FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531790
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22410.42140694635100323No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA21800.409936253032212No Hit
GCATAAACGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGATTC19070.3586001993268019No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16870.3172304857180466No Hit
CCTCCCGCTTACAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA15530.29203256924725923No Hit
ATTTCCGAGAGAGGACAACAGAGTTGTCGGTTCCAATGAGGTTTGCTCCA14580.27416837473438765No Hit
CTCGAAAATACACCTCCTTTCCATGGCTGCTAGGATGTGCTGCCAACTGG13110.24652588427762837No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT12700.23881607401417854No Hit
CCTTTTCGAGGTGTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCC12080.22715733654262021No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT11310.21267793677955585No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA10430.1961300513360537No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10140.19067677090580867No Hit
CCCTTAACTTTATGGGATACGTAATTGGAAGTTGGGGTACTTTACCACAG10030.1886082852253709No Hit
GTATACATGCGTACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA9960.18729197615600143No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9730.18296696064235884No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9290.17469301792060776No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9140.17187235562910172No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9050.1701799582541981No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATTTG8930.16792342842099325No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8690.1634103687545836No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8600.16171797137967994No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT8440.15870926493540682No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG8400.15795708832433855No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC8400.15795708832433855No Hit
CTGTAAGAGGGCCTACGTATTGTTGACATCTATTGATAATGTCTTCCTGC8350.1570168675605032No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACGTATCCCATAAA8240.15494838188006543No Hit
GTTGTCAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8230.15476033772729839No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC8190.15400816111623009No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACA8060.1515635871302582No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8030.15099945467195697No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7970.14987118975535457No Hit
GCATGGGAGGTTGGTCTTCCACACCTCGAAAAGGCATGGGGACGAATCTT7940.14930705729705335No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7750.14573421839447903No Hit
ATCCCGATTGGGACTTCAACCCCAAAAAGGATCAATGGCCAGAGGCAAAT7290.13708418736719383No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC7180.13501570168675606No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7050.13257112770078414No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTACGCATGTATA7020.13200699524248294No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6990.1314428627841817No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATCTCC6830.12843415633990862No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6750.12692980311777205No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGGA6700.1259895823539367No Hit
GTCTTACATAAGCGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6680.12561349404840255No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCCTGTTCCGG6630.12467327328456722No Hit
ACTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCACCACCAATCGGCAGT6520.12260478760412945No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT6460.12147652268752704No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT6450.12128847853475996No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6370.1197841253126234No Hit
CCTCTCGCTTACAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA6210.11677541886835029No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG6170.116023242257282No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6170.116023242257282No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6130.11527106564621374No Hit
AATCTGGATTGTTTGAGTTGGCTCCGAATGCAGGGTCCAACTGGTGATCG6100.11470693318791252No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6010.11301453581300888No Hit
CTCGGAAATACACCTCCTTTCCATGGCTGCTAGGATGTGCTGCCAACTGG6010.11301453581300888No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC5990.11263844750747476No Hit
GTCTTTAAACACACAGTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACA5980.1124504033547077No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5970.11226235920194062No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5950.11188627089640647No Hit
ACCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGGA5930.11151018259087234No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5910.11113409428533819No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA5890.11075800597980406No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGCGGCCG5870.11038191767426993No Hit
CCGAACGCAGGGTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATTGGG5820.10944169691043458No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG5730.10774929953553095No Hit
ATTTTCGAGAGAGGACAACAGAGTTGTCGGTTCCAATGAGGTTTGCTCCA5720.10756125538276388No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5680.1068090787716956No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5680.1068090787716956No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5610.10549276970232611No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5590.10511668139679196No Hit
CCTCCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5590.10511668139679196No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5550.10436450478572368No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5510.10361232817465542No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5500.10342428402188833No Hit
CTATTGTGGTTTCACATCTCCTGTCTTACTTTTGGAAGAGAAACTGTCCT5480.1030481957163542No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5430.10210797495251885No Hit
CTGCCTGTTCCGGATAATAAGGTTTAATGCCTTTGTCTAAGGGCAAATAT5320.10003948927208109No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)