FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570624
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG36180.6340427321668909No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26010.45581679004037684No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAACTGATTATGCCTGCTAGG25570.4481059331538807No Hit
ATCCTAACCTAACTAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT19450.3408549237326155No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA18630.32648469044414535No Hit
GTTCTATCCTAACCTAACTAAATATTTGCCCTTAGATAAAGGCATTAAAC18400.3224540152534769No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTAGTTAGGTT17300.30317687303723645No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG17200.30142440556303274No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA15950.27951856213548676No Hit
GGATAGAACCTAGCAGGCATAATCAGTTTTAATCTCCTTTTTTCATTAAC13590.23816032974427995No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA13260.2323771870794078No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT13150.23044947285778378No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAGTTTTAATCTCCTTTTTTCA12850.22519207043517273No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA12440.21800695379093765No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT12420.21765646029609692No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT12160.21310004486316736No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT11530.20205949977568416No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG11370.19925555181695828No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTAGTT11180.19592586361597128No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA11110.1946991363840287No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT11060.19382290264692686No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC10600.18576155226558994No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA10240.1794526693584567No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC10060.17629822790489008No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9850.17261804620906238No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG9740.17069033198743833No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA9590.1680616307761328No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9570.16771113728129206No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC9480.16613391655450876No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT9390.16455669582772545No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAATTATT9320.16332996859578286No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA9160.16052602063705698No Hit
TTATTATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACATTATT9070.15894879991027366No Hit
GATTAAAACTGATTATGCCTGCTAGGTTCTATCCTAACCTAACTAAATAT9030.1582478129205922No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT8810.1543923844773441No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA8770.1536913974876626No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC8740.15316565724540154No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8630.15123794302377747No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC8170.14317659264244054No Hit
ATCTAAGGGCAAATATTTAGTTAGGTTAGGATAGAACCTAGCAGGCATAA7860.13774394347240917No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAACTGATTATGCCTGCTAGGTT7800.13669246298788693No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGA7730.13546573575594437No Hit
ATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGGAT7700.13493999551368327No Hit
CTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATAT7450.13055882682817407No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTAGTTAGGTTAGGATAGAAC7420.13003308658591295No Hit
CCATCTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA7210.12635290489008524No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA7140.12512617765814266No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7050.12354895693135934No Hit
AACTAAATATTTGCCCTTAGATAAAGGCATTAAACCTTATTATCCTGAAC7040.123373710183939No Hit
GATTATGCCTGCTAGGTTCTATCCTAACCTAACTAAATATTTGCCCTTAG7030.12319846343651862No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT6890.12074500897263347No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6780.11881729475100941No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6760.11846680125616868No Hit
GTGTAGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAA6640.11636384028712426No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC6600.1156628532974428No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6550.11478661956034096No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6540.11461137281292058No Hit
CCGAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTT6510.1140856325706595No Hit
GCCTTTATCTAAGGGCAAATATTTAGTTAGGTTAGGATAGAACCTAGCAG6400.11215791834903543No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT6350.1112816846119336No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6280.11005495737999103No Hit
ATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACATTATTTACAT6210.10882823014804846No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAAC6180.10830248990578734No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6150.10777674966352625No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAG6110.10707576267384476No Hit
CTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTG6080.10655002243158368No Hit
GGCATAATCAGTTTTAATCTCCTTTTTTCATTAACTGTAAGAGGGCCTAC6060.10619952893674293No Hit
GTTTTAATCTCCTTTTTTCATTAACTGTAAGAGGGCCTACAAATTGTTGA6040.10584903544190219No Hit
GCGTGTAGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTA6030.10567378869448182No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6030.10567378869448182No Hit
GAATATGGTGACCCACAAAATGAGGCACTGCGTGTAGTTTCTCTCTTATA5910.10357082772543742No Hit
CCCTCTTACAGTTAATGAAAAAAGGAGATTAAAACTGATTATGCCTGCTA5850.10251934724091522No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC5830.10216885374607447No Hit
GCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAAC5790.10146786675639301No Hit
ATGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG5790.10146786675639301No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACA5750.10076687976671153No Hit
GTTTGGAAGTAATGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCC5740.10059163301929115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)