FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275061
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT16640.6049567186914903No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG12220.44426509028906314No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC8840.3213832568048542No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT8550.3108401409141972No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA8490.3086588065919923No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT8310.3021148036253776No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGTG8180.29738857926060036No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC7820.284300573327371No Hit
GTACGGGACCATGCAAAACTTGCACGACTCCTGCTCAAGGCAACTCTATG7750.28175568328479866No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA7310.26575923158862946No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATGTTAGGAAAGGAAGGAGTTT7140.25957878434238224No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7070.2570338942998099No Hit
CTACAGTACCTATCTTTAATCCTGATTGGCAAACTCCTTCCTTTCCTAAC7020.2552161156979725No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT6980.2537618928165025No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC6930.2519441142146651No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6910.25121700277393016No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG6900.25085344705356266No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA6370.2315849938740861No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG6180.22467743518710398No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATCAATTTCAATCT6140.22322321230563402No Hit
CTTTAATCCTGATTGGCAAACTCCTTCCTTTCCTAACATTCATTTACAAG6090.22140543370379662No Hit
AATCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT5920.21522498645754942No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA5900.21449787501681447No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT5900.21449787501681447No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA5770.2097716506520372No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT5480.19922853476138022No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA5460.19850142332064521No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA5110.18577697310778338No Hit
GGATAGAATCTAGCAGGCATAATCAATTTCAATCTTCTCTTTTCATTTAC4980.18105074874300608No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAG4770.173416078615289No Hit
ATCTAGGGAACCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAAC4640.1686898542505117No Hit
GTATAAAGGGACTCACGATGCTGTACAGATTTGGCCCCCAATACCACATC4420.1606916284024271No Hit
ATGTGGTATTGGGGGCCAAATCTGTACAGCATCGTGAGTCCCTTTATACC4390.15960096124132464No Hit
TCCTCAGGCCGTGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTACAGG4300.1563289597580173No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC4280.15560184831728235No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC4230.15378406971544495No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4190.15232984683397502No Hit
ATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAG4020.14614939958772782No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA4010.14578584386736032No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGATTGGCAAACTCCTTCCTTTC3860.1403325080618481No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3790.13778761801927572No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG3740.13596983941743832No Hit
GTACCTATCTTTAATCCTGATTGGCAAACTCCTTCCTTTCCTAACATTCA3660.13306139365449846No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT3660.13306139365449846No Hit
GGGTAGGATAGAATCTAGCAGGCATAATCAATTTCAATCTTCTCTTTTCA3640.1323342822137635No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAGTACCTATC3590.13051650361192607No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT3580.1301529478915586No Hit
GGGTAAAGGTTCATGTACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGA3450.12542672352678133No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3440.12506316780641386No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG3410.1239725006453114No Hit
GTGCTGGAGAAGGAAAAGGGATGAATAGGCAGAATGCACAGGATTTTTAG3370.12251827776384148No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT3370.12251827776384148No Hit
ATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGA3360.12215472204347401No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT3330.12106405488237154No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC3320.12070049916200407No Hit
CCTCTACAGTACCTATCTTTAATCCTGATTGGCAAACTCCTTCCTTTCCT3320.12070049916200407No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG3280.11924627628053412No Hit
GATCTGGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTG3200.11633783051759428No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC3170.11524716335649184No Hit
GTTCATGTACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA3170.11524716335649184No Hit
GAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCT3170.11524716335649184No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC3160.11488360763612436No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC3130.1137929404750219No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATGGACATTGA3120.11342938475465442No Hit
GCTTTATTCCTCTACAGTACCTATCTTTAATCCTGATTGGCAAACTCCTT3100.11270227331391947No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT3090.11233871759355199No Hit
GAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAA3070.11161160615281701No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA3040.11052093899171457No Hit
GTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAAT2960.10761249322877471No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT2860.10397693602509989No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT2800.10179560170289498No Hit
ATATGGATGATGTGGTATTGGGGGCCAAATCTGTACAGCATCGTGAGTCC2800.10179560170289498No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG2770.10070493454179255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)