FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374311
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25360.6775114810945978No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA17470.4667241945868542No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14280.3815009444018477No Hit
ACTACACACTGTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGT10860.2901330711627497No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT9920.2650202638982023No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC9730.2599442709404746No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTAT9510.25406680541047416No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9410.2513952301695649No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT9380.2505937575972921No Hit
GTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG8960.23937314158547304No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAATTCGTTGACATACT8570.22895399814592676No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACTACCGTG8540.228152525573654No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8170.21826769718228958No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC8110.21666475203774402No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8010.21399317679683472No Hit
TTCTAAAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7460.1992995129718336No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC7200.1923534173454694No Hit
ACTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6980.18647595181546897No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6570.17552249332774084No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT6530.17445386323137713No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTGTCAACACTTCCGGA6360.1699121853218313No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT6260.167240610080922No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6100.16296608969546714No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT5860.15655430911728482No Hit
CCTTTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGCAT5810.1552185214968302No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAATCTTTGATTTTTTGT5660.15121115863546622No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGCATTCAATCAAAGCAG5290.14132633024410182No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5240.13999054262364719No Hit
TATCCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAATTCG5100.13625033728637415No Hit
GGGTAAAGGTGCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5040.13464739214182858No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCAGCAAACTCTGCAAG5000.13357876204546487No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4970.1327772894731921No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4960.13251013194910116No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT4770.12743413899137349No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT4740.1266326664191007No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT4720.12609835137091882No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4660.12449540622637326No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAGATATTCTAAAA4650.12422824870228233No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA4570.1220909885095549No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT4470.1194194132686456No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT4440.1186179406963728No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC4380.11701499555182722No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCC4380.11701499555182722No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAAAGAGA4380.11701499555182722No Hit
GGATATCCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAAT4340.1159463654554635No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4340.1159463654554635No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC4340.1159463654554635No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4300.11487773535909977No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4290.11461057783500884No Hit
CTTATAAAGAATTTGGAGCAACTGTGGAGTTACTCTCTTTTTTGCCTTCT4260.11380910526273608No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG4230.11300763269046328No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA4220.11274047516637235No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGCATTCAATCAAAGC4200.1122061601181905No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCA4180.11167184507000863No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4170.1114046875459177No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGACGCCTTAGAGTCTCCTGA4140.1106032149736449No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC4100.1095345848772812No Hit
GTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGATTGT4080.10900026982909933No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTAACTGTGAGTGGGCCTAC4060.10846595478091747No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA4050.10819879725682655No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGGTGTAAATGTATATT4030.10766448220864468No Hit
GTATGGATCGGCAGAGGAGCCATAAAGGTTCCACGCATGCGCTGATGGCC4020.10739732468455375No Hit
GGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGGCGATTGGTGGAGGC4010.10713016716046282No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA3990.10659585211228097No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3990.10659585211228097No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA3980.10632869458819003No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3970.1060615370640991No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG3950.10552722201591724No Hit
TCTAGAGACCTAGTAGTCAGTTATGTCAACACTACTGCGGGCCTAAAGTT3940.10526006449182632No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGTTTT3920.10472574944364445No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT3920.10472574944364445No Hit
CTCCGAAAGACACCAGATATTCTAAAACTGTTTCTCTTCCAAAAGTGAGA3880.10365711934728075No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAGTCTCCTCC3860.10312280429909887No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA3840.10258848925091701No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3800.10151985915455329No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG3790.10125270163046238No Hit
ATACTACACACTGTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCA3760.10045122905818957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)