FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724727
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40270.5556575096553599No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCCGG22380.3088059365802571No Hit
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC21960.30301065090716917No Hit
GTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAA21180.2922479775142916No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC20740.2861767258567709No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA20200.2787256442770864No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT20160.27817371230822086No Hit
ATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA17950.24767947102840104No Hit
GGATCATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA16870.23277730786903209No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT16540.22822386912589154No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG16020.22104875353063982No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA15780.2177371617174467No Hit
CCTCCCGCATACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14930.20600860737905444No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA14280.19703971288498978No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT13950.1924862741418493No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13520.18655300547654496No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG13430.18531115854659755No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC13050.18006780484237514No Hit
CATGTATACAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC12430.1715128593249596No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA12400.17109891034831046No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATA12280.16944311444171392No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACAACCGACCT12170.16792530152733373No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11710.16157808388538028No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG11570.15964632199435097No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG11270.1555068322278596No Hit
CTTTTACACAATGTGGCTATCCTGCTCTGATGCCTTTATATGCATGTATA11090.15302313836796477No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG10960.15122935946915184No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA10860.14984952954698805No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG10830.1494355805703389No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA10790.14888364860147338No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10730.1480557506481751No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG10570.14584802277271303No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA10460.14433020985833286No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCCGGATAATACGGTTTAATGCC10450.1441922268661165No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC10350.14281239694395267No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT10030.13839694119302856No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTATAATA9880.13632719630978285No Hit
GTATGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA9850.1359132473331337No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9740.13439543441875354No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG9700.13384350244988805No Hit
CATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA9590.13232568953550786No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATGGAAGGAAA9510.13122182559777681No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT9490.13094585961334407No Hit
GTGGAATTACTCTCTTTTTTGCCTACTGACTTCTTTCCTTCCATTCGAGA9410.12984199567561303No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG9270.12791023378458372No Hit
CTTTACCACAGGATCATATTATACAAAAACTCAAGCAATGTTTTCGAAAA9250.12763426780015094No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC9220.12722031882350182No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC9210.12708233583128545No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC9200.12694435283906907No Hit
CATTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9090.12542653992468888No Hit
CTATATAAGAGAGAAACCACACGCAGCGCCTCATTTTGTGGGTCACCATA9040.12473662496360698No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCG8970.12377074401809234No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT8950.12349477803365957No Hit
GCCATGGGAAGGAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTC8890.12266688008036128No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTATAAT8870.12239091409592853No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAATATTCATTTACAGG8800.12142503315041389No Hit
GTCTTTGGGTATACATTTGAATCCTAATAAAACCAAACGTTGGGGCTACT8730.12045915220489922No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8700.1200452032282501No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG8670.11963125425160095No Hit
ATATAAGAGAGAAACCACACGCAGCGCCTCATTTTGTGGGTCACCATATT8370.11549176448510956No Hit
ATCCTGCTCTGATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTT8350.1152157985006768No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCTGGTC8340.11507781550846043No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGT8320.11480184952402768No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC8030.11080034274975267No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTTTTGCCTACTG8000.11038639377310352No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG7990.11024841078088715No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCATACAGACCACCAAATGCC7940.10955849581980526No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7870.10859261487429059No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACAT7780.10735076794434319No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC7750.10693681896769404No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT7660.10569497203774662No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG7630.10528102306109749No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACA7600.10486707408444834No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA7600.10486707408444834No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7580.10459110810001558No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7580.10459110810001558No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7530.1039011931389337No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTT7470.10307329518563543No Hit
GATTGTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGT7460.10293531219341903No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7410.10224539723233714No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG7410.10224539723233714No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7370.10169346526347162No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG7360.10155548227125524No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG7320.10100355030238974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)