FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260771
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11180.42872865464334603No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC9010.34551387999432454No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA8140.3121512744898781No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7950.30486518823028635No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7800.29911301486745073No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAAAGTCCAA7740.29681214552231655No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7660.2937443197288042No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6880.2638330182420591No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6830.26191562712111394No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6440.2469599763777414No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT6110.23430519497950308No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAGTACTCTATGA5830.22356780470221No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5780.22165041358126478No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCTGTAATGTCAACGACCGACCT5160.19787476368154436No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5010.19212259031870876No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTTTTTCCTTCCGTACG4990.19135563387033067No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4700.18023476536884853No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT4680.17946780892047046No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4650.17831737424790334No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4600.17639998312695815No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC4580.17563302667858008No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4510.1729486791092568No Hit
ACTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG4500.17256520088506774No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4450.17064780976412253No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT4340.1664295492980431No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG4320.16566259284966503No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC4080.15645911546912808No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4050.15530868079656096No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA4030.1545417243481829No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3950.15147389855467058No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3950.15147389855467058No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT3940.15109042033048153No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3910.14993998565791442No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG3860.14802259453696923No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAGTACTCTATGA3720.14265389939832265No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG3700.14188694294994458No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3690.14150346472575553No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG3660.14035303005318844No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC3570.1369017260354871No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3530.13536781313873092No Hit
CATTAGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA3520.13498433491454187No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA3500.1342173784661638No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC3490.13383390024197475No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA3470.13306694379359668No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3470.13306694379359668No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3420.1311495526726515No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGACTGTACAGCA3400.1303825962242734No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC3380.12961563977589532No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA3350.1284652051033282No Hit
GAAGATGAGGCATAGCAGCAGGATGAAGAGGAAGATGATAAAACGCCGCA3350.1284652051033282No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG3330.12769824865495014No Hit
GGATACTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA3330.12769824865495014No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG3320.1273147704307611No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC3220.1234799881888707No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3210.12309650996468165No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT3210.12309650996468165No Hit
GGTAAATATTTGGTAACATTAGGATAAAACCTAGCAGGCATAATCAATTG3180.12194607529211454No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3180.12194607529211454No Hit
CATGTATTCAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3170.12156259706792549No Hit
CCTTTATCCAATGGTAAATATTTGGTAACATTAGGATAAAACCTAGCAGG3160.12117911884373644No Hit
CTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAA3140.12041216239535837No Hit
GGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCATTGAC3100.11887824949860222No Hit
GTATGCATGTATTCAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACA3090.11849477127441317No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG3090.11849477127441317No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAAAGTCCAAGAGTCCTCTTATGT3070.1177278148260351No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC3050.11696085837765702No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT3040.116577380153468No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT3040.116577380153468No Hit
GTCATAGAGTACTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3030.11619390192927895No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3030.11619390192927895No Hit
ATCTAGAAGATCTCGTACGGAAGGAAAAAAGTCAGAAGGCAAAAATGAGA3020.11581042370508991No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3000.11504346725671181No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA2990.1146599890325228No Hit
AGACTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT2960.11350955435995566No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA2930.11235911968738854No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT2930.11235911968738854No Hit
GGATAAAGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT2920.11197564146319952No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACGGAAGGAAA2910.11159216323901047No Hit
ACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG2900.11120868501482144No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA2890.11082520679063239No Hit
ATCCTAATGTTACCAAATATTTACCATTGGATAAAGGTATTAAACCTTAT2880.11044172856644337No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC2870.11005825034225432No Hit
ATGTGGTATTGGGGGCCAAGACTGTACAGCATCTTGAGTCCCTTTTTACC2850.10929129389387623No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2810.10775738099712008No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC2790.10699042454874201No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT2770.10622346810036391No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG2760.10583998987617488No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA2760.10583998987617488No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT2740.10507303342779681No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC2720.10430607697941874No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG2710.10392259875522969No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCCAAGCAGGCTTTCACTTT2690.1031556423068516No Hit
CTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTC2670.10238868585847354No Hit
GTCGTTGACATTACAGAAAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG2670.10238868585847354No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATA2660.10200520763428449No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACTTAATGACTCTA2620.10047129473752833No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT2620.10047129473752833No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT2620.10047129473752833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)