FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530465
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTCTTGAAGTAATGATTAACTGCATGCTCAGG18970.3576107754517263No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT18720.3528979291753461No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTCT16330.30784311877315185No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG16320.3076546049220967No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT15750.29690931541194987No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14800.27900049956170525No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA14710.2773038749022085No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA14640.275984277944822No Hit
GAAACGGACTGAGGCCCACTCCCATAGGAATCTTGCGAAAGCCCAGGATG14520.27372211173215955No Hit
GGATAATAAGGCTTAATGCCTTTATCCAATGGCAAATATTTGGTAAGGTT13790.25996060060512943No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC13310.25091193575447956No Hit
ATCCTAACCTTACCAAATATTTGCCATTGGATAAAGGCATTAAGCCTTAT12080.22772473207468918No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11980.2258395935641371No Hit
CTCTTACGGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11940.22508553815991628No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGACGA11720.22093823343670177No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC11350.21396322094765913No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAA11080.20887334696916854No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAATATGTT10960.20661118075650606No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA10740.20246387603329155No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG10660.2009557652248499No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC10460.19718548820374576No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG10320.19454629428897288No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG10150.19134155882103437No Hit
GTCCATTTCTCCTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGATTCGC10100.19039898956575835No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9780.18436654633199173No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAAT9630.18153883856616365No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAATA9520.17946518620455637No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATA9510.17927667235350117No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA9440.17795707539611472No Hit
CATTTGAACCCCAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9300.17531788148134184No Hit
GTCTTGAAGTAATGATTAACTGCATGCTCAGGATAATAAGGCTTAATGCC9240.1741867983750106No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA9150.17249017371551376No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA8850.16683475818385754No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG8850.16683475818385754No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG8650.16306448116275343No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8560.16136785650325658No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC8530.16080231495009095No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8440.1591056902905941No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGT8430.1589171764395389No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC8130.1532617609078827No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGTCAACTTACAAGGCC8110.15288473320577228No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT7910.14911445618466815No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7730.14572120686567447No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCCAATAAA7640.1440245822061776No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7640.1440245822061776No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7570.14270498524879116No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT7540.14213944369562553No Hit
CAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA7160.1349759173555277No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG7100.13384483424919646No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6820.1285664464196507No Hit
CTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG6730.12686982176015382No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6720.12668130790909862No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT6580.12404211399432573No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACTAAAA6540.1232880585901049No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACGGAGTTGTCAGTC6540.1232880585901049No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6450.12159143393060805No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCATTGGATAAAGGCATTAAGC6430.12121440622849763No Hit
GTCTTTGGGTATACATTTGAACCCCAATAAAACCAAACGTTGGGGCTACT6420.12102589237744242No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6390.12046035082427682No Hit
CTTTACCACAGGAACATATTGTACTAAAAATCAAGCAATGTTTTCGGAAA6390.12046035082427682No Hit
TCCACAGAGTATGTAAATAATGCCTAGTCTTGAAGTAATGATTAACTGCA6370.12008332312216641No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATAC6290.11857521231372475No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT6270.11819818461161434No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6160.11612453225000707No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTG6120.11537047684578625No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6120.11537047684578625No Hit
GTTTTATTGGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT6110.11518196299473103No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC6030.11367385218628938No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5950.11216574137784772No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACT5900.11122317212257171No Hit
GAGTATGTAAATAATGCCTAGTCTTGAAGTAATGATTAACTGCATGCTCA5870.1106576305694061No Hit
CTCCAAAAGACACCAAATACTCAAGCACAGTTTCTCTTCCAAAAGTAAGG5870.1106576305694061No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5810.10952654746307486No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTTCA5680.10707586739935716No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5650.10651032584619155No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5610.10575627044197072No Hit
GTATACATTTGAACCCCAATAAAACCAAACGTTGGGGCTACTCCCTTAAC5550.10462518733563948No Hit
TTCTTCTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5530.10424815963352907No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5470.10311707652719783No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5410.1019859934208666No Hit
GTCCATTTCTCCTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGC5380.10142045186770099No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA5320.10028936876136975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)