FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526867
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA27580.5234717680173554No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA14670.27843839147261074No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT14160.2687585291923768No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13900.26382369744166934No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT12940.24560278020828788No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT12890.24465377410238257No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT12510.23744132769750242No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC12110.22984927885026013No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA11360.21561418726168086No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10760.20422611399081742No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACCACACGTAGCGCC10570.20061989078837733No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT10020.1901808236234192No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9790.18581539553625487No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT9730.18467658820916852No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT9480.1799315576796421No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC9320.1768947381407452No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT9220.17499672592893464No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9150.17366811738066723No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG8740.1658862673122439No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCTGGAT8440.16019223067681218No Hit
CCTCAGCATTGTTCACCTCATCATACTGCACTCAGGCAAGCAATCCTGTG8350.15848401968618267No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGTTT8060.15297978427193198No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA8000.15184097694484566No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7960.15108177206012144No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7840.14880415740594874No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7710.146336741530595No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT7550.1432999219916981No Hit
GCGTGGGACCATGCAGAACCTGCACGACGACTGTTCAAGGAACCTCTATG7470.14178151222224963No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7370.13988350001043906No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7300.13855489146217165No Hit
GGGTAAAGGTTCAGGTATTGTTTACATAGAAAGGCCTTGTAAGTTGGCGA7270.13798548779862851No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7250.1376058853562664No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7080.1343792645961884No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7020.13324045726910205No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7000.13286085482673995No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6990.13267105360555892No Hit
GTATTAAACCTTATTATCCAGAACATTTAGTTAATCATTACTTCCAAACC6910.13115264383611044No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6770.12849542673957565No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6750.12811582429721355No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATTTAGTTAATCATTACT6660.126407613306584No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6560.12450960109477345No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA6550.1243197998735924No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA6490.12318099254650604No Hit
GTTTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG6490.12318099254650604No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACT6470.12280139010414394No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6400.12147278155587654No Hit
CCTGAGCATTGTTCACCTCATCATACTGCACTCAGGCAAGCAATCCTGTG6390.12128298033469548No Hit
ATATAAGAGAGAAACCACACGTAGCGCCTCATTTTGTGGGTCACCATATT6270.11900536568052279No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6040.11463993759335848No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6000.11388073270863425No Hit
AATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGG5960.11312152782391002No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC5950.11293172660272895No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC5940.1127419253815479No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5900.11198272049682367No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA5780.10970510584265099No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5760.10932550340028889No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5740.10894590095792676No Hit
CTCTTTACGCGGACTCCCCGTCTGTACCTTCTCATCTGCCGGACCGTGTG5660.1074274911884783No Hit
AATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGTCT5620.10666828630375408No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5590.1060988826402109No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA5580.10590908141902984No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT5560.10552947897666774No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCCACCAATCGCCAGTC5550.10533967775548667No Hit
ACATAGAGGTTCCTTGAACAGTCGTCGTGCAGGTTCTGCATGGTCCCACG5490.10420087042840034No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5420.10287226188013293No Hit
ATGCTGAGGAGACTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCAA5400.10249265943777083No Hit
GTTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGA5360.1017334545530466No Hit
CTTTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA5320.10097424966832237No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5310.1007844484471413No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC5280.10021504478359815No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5270.10002524356241707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)