FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633113
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA37200.5875728345492827No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG35510.5608793374958341No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35350.5583521425085254No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT34200.5401879285372437No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT31310.4945404690789796No Hit
CATATATAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA30920.4883804312974145No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT30470.4812726953956087No Hit
GTATACATGCATATATAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA28800.4548950977155737No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA28600.45173610398143776No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC27570.43546728625063774No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC25130.3969275626941794No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT24330.38429158775763567No Hit
TCCTAGAAGTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAA23250.3672330215933017No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGT22360.3531754994763968No Hit
TGCATATATAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAG21920.34622571326129775No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG21610.34132927297338705No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG21310.33659078237218315No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC21090.33311588926463365No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATATGTT21030.33216819114439283No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA20980.33137844271085887No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATA20580.32506045524258703No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAAT20540.32442865649575986No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC20450.32300710931539867No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG20030.31637322247371324No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA19390.3062644425244782No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG19220.30357929785046267No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC18410.2907853732272122No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG18380.29031152416709183No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA18270.28857407761331705No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA18210.2876263794930763No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTATATATGCATGTATA16830.2658293227275384No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC16020.2530353981042878No Hit
CTTTACCACAGGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAA15680.24766510875625677No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCACCAACTTACAAGGCC15510.24497996408224124No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA15430.24371636658858686No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT14800.2337655363260587No Hit
GCTTAGATTGTATACATGCATATATAGGCATCAAAGCAGGATAGCCACAT14720.23250193883240433No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA14680.23187014008557716No Hit
CTATATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCACCAACT14680.23187014008557716No Hit
ATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA14290.22571010230401206No Hit
GCTTTAGTTTTTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCA14020.22144546076292856No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA13720.21670697016172466No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA13400.2116525801871072No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT13280.20975718394662562No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTT13250.20928333488650525No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG13160.20786178770614408No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACA13010.20549254240554213No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC12940.20438689459859458No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT12940.20438689459859458No Hit
ATATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCACCAACTTA12840.20280739773152662No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG12790.20201764929799262No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT12760.20154380023787222No Hit
GATTGTATACATGCATATATAGGCATCAAAGCAGGATAGCCACATTGTGT12250.1934883662158256No Hit
GCGTTCTATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGT12210.19285656746899843No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT12010.1896975737348625No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG11820.18669652968743336No Hit
AACTAAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTATTGATTGG11750.1855908818804858No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG11690.184643183760245No Hit
GGGTAAAGGTGCAAATATTGGTTACACAGAAAGGCCTTGTAAGTTGGTGA11540.18227393845964307No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA11530.18211598877293625No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT11450.1808523912792819No Hit
ATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTG11070.17485030318442366No Hit
TCCAACGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGATTG10910.17232310819711488No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG10340.16331997605482748No Hit
GTTTTTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA10240.16174047918775955No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC10040.1585814854536236No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC9690.15305324641888574No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG9560.15099990049169737No Hit
GGGTAAAGGTGCAAATATTGGTTACGCAGAAAGGCCTTGTAAGTTGGTGA9470.1495783533113362No Hit
CATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGG9470.1495783533113362No Hit
GTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT9470.1495783533113362No Hit
ATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTT9470.1495783533113362No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTC9350.14768295707085463No Hit
CAGGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGT9300.14689320863732067No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCAC9230.1457875608303731No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT9160.14468191302342553No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC9140.14436601365001192No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT9110.14389216458989154No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA9100.14373421490318475No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATA9010.14231266772282358No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG8990.14199676834940997No Hit
GTTTTATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTG8760.13836392555515367No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA8760.13836392555515367No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC8720.13773212680832647No Hit
ATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGT8700.13741622743491289No Hit
TCCTGGAAGTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAA8560.13520493182101773No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTGCAAATATTGGTTACGC8500.13425723370077697No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT8460.13362543495394977No Hit
CATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAA8390.1325197871470022No Hit
CTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTA8310.13125618965334782No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT8300.13109823996664102No Hit
CATGCATATATAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGC8250.13030849153310703No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG8010.1265176990521439No Hit
GTGAAAGCCTGCTTAGATTGTATACATGCATATATAGGCATCAAAGCAGG8000.12635974936543712No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7900.12478025249836916No Hit
CCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTT7820.1235166550047148No Hit
GTACTTTACCACAGGAACATATTGTACAAAAACTAAAGCAATGTTTTCGG7730.12209510782435362No Hit
GATAAAACGCCGCAGACACATCCAACGATAGCCAGGACAAATTGGAGGAC7680.12130535939081964No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7660.12098946001740606No Hit
CAGAAAGGCCTTGTAAGTTGGTGAGAAAGTGAAAGCCTGCTTAGATTGTA7600.12004176189716528No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC7600.12004176189716528No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7580.11972586252375168No Hit
CTTCCAGGAACATCAACTACCAGCACGGGACCATGCAAGACCTGCACGAT7580.11972586252375168No Hit
AAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCC7510.11862021471680412No Hit
TGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTC7510.11862021471680412No Hit
GGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAA7500.11846226503009732No Hit
TATATAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAA7480.11814636565668371No Hit
AATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAAT7450.11767251659656333No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG7390.11672481847632256No Hit
TTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGG7270.11482942223584099No Hit
GTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCA7210.11388172411560021No Hit
TTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTA7210.11388172411560021No Hit
GGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATT7130.11261812662194585No Hit
CGCATGCGCTGATGGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTC7110.11230222724853225No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA6800.10740578696062156No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6800.10740578696062156No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG6780.10708988758720797No Hit
TTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACC6770.10693193790050118No Hit
GGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTAC6750.10661603852708758No Hit
CGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGG6720.1061421894669672No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA6710.10598423978026039No Hit
GCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTT6690.1056683404068468No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA6620.10456269259989924No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG6570.10377294416636525No Hit
GTGCAAATATTGGTTACACAGAAAGGCCTTGTAAGTTGGTGAGAAAGTGA6370.10061395043222932No Hit
GTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTAG6360.10045600074552252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)