FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284506
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14310.5029770901140925No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8640.30368428082360305No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8200.2882188776335121No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA7910.2780257709854977No Hit
GTGTAAAAGGTGCAGCAAAACCCAAGAGGCCCACAATTCGTTGACACACT7750.27240198800728277No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7530.26466928641223736No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC7460.26220888135926834No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC7250.25482766620036135No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT6910.24287712737165473No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6420.2256542920008717No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6370.22389685982017954No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCTCTAAATTTTAT6260.2200305090226568No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT6220.2186245632781031No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6170.21686713109741093No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATGCTGGATCTTC6060.21300078029988823No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT5990.2105403752469192No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5740.20175321434345847No Hit
CACTAATATGGGCCTAAAATTCAGACAACTCTTGTGGTTTCACATTTCTT5710.2006987550350432No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC5630.1978868635459358No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC5480.19261456700385932No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5370.1887482162063366No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCA5320.18699078402564445No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5320.18699078402564445No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCAAGTACAAAGACC5150.18101551461129115No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5120.17996105530287584No Hit
CTATGTATCCCTCCTGCTGCTGTACCAAACCTTCGGACGGAAATTGCACC5110.17960956886673743No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4940.17363429945238415No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4910.17257984014396885No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4810.16906497578258456No Hit
GTATGGATCGGCAGAGGAGCCATAAAGGTTCCACGCATGCGCTGATGGCC4600.16168376062367754No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT4490.1578174098261548No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTTTCCATGC4490.1578174098261548No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG4470.15711443695387795No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4460.15676295051773953No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCATCAAACTCTGCAAG4460.15676295051773953No Hit
GTTATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4440.15605997764546267No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAGAGGCCCACAAT4380.1539510590286321No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT4350.1528965997202168No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4320.15184214041180152No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTG4270.15008470823110936No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4250.1493817353588325No Hit
GTTTACGTCCCCTCGGCGCTGAATCCTGCGGACGACCCTTCTCGGGGTCG4190.14727281674200193No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4160.14621835743358663No Hit
ATGTATATTTGCGGGAGAGGACAACAGAGTTATCAGTCCCGAGAATGTTT4130.14516389812517133No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATAA4090.14375795238061764No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4080.1434064659444792No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC4040.14200052019992548No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4040.14200052019992548No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4010.14094606089151018No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3990.14024308801923333No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTGAACAAACAG3980.1398916015830949No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA3960.13918862871081805No Hit
TTCTACTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC3940.1384856558385412No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC3940.1384856558385412No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTCCGACCGACCACGGGGCGCACCT3920.13778268296626434No Hit
GGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGGCGATTGGTGGAGGC3850.13532227791329532No Hit
GTTAATAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTT3840.1349707914771569No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC3840.1349707914771569No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3840.1349707914771569No Hit
CTCTAAGGCATCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA3830.13461930504101846No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG3830.13461930504101846No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGCCTCTTGGGTTTT3760.13215889998804947No Hit
CAAATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCC3730.13110444067963417No Hit
CCTCTATGTATCCCTCCTGCTGCTGTACCAAACCTTCGGACGGAAATTGC3730.13110444067963417No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC3710.1304014678073573No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA3670.1289955220628036No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG3660.12864403562666515No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3570.1254806577014193No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3550.12477768482914245No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA3540.124426198393004No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG3490.12266876621231186No Hit
GTAGTAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT3470.12196579334003502No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC3460.12161430690389657No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG3450.12126282046775815No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3440.12091133403161973No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTGCGGT3420.12020836115934286No Hit
GCCATAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC3420.12020836115934286No Hit
CTGTTGTCCTCTCCCGCAAATATACATCGTTTCCATGGCTGCTAGGCTGT3410.11985687472320444No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTG3390.11915390185092756No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA3380.11880241541478914No Hit
CCTATTAACAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGCCT3370.11845092897865071No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT3360.11809944254251227No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3350.11774795610637386No Hit
CCGACTACTGCCTCTCACTTATCGTCAATCTTCTCGAGGATTGGGGACCC3320.11669349679795857No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC3300.11599052392568172No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3280.11528755105340485No Hit
GACGGGGAGGCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG3260.114584578181128No Hit
GCCTGAGGATGAGTGTTTCGCAAAGGTGGAGACAGCGGGGTAGGCTGCCT3260.114584578181128No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAA3250.11423309174498955No Hit
GTCCCAAGCGACCCCGAGAAGGGTCGTCCGCAGGATTCAGCGCCGAGGGG3240.11388160530885114No Hit
GCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTCCGACCGACCACG3230.11353011887271271No Hit
CTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTTTTGCCTTCT3220.11317863243657426No Hit
CAATAGGCCTGTTAATAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT3180.11177268669202056No Hit
GTATATTTGCGGGAGAGGACAACAGAGTTATCAGTCCCGAGAATGTTTGC3170.11142120025588213No Hit
GTCTCCACCTTTGCGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCA3120.10966376807518999No Hit
CTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC3110.10931228163905155No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA3110.10931228163905155No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA3110.10931228163905155No Hit
TTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTTCTTGTGGCAATGAC3070.10790633589449783No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGGCAGACGGAGAAGG3070.10790633589449783No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCAAGTTCAAAGACC3060.10755484945835941No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCTTAT3050.10720336302222097No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA3040.10685187658608253No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT3030.10650039014994411No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC3030.10650039014994411No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA3010.10579741727766724No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA3000.10544593084152883No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGAAC2950.10368849866083667No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA2950.10368849866083667No Hit
ATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGCA2940.10333701222469825No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC2940.10333701222469825No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAGCAGGAGGGA2930.10298552578855982No Hit
CCCCAAAAGACCTCCGTGCGGTGGGGTGAAACCCAGCCCGAATGCTCCAG2930.10298552578855982No Hit
TATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCG2920.10263403935242138No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG2920.10263403935242138No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT2890.1015795800440061No Hit
GAGTTATCAGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA2890.1015795800440061No Hit
CTTTATGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTT2890.1015795800440061No Hit
CCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGT2880.10122809360786768No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG2870.10087660717172924No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCACTTATCGTCAATCTTCTCG2850.10017363429945239No Hit
GTCATGGGCCATCAGCGCATGCGTGGAACCTTTATGGCTCCTCTGCCGAT2850.10017363429945239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)