FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510507
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA36200.7090989937454334No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGGCAGAGAGTCCAA23620.46267729923389883No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT20370.3990150967567536No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC18310.35866305457124No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT17850.34965240437447476No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16790.32888873218192893No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTTTCCATGC15090.2955885031938837No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCCATGTCAACGACCGACCT14980.2934337824946573No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACT14200.27815485390014244No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC14030.27482483100133787No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC13220.258958251307034No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTTTAAATTTTAT13030.2552364610083701No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAC13010.25484469360851075No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC12630.247401113011183No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG12290.24074106721357394No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC11990.2348645562156836No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA11760.23035923111730103No Hit
GTCGTTGACATGGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG11060.2166473721222236No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC11010.2156679536225752No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT11010.2156679536225752No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT10570.20704907082566937No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT10320.20215197832742743No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT10080.1974507695291152No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG9960.19510016512995904No Hit
CTCTTGGACTCTCTGCCATGTCAACGACCGACCTTGAGGCATACTTCAAA9900.19392486293038097No Hit
CCTCAAGGTCGGTCGTTGACATGGCAGAGAGTCCAAGAGTCCTCTTATGT9580.1876565845326313No Hit
CCTATAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT9240.18099653873502225No Hit
CCTAAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG9170.1796253528355145No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACACC8960.17551179513699125No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8850.1733570744377648No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT8690.17022293523888998No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8460.16571761014050737No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA8340.16336700574135124No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA8220.1610164013421951No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTG8120.15905756434289833No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTCTTGTGGTTTCACATTTCTT8100.15866579694303898No Hit
TTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTTCTTGTGGCAATGAT7850.15376870444479704No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7810.15298516964507833No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAA7770.15220163484535962No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG7640.1496551467462738No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7530.14750042604704736No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT7520.14730454234711765No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7510.147108658647188No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG7420.14534570534782087No Hit
GGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGA7370.1443662868481725No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG7350.14397451944831316No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC7090.13888154325014151No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCTTAT7070.13848977585028216No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT6950.13613917145112606No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT6920.135551520351337No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6840.13398445075189958No Hit
GACGGGGAGGCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG6800.13320091595218086No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6770.13261326485239183No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT6700.1312420789528841No Hit
GTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACA6670.13065442785309506No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGT6570.12869559085379828No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA6550.12830382345393893No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT6430.1259532190547828No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6320.12379849835555633No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6180.12105612655654086No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6060.11870552215738472No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6010.11772610365773634No Hit
CTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC6000.11753021995780666No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTGG5980.11713845255794729No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5930.1161590340582989No Hit
GTTTGTAGGCCCACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTA5920.11596315035836922No Hit
GTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTT5920.11596315035836922No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGGCAGACGGAGAAGG5880.11517961555865051No Hit
CATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCC5840.1143960807589318No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG5820.11400431335907245No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATT5690.11145782525998664No Hit
CACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTATGCCTGCTAGG5650.11067429046026792No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGGTGTAAATGTATGTT5640.11047840676033825No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA5640.11047840676033825No Hit
GATTTGGTGGGCGTTCACGGTGGTTTCCATGCGACGTGCAGAGGTGAAGC5600.10969487196061953No Hit
ATCCATAACATCCAATGACATAACCCATAAAATTTAAAGAATAACCCCAT5540.10851956976104148No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT5450.10675661646167436No Hit
GGCATAATCAATTGCAATCTTCTTTTTTCATTAACTGTGAGTGGGCCTAC5440.1065607327617447No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG5420.10616896536188535No Hit
ACATAAGAGGACTCTTGGACTCTCTGCCATGTCAACGACCGACCTTGAGG5370.10518954686223696No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC5370.10518954686223696No Hit
CCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC5330.10440601206251825No Hit
TGGAAACCACCGTGAACGCCCACCAAATCTTGCCCAAGGTCTTACATAAG5330.10440601206251825No Hit
CTCTAAGGCATCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA5320.10421012836258857No Hit
CCTCTGCACGTCGCATGGAAACCACCGTGAACGCCCACCAAATCTTGCCC5300.1038183609627292No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5300.1038183609627292No Hit
GGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTTTCATTAAC5250.10283894246308081No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)