FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677558
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN73171.0799075503499331No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTAT49070.7242184432919396No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT40610.5993582837188846No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC36100.5327957163814758No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT33060.48792870868619365No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG31000.4575254074189959No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT29580.43656779198238377No Hit
GGGTAAAGGTTCAGGTATTGTTTACGCAGAAAGGCCTTGTAAGTTGGCGA23310.3440295886108643No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTAT22740.33561702466799886No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTG22700.33502666930358727No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAAT21840.32233402896873775No Hit
TTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTTCTTGTGGCAATGAC21160.3122979877737404No Hit
GAATAACCCCATCTCTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA18590.27436765561029464No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC17540.2588708272944899No Hit
GTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTT17320.2556238727902261No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATT17190.25370521785588834No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA16960.2503106745105216No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT16800.24794925305287518No Hit
ACCCATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCAT16620.24529265391302293No Hit
GGGTTATTCTCTAAATTTTATGGGTTATGTCATTGGATGTTATGGGTCAT16450.24278364361427363No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA15360.2266964599340573No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC15100.22285915006538184No Hit
GTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGACTGT14510.21415140844031066No Hit
GTGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTTCTCGT14320.2113472204593555No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG13920.20544366681523943No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG13900.20514848913303366No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG13870.20470572260972492No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC13840.20426295608641623No Hit
ATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCATCTCT13800.2036726007220046No Hit
GCCATAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC13800.2036726007220046No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA13790.20352501188090172No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG13780.2033774230397988No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC13770.20322983419869592No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG13700.20219671231097558No Hit
TAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCG13690.20204912346987267No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA13690.20204912346987267No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG13480.19894975780671173No Hit
GGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAA13330.19673592519016825No Hit
GACCCATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCA13240.1954076256202421No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGTGC13180.19452209257362468No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG13000.19186549343377246No Hit
GAGATGGGGTTATTCTCTAAATTTTATGGGTTATGTCATTGGATGTTATG12960.19127513806936083No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC12880.19009442734053764No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT12750.18817577240619993No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACGC12630.1864047063129651No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC12470.18404328485531865No Hit
GACATAACCCATAAAATTTAGAGAATAACCCCATCTCTTTGTTTTGTTAG12420.18330534064980414No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGCCTTTTGGGTTTT11960.17651625395907067No Hit
GTCTTTGATTTTTTGTATGATGTGTTCTTGTGGCAATGACCCATAACATC11960.17651625395907067No Hit
GGTATTGTTTACGCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC11850.17489277670693873No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTGTGTCAA11600.1712030556793662No Hit
CACTTATCGTCAATCTTCTCGAGGATTGGGGACCCTGTGCTGAACATGGA11390.16810369001620526No Hit
CACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGTGCTGAACATGGA11240.16588985739966172No Hit
GGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAA11010.162495314054295No Hit
GGTTATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACA10950.1616097810076776No Hit
CAATTCGTTGACACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT10780.15910077070892825No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC10520.15526346084025278No Hit
TTGACACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC10470.1545255166347383No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAA10400.15349239474701798No Hit
CATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCATCTCTTTGT10260.15142615097157733No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC10190.15039302908385704No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT10190.15039302908385704No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC10160.14995026256054833No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA10000.14758884110290188No Hit
TTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTATGG9690.14301358702871195No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA9530.1406521655710655No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC9350.13799556643121327No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC9320.13755279990790456No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGCGTAAACAATACC9320.13755279990790456No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9210.13592932265577265No Hit
CATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCA9050.1335679011981262No Hit
CCCATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCATC8960.1322396016282001No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT8940.1319444239459943No Hit
GTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAAT8820.13017335785275946No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA8790.12973059132945075No Hit
GCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACAT8750.12914023596503915No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA8750.12914023596503915No Hit
GATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGT8660.12781193639511304No Hit
GATGTGTTCTTGTGGCAATGACCCATAACATCCAATGACATAACCCATAA8620.12722158103070144No Hit
CCCCATCTCTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAG8610.12707399218959853No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGT8610.12707399218959853No Hit
ATCAAAGACTGTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAA8600.12692640334849564No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCACTTATCGTCAATCTTCTCG8510.1255981037785695No Hit
GTGTTCTTGTGGCAATGACCCATAACATCCAATGACATAACCCATAAAAT8510.1255981037785695No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA8460.12486015957305499No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACAAAAA8420.12426980420864339No Hit
ACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTT8400.12397462652643759No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC8270.12205597159209987No Hit
GAACACATCATACAAAAAATCAAAGACTGTTTTAGAAAACTTCCTGTTAA8270.12205597159209987No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8210.12117043854548246No Hit
GGATGTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGA8170.12058008318107083No Hit
GGTTCAGGTATTGTTTACGCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG8120.11984213897555634No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC8110.11969455013445343No Hit
GTGAAAGCCTGCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGG8110.11969455013445343No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT8070.11910419477004182No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG8010.11821866172342442No Hit
GGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTAC8000.1180710728823215No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7880.1163000067890867No Hit
CCCATAAAATTTAGAGAATAACCCCATCTCTTTGTTTTGTTAGGGTTTAA7730.11408617417254317No Hit
GCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC7730.11408617417254317No Hit
CTCTAAATTTTATGGGTTATGTCATTGGATGTTATGGGTCATTGCCACAA7630.11261028576151413No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT7600.11216751923820545No Hit
CAAGAACACATCATACAAAAAATCAAAGACTGTTTTAGAAAACTTCCTGT7570.11172475271489674No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7560.11157716387379384No Hit
AAACAAAGAGATGGGGTTATTCTCTAAATTTTATGGGTTATGTCATTGGA7510.11083921966827932No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA7510.11083921966827932No Hit
ATGTGTTCTTGTGGCAATGACCCATAACATCCAATGACATAACCCATAAA7440.10980609778055901No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC7430.10965850893945611No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC7410.1093633312572503No Hit
GCCCAGGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT7320.1080350316873242No Hit
GCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTT7300.10773985400511837No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC7230.10670673211739808No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC7180.10596878791188356No Hit
GTTCTTGTGGCAATGACCCATAACATCCAATGACATAACCCATAAAATTT7160.10567361022967775No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG7100.10478807718306035No Hit
TTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGACTGTT6990.10316459993092843No Hit
CCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTT6970.10286942224872263No Hit
GTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAG6930.102279066884311No Hit
GTGTGTCAACGAATTGTGGGCCTTTTGGGTTTTGCTGCACCTTTTACACA6890.1016887115198994No Hit
GGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGTATGAT6880.1015411226787965No Hit
GGGTTATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCATAC6850.10109835615548779No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT6830.10080317847328199No Hit
CCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGTATGATGT6790.10021282310887039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)