FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296230
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15340.5178408668939675No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA14130.4769942274583938No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATATTTAACCT11830.3993518549775512No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG11190.3777470208959255No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9540.322047058029234No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG9170.3095567633257941No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC8800.29706646862235425No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC8620.290990109036897No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT8490.2866016271140667No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8310.2805252675286095No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT8010.2703980015528475No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA7930.2676973972926442No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7020.2369780238328326No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT6940.23427741957262938No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA6890.23258954191000236No Hit
TATTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGTCCTTAAACACACAG6410.21638591634878301No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT6130.20693380143807175No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6070.20490834824291934No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC5890.1988319886574621No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA5680.19174290247442866No Hit
ATGTAAGAGTCCTCTTATGTAAGACCTTGGGCAGGATCTGATGGGCGTTC5640.19039260034432703No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA5580.18836714714917463No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA5380.18161563649866658No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCCTCCGTCAG5300.17891503223846336No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5130.1731762481855315No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT5010.16912534179522667No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT4970.16777503966512508No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC4880.16473685987239645No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4880.16473685987239645No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC4860.16406170880734566No Hit
AGTCTGTACAGCATCGTGAATCCCTTTATACCGCTGTTACCAATTTTCTT4750.16034837794956622No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT4750.16034837794956622No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC4680.1579853492218884No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC4640.15663504709178677No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA4550.15359686729905817No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC4550.15359686729905817No Hit
GGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCGTCACAGGGTCCCC4350.1468453566485501No Hit
GTATAAAGGGATTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC4300.1451574789859231No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4240.1431320257907707No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT4230.14279445025824528No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA4210.14211929919319446No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG4180.14110657259561826No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA4160.14043142153056745No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG4090.13806839280288966No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4060.13705566620531343No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4050.13671809067278803No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG4010.1353677885426864No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4000.13503021301016102No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA3980.1343550619451102No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG3950.133342335347534No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA3950.133342335347534No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3930.1326671842824832No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG3870.1306417310873308No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTT3840.1296290044897546No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG3830.12929142895722917No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG3810.1286162778921784No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT3700.12490294703439894No Hit
GCTCATCGGAACTGACAATTCTGTCGTCCTCTCGCGGAAATATACATCGT3700.12490294703439894No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC3690.12456537150187354No Hit
GTACTTGAATATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3690.12456537150187354No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT3660.12355264490429733No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC3660.12355264490429733No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA3650.12321506937177193No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC3650.12321506937177193No Hit
TCACCATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGATGA3650.12321506937177193No Hit
GTATTAACATAATTGACTACCAGATCCCTGGATGCTGGATCTTCCAAATT3610.12186476724167032No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA3580.1208520406440941No Hit
GTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTTGC3570.12051446511156871No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG3550.1198393140465179No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA3550.1198393140465179No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT3540.1195017385139925No Hit
GGTTTAAAGATCAGGCAACTGTTGTGGTTTCATATATCTTGCCTTACTTT3510.1184890119164163No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3460.11680113425378928No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT3440.11612598318873847No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT3430.11578840765621308No Hit
ATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGATGACTCTA3410.11511325659116227No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA3370.11376295446106066No Hit
GTACTGTAGAGGAATAAAGCCCTGTAAAGTTTCCCACCTTATGAGTCCAA3370.11376295446106066No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA3350.11308780339600985No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC3350.11308780339600985No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG3330.11241265233095905No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT3330.11241265233095905No Hit
ATATTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGTCCTTAAACACACA3300.11139992573338284No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT3300.11139992573338284No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG3290.11106235020085745No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC3290.11106235020085745No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT3270.11038719913580664No Hit
GTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG3270.11038719913580664No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC3240.10937447253823043No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT3210.10836174594065422No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT3180.10734901934307801No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAAGGGATTC3170.10701144381055261No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC3150.10633629274550181No Hit
CTTTAAACCCATGTTAGTATTAACATAATTGACTACCAGATCCCTGGATG3130.105661141680451No Hit
GTCCAAGAGTCCTCTTATGTAAGAGTCCTCTTATGTAAGACCTTGGGCAG3120.1053235661479256No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT3090.1043108395503494No Hit
GTCTAGGAGATCTCTGACGGAGGGAAAGAAGTCAGAAGGCAAAAACGAGA3090.1043108395503494No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT3050.10296053742024779No Hit
GGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCTGTAAAGTTTCCCAC3030.10228538635519697No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG3030.10228538635519697No Hit
GTCCTTAAACACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACA2980.10059750869256996No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA2970.10025993316004456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)