FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394408
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15620.39603659154986715No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13680.3468489482971948No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC12890.32681892862213746No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12120.30729599805277785No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG11880.30121092878440603No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTGTGTAAATAATGCCTAGTTT11330.28726597837772055No Hit
GTATACATGCATATAAAGGCATCAAGGCGGGATAACCACATTGTGTAAAA11200.28396989919068577No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT11140.28244863187359287No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10590.26850368146690734No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10110.2563335429301637No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA9940.25202328553173364No Hit
TCCACAGAGTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA9810.2487272063446989No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG9360.2373177014665017No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGG9050.22945782032818807No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8990.2279365530110951No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8700.22058376097847912No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8460.21449869171010727No Hit
GTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8150.20663881057179367No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT8070.20461045414900308No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7910.20055374130342182No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7250.18381980081539928No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7210.182805622604004No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7120.18052372162836455No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCT7110.1802701770755157No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6930.17570637512423684No Hit
CCTTTTACACAATGTGGTTATCCCGCCTTGATGCCTTTATATGCATGTAT6820.17291738504289975No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTGTGTAAATAATGCCTAGT6760.1713961177258068No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6700.16987485040871383No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6680.16936776130301615No Hit
ATTTACACACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6470.1640433256931908No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6310.15998661284760957No Hit
ATCCTAACTTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5990.15187318715644713No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5780.14654875154662178No Hit
GTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT5590.14173140504249407No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5530.1402101377254011No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5470.13868887040830816No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5450.1381817813026105No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5300.1343786130098781No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5230.1326038011399363No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5190.131589622928541No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5170.13108253382284335No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5150.1305754447171457No Hit
GATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTG5130.13006835561144803No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5090.12905417740005273No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5080.1288006328472039No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5070.1285470882943551No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5030.12753291008295978No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5020.12727936553011096No Hit
GTATTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT4990.12651873187156445No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4970.12601164276586682No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC4940.12525100910732034No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4920.12474392000162268No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG4910.12449037544877387No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4890.12398328634307619No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGTAGATTGACGATATGG4880.12372974179022739No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4830.12246201902598325No Hit
CATATAAAGGCATCAAGGCGGGATAACCACATTGTGTAAAAGGAGCAGCA4760.12068720715604146No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4750.12043366260319263No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4720.11967302894464615No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4700.1191659398389485No Hit
CCTTTTTGGGGTTGAAGTCCCAATCTGGATTGTTTGAGTTGGCTCCGAAC4680.11865885073325085No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTC4680.11865885073325085No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4640.11764467252185554No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4510.11434859333482081No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4500.11409504878197196No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4470.11333441512342549No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4420.11206669235918135No Hit
ATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC4410.11181314780633253No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4410.11181314780633253No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4380.11105251414778605No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4320.1095312468306931No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT4280.10851706861929777No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4260.10800997951360013No Hit
CCTTTATATGCATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAAC4260.10800997951360013No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA4230.10724934585505363No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4200.10648871219650717No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4190.10623516764365834No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA4190.10623516764365834No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC4170.10572807853796069No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4160.10547453398511185No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA4130.10471390032656538No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4060.10293908845662361No Hit
GTCCAAGGGATACTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGAC4050.10268554390377477No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATCCCCGAGATTGAGATCT4040.10243199935092595No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA3980.10091073203383298No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC3980.10091073203383298No Hit
AGTTAGGATAGAACCTAGCAGGCATAATTAATTTCAATCTCCTTTTTTCA3950.1001500983752865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)