FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570624
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG29910.5241630215343203No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26150.458270244504262No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAACTGATTATGCCTGCTAGG25130.44039507626738444No Hit
ATCCTAACCTAACTAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT22180.388697285778376No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA20810.36468848138178556No Hit
GTTCTATCCTAACCTAACTAAATATTTGCCCTTAGATAAAGGCATTAAAC19010.3331440668461193No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG18180.3185985868102288No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTAGTTAGGTT16650.2917858344549125No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA14750.2584889524450426No Hit
GGATAGAACCTAGCAGGCATAATCAGTTTTAATCTCCTTTTTTCATTAAC14250.24972661507402422No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA14210.24902562808434275No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT13280.23272768057424853No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT13130.23009897936294302No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT13040.22852175863615973No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA12790.2241405899506505No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAGTTTTAATCTCCTTTTTTCA12650.22168713548676536No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT11800.2067911619560341No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11640.2039872139973082No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT11190.19610111036339165No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT10860.19031796769851952No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA10780.18891599371915657No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC10760.18856550022431584No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC10530.18453482503364738No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10230.17927742261103632No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA10130.17752495513683267No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTAGTT10010.17542199416778823No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC9920.17384477344100494No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG9860.1727932929564827No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC9840.172442799461642No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG9840.172442799461642No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA9760.17104082548227906No Hit
GATTAAAACTGATTATGCCTGCTAGGTTCTATCCTAACCTAACTAAATAT9090.1592992934051144No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAATTATT9010.15789731942575147No Hit
AACTAAATATTTGCCCTTAGATAAAGGCATTAAACCTTATTATCCTGAAC8930.15649534544638852No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA8900.1559696052041274No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAACTGATTATGCCTGCTAGGTT8860.15526861821444593No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC8830.15474287797218483No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT8700.15246467025572005No Hit
TTATTATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACATTATT8700.15246467025572005No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA8550.14983596904441454No Hit
CCATCTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA8490.14878448855989232No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGA8180.14335183938986093No Hit
ATCTAAGGGCAAATATTTAGTTAGGTTAGGATAGAACCTAGCAGGCATAA8120.1423003589053387No Hit
GATTATGCCTGCTAGGTTCTATCCTAACCTAACTAAATATTTGCCCTTAG7750.1358162292507851No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT7630.1337132682817407No Hit
CCGAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTT7580.13283703454463885No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7190.1260024113952445No Hit
CTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATAT7160.1254766711529834No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT7090.12424994392104083No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7040.123373710183939No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7030.12319846343651862No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7000.12267272319425751No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAAC6970.12214698295199641No Hit
ATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGGAT6890.12074500897263347No Hit
GTGTAGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAA6870.12039451547779273No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6870.12039451547779273No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA6770.11864204800358905No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT6770.11864204800358905No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC6720.11776581426648722No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTAGTTAGGTTAGGATAGAAC6690.1172400740242261No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6640.11636384028712426No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6480.11355989232839839No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6460.11320939883355764No Hit
TATTTAGTTAGGTTAGGATAGAACCTAGCAGGCATAATCAGTTTTAATCT6440.11285890533871692No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC6440.11285890533871692No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6410.1123331650964558No Hit
ATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACATTATTTACAT6400.11215791834903543No Hit
TATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAAAA6370.11163217810677434No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAG6300.11040545087483176No Hit
GATAAAGGCATTAAACCTTATTATCCTGAACATACAGTTAATCATTACTT6260.10970446388515029No Hit
GGCATAATCAGTTTTAATCTCCTTTTTTCATTAACTGTAAGAGGGCCTAC6260.10970446388515029No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6170.10812724315836697No Hit
GTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6160.10795199641094662No Hit
GCCTTTATCTAAGGGCAAATATTTAGTTAGGTTAGGATAGAACCTAGCAG6020.10549854194706146No Hit
GAATATGGTGACCCACAAAATGAGGCACTGCGTGTAGTTTCTCTCTTATA6000.10514804845222073No Hit
GAAAAAAGGAGATTAAAACTGATTATGCCTGCTAGGTTCTATCCTAACCT5960.10444706146253925No Hit
ATGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG5960.10444706146253925No Hit
ACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATA5900.10339558097801704No Hit
GCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAAC5820.1019936069986541No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5730.10041638627187079No Hit
CCCTCTTACAGTTAATGAAAAAAGGAGATTAAAACTGATTATGCCTGCTA5720.10024113952445043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)