FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422080
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA24640.5837755875663381No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT12190.2888078089461713No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC12180.28857088703563305No Hit
GGATAATAGGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT11380.2696171341925701No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG11150.26416793025018953No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC10180.24118650492797575No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10070.23858036391205456No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9970.2362111448066717No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8900.21086050037907506No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8540.20233131159969672No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8230.19498673237300987No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTACATTTCAT8090.19166982562547386No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAAC7980.1890636846095527No Hit
CTCTATAACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA7790.18456216830932526No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7790.18456216830932526No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7400.17532221379833207No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC7150.16939916603487493No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7110.16845147839272176No Hit
GTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAACATACATTTACA7040.16679302501895374No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6950.16466072782410918No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT6850.1622915087187263No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6680.15826383623957543No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTC6530.15471000758150114No Hit
GTTCTGGATAATAGGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA6340.1502084912812737No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC6190.1466546626231994No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6140.14547005307050798No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG5950.1409685367702805No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5790.13717778620166793No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT5730.1357562547384382No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5710.13528241091736165No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5690.13480856709628505No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC5570.13196550416982564No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA5520.13078089461713419No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5510.1305439727065959No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5350.12675322213798332No Hit
GTATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5310.12580553449583018No Hit
GTGTAAATGTATGTTAGGAAAAGAAGGTGTTTTCCAATGAGGGTTAAAGA5230.12391015921152389No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTT5190.12296247156937073No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA5170.12248862774829417No Hit
CCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCCTGGGCTTT5050.11964556482183472No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAACATACA4980.11798711144806673No Hit
AGATAAGGGTATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACT4920.116565579984837No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4910.11632865807429871No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC4880.11561789234268385No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4820.11419636087945412No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4750.11253790750568612No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4670.11064253222137982No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4640.10993176648976498No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA4560.10803639120545867No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4540.10756254738438212No Hit
TTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCT4430.10495640636846094No Hit
CTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAACATACATTTACACC4410.10448256254738439No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC4400.1042456406368461No Hit
CTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT4360.10329795299469295No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT4340.10282410917361638No Hit
ATTTGTAGGTCCACTCACAGTCAATGAGAAACGAAGACTGCAATTAATTA4330.10258718726307808No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4280.10140257771038665No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4280.10140257771038665No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4270.10116565579984836No Hit
GTCCAAAGGTTTGGTACAGCAACAGGAGGGATACATAGAGGTTCCTTGAA4260.10092873388931009No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4250.1006918119787718No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)