FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526867
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA25760.48892794576240306No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT15410.29248368184000895No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA14780.28052620490560237No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT14160.2687585291923768No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14020.26610131209584204No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT12970.246172183871831No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT12310.23364530327388125No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA10870.20631392742380905No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACCACACGTAGCGCC10500.19929128224010995No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10350.19644426392239409No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9900.1879032089692465No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT9670.1835377808820822No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC9620.1825887747761769No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT9280.17613553325602097No Hit
CCTCAGCATTGTTCACCTCATCATACTGCACTCAGGCAAGCAATCCTGTG9050.17177010516885666No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT8960.17006189417822715No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC8710.1653168636487007No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT8650.16417805632161436No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8380.15905342334972583No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8240.15639620625319103No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT8130.1543083928201994No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8070.15316958549311308No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT8050.15278998305075095No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8020.15222057938720776No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGTTT7990.1516511757236646No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG7970.15127157328130247No Hit
GGGTAAAGGTTCAGGTATTGTTTACATAGAAAGGCCTTGTAAGTTGGCGA7970.15127157328130247No Hit
GCGTGGGACCATGCAGAACCTGCACGACGACTGTTCAAGGAACCTCTATG7960.15108177206012144No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT7920.15032256717539721No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT7670.14557753664587078No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7450.1414019097798875No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA7330.13912429512571484No Hit
CCTGAGCATTGTTCACCTCATCATACTGCACTCAGGCAAGCAATCCTGTG7290.1383650902409906No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7230.13722628291390426No Hit
GTTTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG7210.13684668047154214No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7200.1366568792503611No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC7130.1353282707020937No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7020.13324045726910205No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC6980.13248125238437783No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6950.13191184872083467No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6900.13096284261492938No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCTGGAT6820.12944443284548093No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCCACCAATCGCCAGTC6770.12849542673957565No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC6680.12678721574894614No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6660.126407613306584No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6560.12450960109477345No Hit
ATATAAGAGAGAAACCACACGTAGCGCCTCATTTTGTGGGTCACCATATT6490.12318099254650604No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATTTAGTTAATCATTACT6460.12261158888296286No Hit
AATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGG6430.12204218521941972No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6270.11900536568052279No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA6260.11881556445934173No Hit
ATGCTGAGGAGACTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCAA6180.11729715468989327No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6070.11520934125690163No Hit
CCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGT6020.11426033515099636No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT6010.11407053392981531No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5950.11293172660272895No Hit
GTATTAAACCTTATTATCCAGAACATTTAGTTAATCATTACTTCCAAACC5880.11160311805446158No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA5850.11103371439091839No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5810.11027450950619416No Hit
AATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGTCT5800.11008470828501311No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5800.11008470828501311No Hit
ACATAGAGGTTCCTTGAACAGTCGTCGTGCAGGTTCTGCATGGTCCCACG5790.10989490706383204No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5720.10856629851556465No Hit
GATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGAGTAACTCC5670.10761729240965935No Hit
GTCCGGCAGATGAGAAGGTACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5670.10761729240965935No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5650.10723768996729725No Hit
TCCTTGAACAGTCGTCGTGCAGGTTCTGCATGGTCCCACGCTGGTGGTTG5640.1070478887461162No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5630.10685808752493513No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5560.10552947897666774No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5470.10382126798603822No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC5420.10287226188013293No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5310.1007844484471413No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA5300.10059464722596025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)