FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633113
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG36990.58425589112844No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT35850.5662496268438652No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35230.5564567462680438No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA35110.5545613500275622No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT33260.5253406579868048No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT29730.4695844185793057No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA27450.43357189001015617No Hit
GTATACATGCATATATAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA26980.4261482547349368No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC25750.40672044327000073No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG25700.40593069483646677No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC24550.38776648086518517No Hit
CATATATAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA23850.3767100027957095No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC22980.3629683800522182No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT22610.3571242416440667No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATA22220.3509642038625016No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG20470.32332300868881225No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAAT20240.31969016589455596No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA19840.31337217842628406No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA19820.3130562790528705No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG19690.3110029331256821No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC19640.31021318469214815No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC19360.3057905934643579No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGT19290.30468494565741033No Hit
TCCTAGAAGTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAA19140.3023157003568084No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA18800.29694541100877725No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA18630.2942602663347617No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATATGTT18480.29189102103415976No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG17860.28209814045833836No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTATATATGCATGTATA17640.27862324735078886No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG17490.2762540020501869No Hit
TGCATATATAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAG17430.27530630392994615No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC17240.272305259882517No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT16070.25382514653782184No Hit
CTTTACCACAGGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAA15880.2508241024903927No Hit
ATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA15620.24671741063601602No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT15230.24055737285445092No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACA14640.23123834133874996No Hit
GCTTAGATTGTATACATGCATATATAGGCATCAAAGCAGGATAGCCACAT14580.2302906432185092No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT14340.22649985073754605No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG14250.2250783035571849No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA14150.22349880669011693No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA13990.22097161170280816No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG13920.2198659638958606No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT13850.21876031608891303No Hit
GCTTTAGTTTTTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCA13020.20565049209224895No Hit
GCGTTCTATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGT12950.20454484428530134No Hit
CTATATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCACCAACT12890.20359714616506058No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCACCAACTTACAAGGCC12800.2021755989846994No Hit
AACTAAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTATTGATTGG12620.19933250462397709No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC12330.19475196370947997No Hit
GGGTAAAGGTGCAAATATTGGTTACACAGAAAGGCCTTGTAAGTTGGTGA12050.19032937248168968No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTT12020.1898555234215693No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG12010.1896975737348625No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA11820.18669652968743336No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT11340.17911494472550715No Hit
ATATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCACCAACTTA11010.17390260506418284No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA10990.17358670569076926No Hit
ATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTG10880.1718492591369945No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT10850.1713754100768741No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC10740.16963796352309934No Hit
GATTGTATACATGCATATATAGGCATCAAAGCAGGATAGCCACATTGTGT10580.1671107685357906No Hit
GTTTTATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTG10510.16600512072884305No Hit
GTTTTTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA10480.16553127166872264No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC10250.16189842887446634No Hit
GTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT10210.16126663012763914No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT10200.16110868044093235No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT9980.15763378733338282No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT9940.15700198858655565No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATA9870.15589634077960807No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT9770.15431684391254008No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG9630.15210554829864495No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCAC9610.15178964892523134No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA9460.1494204036246294No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA9440.14910450425121582No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG9320.14720910801073428No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC9190.14515576208354591No Hit
ATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGT9080.14341831552977113No Hit
GTATGTCAAAGAATTGTGGGTCTTTTGGGCTTTGCTGCCCCTTTTACACA8960.1415229192892896No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA8810.13915367398868764No Hit
TCCAACGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGATTG8710.13757417712161968No Hit
CATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGG8500.13425723370077697No Hit
GTACTTTACCACAGGAACATATTGTACAAAAACTAAAGCAATGTTTTCGG8480.13394133432736338No Hit
GGGTAAAGGTGCAAATATTGGTTACGCAGAAAGGCCTTGTAAGTTGGTGA8440.13330953558053618No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTC8350.13188798840017502No Hit
CAGGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGT7980.12604384999202353No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG7940.12541205124519636No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7920.12509615187178277No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC7840.12383255437812839No Hit
GGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATT7640.12067356064399246No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTGCAAATATTGGTTACGC7500.11846226503009732No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG7480.11814636565668371No Hit
TCCTGGAAGTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAA7410.11704071784973615No Hit
GGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAA7400.11688276816302935No Hit
GCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTC7340.11593507004278858No Hit
CCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTT7310.11546122098266819No Hit
TTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGG7300.1153032712959614No Hit
CATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAA7200.11372377442889342No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG7190.11356582474218663No Hit
GTGAAAGCCTGCTTAGATTGTATACATGCATATATAGGCATCAAAGCAGG7170.11324992536877303No Hit
CTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTA7090.11198632787511867No Hit
GGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTAC7000.11056478069475749No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6990.1104068310080507No Hit
GTGCAAATATTGGTTACACAGAAAGGCCTTGTAAGTTGGTGAGAAAGTGA6970.1100909316346371No Hit
GCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAA6940.10961708257451672No Hit
ATGTGGCTATCCTGCTTTGATGCCTATATATGCATGTATACAATCTAAGC6810.10756373664732835No Hit
ATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTT6760.10677398821379437No Hit
GTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCA6720.1061421894669672No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG6720.1061421894669672No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG6650.1050365416600196No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6640.10487859197331281No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC6610.10440474291319242No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG6590.10408884353977883No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC6580.10393089385307204No Hit
AATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAAT6580.10393089385307204No Hit
CTTCCAGGAACATCAACTACCAGCACGGGACCATGCAAGACCTGCACGAT6560.10361499447965845No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTGCAAATATTGGTTACAC6530.10314114541953806No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6450.10187754792588367No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC6430.1015616485524701No Hit
GTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA6400.1010877994923497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)