FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374311
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25290.6756413784259613No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA16820.44935895552094385No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12660.33822142549911705No Hit
ACTACACACTGTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGT10350.2765080374341123No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT9420.2516623876936558No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACTACCGTG9380.2505937575972921No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT9180.2452506071154735No Hit
GTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG9110.24338050444683698No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTAT8860.23670156634456374No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8790.23483146367592722No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAATTCGTTGACATACT8430.22521379280865378No Hit
TTCTAAAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7930.21185591660410727No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7680.20517697850183403No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTGTCAACACTTCCGGA7630.20384119088137936No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC7540.20143677316456104No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC7490.20010098554410638No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT7200.1923534173454694No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT6750.18033132876137756No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC6500.17365239065910434No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA6460.1725837605627406No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6190.1653705074122855No Hit
CCTTTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGCAT6070.16216461712319435No Hit
ACTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5820.1554856790209211No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC5820.1554856790209211No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAATCTTTGATTTTTTGT5800.15495136397273926No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5780.15441704892455738No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT5570.14880674091864785No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5500.14693663825001135No Hit
GGGTAAAGGTGCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5330.14239496034046556No Hit
GGATATCCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAAT5210.13918907005137438No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGCATTCAATCAAAGCAG5210.13918907005137438No Hit
TATCCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAATTCG5190.13865475500319252No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5130.13705180985864696No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT5100.13625033728637415No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT5010.1338459195695558No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA4990.13331160452137392No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCAGCAAACTCTGCAAG4980.13304444699728302No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC4870.1301057142322828No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAGATATTCTAAAA4770.12743413899137349No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT4690.12529687879864604No Hit
CTTATAAAGAATTTGGAGCAACTGTGGAGTTACTCTCTTTTTTGCCTTCT4660.12449540622637326No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA4470.1194194132686456No Hit
TCTAGAGACCTAGTAGTCAGTTATGTCAACACTACTGCGGGCCTAAAGTT4460.11915225574455465No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA4360.11648068050364538No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT4320.11541205040728165No Hit
GTATGGATCGGCAGAGGAGCCATAAAGGTTCCACGCATGCGCTGATGGCC4300.11487773535909977No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4290.11461057783500884No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA4270.114076262786827No Hit
GTTTTAGAATATCTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4260.11380910526273608No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGTTTT4250.11354194773864515No Hit
GTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGATTGT4170.1114046875459177No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4150.11087037249773585No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC4140.1106032149736449No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT4130.11033605744955398No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCC4120.11006889992546305No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCA4110.10980174240137212No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAAAGAGA4110.10980174240137212No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC4040.10793163973273562No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4020.10739732468455375No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4020.10739732468455375No Hit
GAATAACCCCATCTCTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA4010.10713016716046282No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3970.1060615370640991No Hit
GGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGGCGATTGGTGGAGGC3930.10499290696773539No Hit
CTCTAAGGCGTCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA3930.10499290696773539No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT3920.10472574944364445No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3910.10445859191955352No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3910.10445859191955352No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGCATTCAATCAAAGC3880.10365711934728075No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT3860.10312280429909887No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGACGCCTTAGAGTCTCCTGA3820.10205417420273515No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTAACTGTGAGTGGGCCTAC3810.10178701667864422No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC3780.10098554410637145No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG3770.10071838658228052No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3770.10071838658228052No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAGTCTCCTCC3750.10018407153409864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)