FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510507
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN77451.5171192559553544No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA32610.6387767454706791No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT18660.3655189840687787No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGGCAGAGAGTCCAA18600.3643436818692006No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCCATGTCAACGACCGACCT17430.34142528897742835No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT16990.3328064061805225No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC14820.29029964329578245No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAC14130.27678366800063464No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC13470.263855343805276No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC12690.24857641521076104No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG12530.24544227601188623No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACT12250.23995753241385526No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTTTAAATTTTAT11450.22428683641948105No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT11310.22154446462046554No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC10150.1988219554286229No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA10120.19823430432883388No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT9930.19451251403017No Hit
CTCTTGGACTCTCTGCCATGTCAACGACCGACCTTGAGGCATACTTCAAA9830.19255367703087323No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTTTCCATGC9530.1866771660329829No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA9520.18648128233305322No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG9470.18550186383340483No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT9300.1821718409346003No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT9080.1778623995361474No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA9020.17668709733656934No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT8890.17414060923748353No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8750.17139823743846802No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG8610.16865586563945253No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACACC8410.16473819164085898No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTG8250.16160405244198414No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCTTAT8140.1594493317427577No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG8020.15709872734360156No Hit
TTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTTCTTGTGGCAATGAT7880.15435635554458607No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA7810.15298516964507833No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7530.14750042604704736No Hit
GTCGTTGACATGGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG7520.14730454234711765No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7180.1406444965495086No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTCTTGTGGTTTCACATTTCTT7150.1400568454497196No Hit
CTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC7000.13711858995077444No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6890.13496386925154796No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG6730.13182973005267312No Hit
CCTCAAGGTCGGTCGTTGACATGGCAGAGAGTCCAAGAGTCCTCTTATGT6700.1312420789528841No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6690.1310461952529544No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT6610.129479125653517No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6560.1284997071538686No Hit
GTTTACGTCCCCTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGTCG6560.1284997071538686No Hit
GTTTGTAGGCCCACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTA6520.1277161723541499No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTCCGACCGACCACGGGGCGCACCT6470.1267367538545015No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6460.12654087015457183No Hit
GTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACA6450.12634498645464215No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG6420.12575733535485312No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6380.1249738005551344No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT6370.12477791685520472No Hit
GCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTCCGACCGACCACG6370.12477791685520472No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT6350.12438614945534537No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA6320.12379849835555633No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6220.12183966135625955No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT6220.12183966135625955No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6190.12125201025647053No Hit
CCTAAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG6110.11968494065703311No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC6080.11909728955724407No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT6080.11909728955724407No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6000.11753021995780666No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5830.11420019705900213No Hit
CCTATAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT5810.11380842965914278No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATT5780.11322077855935374No Hit
ACATAAGAGGACTCTTGGACTCTCTGCCATGTCAACGACCGACCTTGAGG5700.11165370895991633No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5640.11047840676033825No Hit
TGGAAACCACCGTGAACGCCCACCAAATCTTGCCCAAGGTCTTACATAAG5530.1083236860611118No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTGG5480.10734426756146341No Hit
CTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGAC5400.10577719796202598No Hit
CCTCTGCACGTCGCATGGAAACCACCGTGAACGCCCACCAAATCTTGCCC5330.10440601206251825No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5200.10185952396343242No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA5160.10107598916371371No Hit
GTGTAAATGTATGTTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA5140.10068422176385437No Hit
CTGCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGT5120.100292454363995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)