FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284506
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12580.44216993666214416No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA9120.32055562975824764No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA8770.3082536044934026No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC8600.3022783350790493No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC8430.296303065664696No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8350.29349117417558857No Hit
GTGTAAAAGGTGCAGCAAAACCCAAGAGGCCCACAATTCGTTGACACACT8300.29173374199489643No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8020.28189212178302037No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC7870.2766198252409439No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC7560.26572374572065266No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC7080.248852396786008No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT6540.22987212923453287No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATGCTGGATCTTC6380.22424834625631795No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT6070.21335226673602664No Hit
CACTAATATGGGCCTAAAATTCAGACAACTCTTGTGGTTTCACATTTCTT5980.2101888888107808No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT5820.20456510583256593No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5790.20351064652415063No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTTTCCATGC5770.20280767365187377No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCTCTAAATTTTAT5640.1982383499820742No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5630.1978868635459358No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCAAGTACAAAGACC5600.1968324042375205No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5590.19648091780138205No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5270.1852333518449523No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5060.1778521366860453No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCATCAAACTCTGCAAG4880.17152538083555358No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4800.16871348934644612No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4770.16765903003803082No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCA4750.16695605716575398No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT4670.16414416567664655No Hit
CTATGTATCCCTCCTGCTGCTGTACCAAACCTTCGGACGGAAATTGCACC4650.16344119280436967No Hit
GACGGGGAGGCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG4640.16308970636823125No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG4590.16133227418753912No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4550.15992632844298538No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC4550.15992632844298538No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT4530.15922335557070855No Hit
GTTAATAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTT4510.15852038269843166No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4470.15711443695387795No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTGAACAAACAG4410.15500551833704737No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4390.1543025454647705No Hit
GTTATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4390.1543025454647705No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4350.1528965997202168No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4290.15078768110338622No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4280.15043619466724778No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA4270.15008470823110936No Hit
GTATGGATCGGCAGAGGAGCCATAAAGGTTCCACGCATGCGCTGATGGCC4270.15008470823110936No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4250.1493817353588325No Hit
CCTCTATGTATCCCTCCTGCTGCTGTACCAAACCTTCGGACGGAAATTGC4210.14797578961427876No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATAA4180.14692133030586352No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4160.14621835743358663No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4140.14551538456130977No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4100.14410943881675606No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4060.14270349307220234No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGGCAGACGGAGAAGG4040.14200052019992548No Hit
CTCTAAGGCATCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA3970.1395401151469565No Hit
GTAGTAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT3940.1384856558385412No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA3930.13813416940240275No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGCCTCTTGGGTTTT3920.13778268296626434No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAA3910.13743119653012592No Hit
CCTATTAACAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGCCT3900.13707971009398748No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTG3880.13637673722171062No Hit
ATGTATATTTGCGGGAGAGGACAACAGAGTTATCAGTCCCGAGAATGTTT3860.13567376434943373No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC3820.13426781860488005No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA3820.13426781860488005No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGT3790.13321335929646475No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC3770.1325103864241879No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG3740.13145592711577261No Hit
GGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGGCGATTGGTGGAGGC3720.13075295424349573No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA3700.1300499813712189No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC3660.12864403562666515No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAGAGGCCCACAAT3650.12829254919052674No Hit
GCCATAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC3620.12723808988211147No Hit
CTCCAAATCTCCTCCCCCAACTCCTCCCAGTCTTTGAACAAACAGTCTTT3610.12688660344597302No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3590.12618363057369616No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3550.12477768482914245No Hit
CCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGT3530.12407471195686559No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3460.12161430690389657No Hit
GGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGA3450.12126282046775815No Hit
TTCTACTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC3440.12091133403161973No Hit
CTTCAGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGAGGGAGTTCT3440.12091133403161973No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG3440.12091133403161973No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC3410.11985687472320444No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC3410.11985687472320444No Hit
CAATAGGCCTGTTAATAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT3400.119505388287066No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3400.119505388287066No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG3390.11915390185092756No Hit
CTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTTTTGCCTTCT3390.11915390185092756No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAA3380.11880241541478914No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG3360.11809944254251227No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3340.11739646967023543No Hit
GAGTTATCAGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA3310.11634201036182014No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA3310.11634201036182014No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA3280.11528755105340485No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT3280.11528755105340485No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAGCAGGAGGGA3270.11493606461726644No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG3260.114584578181128No Hit
GTCCCAAGCGACCCCGAGAAGGGTCGTCCGCAGGATTCAGCGCCGAGGGG3240.11388160530885114No Hit
GCCTGAGGATGAGTGTTTCGCAAAGGTGGAGACAGCGGGGTAGGCTGCCT3240.11388160530885114No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTGCGGT3230.11353011887271271No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCAAGTTCAAAGACC3210.11282714600043585No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3210.11282714600043585No Hit
GTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC3170.11142120025588213No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTG3130.1100152545113284No Hit
GGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG3120.10966376807518999No Hit
GTTTACGTCCCCTCGGCGCTGAATCCTGCGGACGACCCTTCTCGGGGTCG3100.10896079520291312No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG3100.10896079520291312No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA3070.10790633589449783No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3040.10685187658608253No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT3040.10685187658608253No Hit
TTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTTCTTGTGGCAATGAC3030.10650039014994411No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG3020.10614890371380568No Hit
GTATATTTGCGGGAGAGGACAACAGAGTTATCAGTCCCGAGAATGTTTGC3010.10579741727766724No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3010.10579741727766724No Hit
TAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAGAGGCCCACAATTCG2980.10474295796925195No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC2910.10228255291628296No Hit
GTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGACTGT2880.10122809360786768No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA2850.10017363429945239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)