTTTGTGGGTCACCATATT8620.11894133929051905No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCG8620.11894133929051905No Hit
GATTGTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGT8460.11673361141505698No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATGGAAGGAAA8460.11673361141505698No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG8320.11480184952402768No Hit
ATCCTGCTCTGATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTT8300.11452588353959492No Hit
CCCGCATACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC8260.1139739515707294No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCTGGTC8220.11342201960186388No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA8200.11314605361743113No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8190.11300807062521473No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG8140.11231815566413283No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8100.11176622369526731No Hit
GAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCC8090.11162824070305094No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTT8070.1113522747186182No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTTTTGCCTACTG8040.11093832574196905No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8000.11038639377310352No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA7950.10969647881202164No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT7940.10955849581980526No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATAC7940.10955849581980526No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGT7800.10762673392877595No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7750.10693681896769404No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT7690.10610892101439577No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7650.10555698904553025No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACAT7580.10459110810001558No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7520.10376321014671731No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7500.10348724416228455No Hit
GTCTTTGGGTATACATTTGAATCCTAATAAAACCAAACGTTGGGGCTACT7420.10238338022455352No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAAT7380.101831448255688No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCATACAGACCACCAAATGCC7360.10155548227125524No Hit
GTTGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG7340.1012795162868225No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG7310.10086556731017335No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACA7300.10072758431795698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)