FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522950
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGG41720.7977818147050387No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGGTGTTCTGGAT18840.3602638875609523No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT18460.35299741849125155No Hit
ATATAAGACCTTGGGCAATATTTGGTGGGCGTTCACGGTGGTCTCCATGC13230.25298785734773876No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACACCTAGTTAATCATTACT13010.24878095420212257No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12970.24801606272110144No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12810.24495649679701692No Hit
GTCTTATATAAGAGGACTCTTGGACTTTCTGCAATGTCAACGACCGACCT12450.23807247346782678No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT12370.2365426905057845No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC12250.23424801606272108No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11950.22851132995506263No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG11790.2254517640309781No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT11670.22315708958791475No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTCTTGTGGTTTCACATTTCTT11170.21359594607515056No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT11130.2128310545941295No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10670.20403480256238646No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10650.2036523568218759No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT10210.19523855053064346No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTAT9980.19084042451477196No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC9480.18127928100200785No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9320.1782197150779233No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA8730.16693756573286164No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8680.16598145138158524No Hit
GTATTAAACCTTATTATCCAGAACACCTAGTTAATCATTACTTCCAAACT8650.1654077827708194No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT7820.14953628453963094No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT7590.14513815852375944No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCC7340.14035758676737736No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT7190.13748924371354815No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA7180.13729802084329287No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT7060.13500334640022948No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG6810.1302227746438474No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA6710.12831054594129457No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC6620.12658954010899703No Hit
TTCTAAAACAGTCTTTGATTTTTTGTCTGATGTGTTCTTGTGGCAATGAC6490.12410364279567836No Hit
TCTAGGGACCTAGTAGTCGGTTATGTCAACACTAATATGGGCCTAAAGTT6460.12352997418491252No Hit
ATATTAGTGTTGACATAACCGACTACTAGGTCCCTAGATGCTTGATCTTC6450.12333875131465724No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTACGCGGGACGTCCTTTGTTTA6180.11817573381776461No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAAAGTCCAA6150.11760206520699874No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACCGCACTCAGGCA6060.11588105937470122No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA6000.11473372215316952No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGGTGTTCTGG5980.11435127641265894No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTG5960.1139688306721484No Hit
TTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATG5930.11339516206138253No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT5850.11186537909934027No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTGG5850.11186537909934027No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT5840.111674156229085No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGGTGTAAATGTATATT5830.11148293335882971No Hit
CTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTA5740.10976192752653217No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAA5680.10861459030500048No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5670.1084233674347452No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTAACTGTGAGTGGGCCTAC5670.1084233674347452No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5670.1084233674347452No Hit
GTTTTATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC5600.10708480734295822No Hit
TTATTATCCAGAACACCTAGTTAATCATTACTTCCAAACTAGACACTATT5580.10670236160244764No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT5580.10670236160244764No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5480.10479013289989483No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5420.10364279567836314No Hit
TTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCT5330.10192178984606559No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5280.10096567549478919No Hit
CTCCGAAAGACACCAAATATTCTATAACTGTTTCTCTTCCAAAAGTGAGA5260.10058322975427861No Hit
GTTATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5240.10020078401376804No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5230.10000956114351277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)