FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474623
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26240.552859848764177No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT18790.39589316151977466No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG17820.37545588814701353No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT17050.3592324855727599No Hit
GTATAGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG16480.3472229537970136No Hit
CTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTA15150.3192007129869391No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14700.3097195036902973No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTGAGAGTCCAA14570.3069804876712675No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC14440.3042414716522377No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA12910.27200536004365566No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAACAAC12190.2568354251690289No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCGAGGCGA12060.25409640914999904No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA11370.23955855489514838No Hit
GTTTTCTCAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT10630.22396723294067078No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10520.22164960400149172No Hit
TCCCTATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA10300.2170143461231335No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9940.20942937868582012No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA9430.1986840081496261No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC9260.1951022179708948No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG8940.1883600246932829No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8900.18751725053358137No Hit
GTACTAAAACTCAAGCAATGTTTTCGCAAACTGCCTGTAAATAGACCTAT8880.18709586345373064No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT8790.18519962159440229No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA8630.18182852495559634No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8590.18098575079589485No Hit
ATATGGTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8340.17571841229776056No Hit
CTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAA8160.17192592857910383No Hit
GTTTTAGTACAATATGGTCCTGTGGTAAAGTACCCCAACTTCCAATTACA8010.16876552548022325No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7830.16497304176156655No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAACAATGTCAACGACCGACCT7400.15591321954477555No Hit
GTACAAAAACTCAAGCAATGTTTTCGCAAACTGCCTGTAAATAGACCTAT7320.15422767122537256No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6950.14643201024813376No Hit
CTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT6930.14601062316828303No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6930.14601062316828303No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6890.14516784900858157No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6880.14495715546865617No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6820.14369299422910395No Hit
CCTGCTTAGCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCA6800.14327160714925322No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA6660.14032189759029798No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6650.1401112040503726No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6590.1388470428108204No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6540.1377935751111935No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6430.13547594617201442No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6420.13526525263208905No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6400.13484386555223832No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG6400.13484386555223832No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6360.1340010913925368No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAAGGGGCAGCA6350.13379039785261143No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6270.13210484953320847No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6200.13062999475373085No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6180.13020860767388012No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6150.129576527054104No Hit
GTACAATATGGTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC6050.12746959165485025No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6050.12746959165485025No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA5930.1249412691757458No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5900.1243091885559697No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5870.12367710793619356No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5750.12114878545708911No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG5720.12051670483731297No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCGAGGCGA5650.11904185005783537No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG5620.11840976943805925No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5620.11840976943805925No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5580.11756699527835777No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5530.1165135275787309No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5490.11567075341902941No Hit
CCTCTATGTTTCCCTCGTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5400.11377451155970106No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5370.11314243093992495No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5290.11145688262052197No Hit
ATTTACATACTTTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACA5220.10998202784104436No Hit
GCCTTTCTGTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACG5210.10977133430111899No Hit
CTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTC5080.10703231828208915No Hit
ACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAAC5070.10682162474216378No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5070.10682162474216378No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5050.10640023766231303No Hit
AGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCT5040.10618954412238767No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4940.10408260872313395No Hit
CCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG4940.10408260872313395No Hit
CTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4910.10345052810335781No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT4890.10302914102350708No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4870.10260775394365634No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC4850.1021863668638056No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4850.1021863668638056No Hit
GGATTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4840.1019756733238802No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA4830.10176497978395484No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC4810.10134359270410409No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGCAAACTGCCTGTAAATAGA4800.10113289916417872No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4750.10007943146455185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)