FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677558
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN72781.07415158554692No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTAT43970.6489481343294596No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT40110.5919788416637395No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC35630.5258590408496394No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG34290.5060821361418505No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT32400.4781878451734022No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT31760.4687421593428164No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTAT24750.3652823817296822No Hit
GGGTAAAGGTTCAGGTATTGTTTACGCAGAAAGGCCTTGTAAGTTGGCGA24320.3589360615622574No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAAT23790.3511138529838036No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTG23700.34978555341387746No Hit
TTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTTCTTGTGGCAATGAC20660.3049185457185953No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC19270.28440369680529193No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA18380.2712682899471337No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATT18020.2659550916674292No Hit
GAATAACCCCATCTCTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA17330.25577146163132897No Hit
GTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTT17070.25193415176265355No Hit
GGGTTATTCTCTAAATTTTATGGGTTATGTCATTGGATGTTATGGGTCAT15370.2268440487751602No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT15240.2249253938408225No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC15080.22256397238317605No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA15030.22182602817766156No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC14690.21680800758016286No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG14610.21562729685133966No Hit
GTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGACTGT14530.21444658612251644No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT14250.21031409857163522No Hit
GTGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTTCTCGT14240.2101665097305323No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC14100.20810026595509168No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA14100.20810026595509168No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG14030.20706714406737137No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG13800.2036726007220046No Hit
ACCCATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCAT13510.19939252433002047No Hit
GGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAA13500.19924493548891756No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG13230.1952600367791392No Hit
GTCTTTGATTTTTTGTATGATGTGTTCTTGTGGCAATGACCCATAACATC13110.19348897068590437No Hit
GCCATAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC12920.19068478270494923No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC12810.18906130545281732No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG12810.18906130545281732No Hit
GACCCATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCA12610.18610952863075927No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC12290.18138668571546643No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA12240.18064874150995192No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG12130.17902526425781998No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTGTGTCAA11910.17577830975355616No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGTGC11890.17548313207135036No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACGC11810.17430242134252713No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC11710.17282653293149813No Hit
TAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCG11570.17076028915605748No Hit
ATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCATCTCT11530.17016993379164588No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGCCTTTTGGGTTTT11440.16884163422171977No Hit
CAATTCGTTGACACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT11310.16692297928738203No Hit
GGTTATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACA11290.16662780160517623No Hit
GAGATGGGGTTATTCTCTAAATTTTATGGGTTATGTCATTGGATGTTATG11270.16633262392297044No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG11250.16603744624076464No Hit
GGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAA11230.16574226855855884No Hit
GGTATTGTTTACGCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC10820.15969112607333985No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT10620.1567393492512818No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA10570.1560014050457673No Hit
GTGTTCTTGTGGCAATGACCCATAACATCCAATGACATAACCCATAAAAT10500.15496828315804698No Hit
CACTTATCGTCAATCTTCTCGAGGATTGGGGACCCTGTGCTGAACATGGA10470.1545255166347383No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA10450.1542303389525325No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC10360.15290203938260635No Hit
GACATAACCCATAAAATTTAGAGAATAACCCCATCTCTTTGTTTTGTTAG10320.15231168401819475No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC10230.15098338444826864No Hit
CACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGTGCTGAACATGGA10040.1481791964673135No Hit
GCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACAT9850.14537500848635837No Hit
TTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTATGG9720.14345635355202063No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC9700.14316117586981483No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT9630.14212805398209452No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGCGTAAACAATACC9610.14183287629988872No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAA9570.1412425209354771No Hit
GGTTCAGGTATTGTTTACGCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG9460.13961904368334518No Hit
GATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGT9260.13666726686128713No Hit
GTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAAT9200.13578173381466974No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8950.1320920127870972No Hit
CATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCA8890.13120647974047978No Hit
CATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCATCTCTTTGT8870.13091130205827398No Hit
TTGACACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC8840.1304685355349653No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA8640.12751675871290724No Hit
ATCAAAGACTGTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAA8530.1258932814607753No Hit
GTTCTTGTGGCAATGACCCATAACATCCAATGACATAACCCATAAAATTT8420.12426980420864339No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA8330.12294150463871727No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8300.12249873811540858No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCACTTATCGTCAATCTTCTCG8280.12220356043320275No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA8280.12220356043320275No Hit
CCCCATCTCTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAG8170.12058008318107083No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC8150.12028490549886503No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC8120.11984213897555634No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG8100.11954696129335055No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC8080.11925178361114473No Hit
GATGTGTTCTTGTGGCAATGACCCATAACATCCAATGACATAACCCATAA8080.11925178361114473No Hit
GGATGTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGA8060.11895660592893893No Hit
GTGAAAGCCTGCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGG8020.1183662505645273No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC7970.11762830635901281No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT7960.11748071751790991No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7770.11467652953695477No Hit
GGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTAC7670.11320064112592575No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG7650.11290546344371993No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACAAAAA7650.11290546344371993No Hit
CTCTAAATTTTATGGGTTATGTCATTGGATGTTATGGGTCATTGCCACAA7600.11216751923820545No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA7600.11216751923820545No Hit
CCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTT7510.11083921966827932No Hit
CCCATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCATC7480.11039645314497061No Hit
GGTTATCGTTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT7420.1095109200983532No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGT7370.10877297589283869No Hit
GTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAG7360.1086253870517358No Hit
GCCCAGGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT7350.10847779821063289No Hit
GCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC7280.10744467632291257No Hit
ACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTT7250.10700190979960388No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG7170.10582119907078065No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT7060.10419772181864875No Hit
ATGTGTTCTTGTGGCAATGACCCATAACATCCAATGACATAACCCATAAA7060.10419772181864875No Hit
GGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGTATGAT7060.10419772181864875No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC7000.10331218877203131No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG6940.10242665572541391No Hit
TTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGACTGTT6920.10213147804320812No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC6830.10080317847328199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)