FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684405
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45910.6708016452246842No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG44270.6468392253124977No Hit
ATCCTAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAACCTTAT23680.345993965561327No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG19830.2897407236943038No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT19610.28652625273047394No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA16370.23918586217225182No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA16350.23889363753917636No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA16340.23874752522263865No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA16110.23538694194227103No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG15930.23275692024459202No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT15040.2197529240727347No Hit
GTATACATGCATATAAAGGCATCAAGGCGGGATAGCCACATTGTGTAAAA14650.21405454372776353No Hit
GTTCTATCCTAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAAC13850.2023655584047457No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA13820.20192722145513253No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA13720.20046609828975534No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT11590.16934417486722042No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC11370.16612970390339055No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC11320.1653991423207019No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC11200.16364579452224925No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10980.16043132355841935No Hit
ATCCCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG10830.15823963881035352No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG10620.15517128016306134No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT10360.15137235993308054No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA9950.14538175495503394No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA9900.14465119337234533No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGTAAATATTTGGTAAGGTT9760.1426056209408172No Hit
CATGTATACAATCTAAAAAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9630.14070616082582682No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9450.1380761391281478No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9370.13690724059584602No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC9190.13427721889816702No Hit
ATATTTACCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACATGCAG9180.1341311065816293No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA9040.13208553415010119No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG8210.11995821187747022No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8200.11981209956093249No Hit
GGTAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAATTAATTT8170.11937376261131932No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT8110.118497088712093No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT8090.11820486407901755No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7720.1127987083671218No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATA7710.11265259605058409No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7650.11177592215135775No Hit
ATTGACAACAGTGCCAACAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7640.11162980983482002No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG7510.10973034971982965No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC7510.10973034971982965No Hit
GGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA7450.10885367582060329No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7420.10841533887099013No Hit
GGGTAAAGGTGCAGGTATTGTTGGCACAGAAAGGCCTTGTAAGTTGGCGA7300.10666199107253746No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA7160.10461641864100933No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7090.10359363242524529No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT7030.10271695852601896No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATATGTT6980.10198639694333034No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6930.10125583536064174No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6870.10037916146141539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)