FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517999
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA30430.5874528715306401No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26970.5206573757864397No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT14950.2886105957733509No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT13950.26930553919988265No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT13890.26814723580547456No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13220.2552128479012508No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT13170.2542475950725773No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGCTGACAGACT12750.24613947131172068No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA12220.23590779132778247No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGGACAAAGACC12110.23378423510470098No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC11240.21698883588578358No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10970.21177647061094712No Hit
CTACAATCCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC10730.20714325703331474No Hit
CCCTTATCCAATGGCAAATATTTGGTGACATTTGGATAAAACCTAGCAGG10600.20463359967876388No Hit
GGGTAAAGGTTCAGGTATTGTTTACATAGAAAGGCCTTGTAAGTTGGCGA10480.20231699288994764No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGAGCGTTCACGGTGGTCTCCATGC10180.19652547591790717No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT10090.194788020826295No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10060.19420886912909097No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGAGCGTTC9870.19054090838013202No Hit
CTTTTACACAATGTGGTTATCCTGCTCTCATGCCTTTGTATGCATGTATT9780.18880345328851986No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTCTGT9490.18320498688221407No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT9460.18262583518501002No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC9440.18223973405354063No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9380.18108143065913254No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTGACATTTGGATAAAAC9220.17799262160737764No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT9090.17548296425282675No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT8930.17239415520107182No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG8880.1714289023723984No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT8430.16274162691433766No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT8240.1590736661653787No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT8220.15868756503390932No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8030.15501960428495037No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGACAACTATTGTGGTTT7880.1521238457989301No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTGACATT7790.15038639070731796No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7710.1488419861814405No Hit
ATCTAGAAGATCTCGTACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7520.14517402543248153No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7410.1430504692094No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7240.1397686095919104No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7180.1386103061975023No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7180.1386103061975023No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT7080.1366798005401555No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA6930.13378404205413524No Hit
TTCTAAAACAGTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGAT6920.13359099148840056No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6870.13262573865972715No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6730.1299230307394416No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC6690.12915082847650283No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACGGAAGGAAA6670.1287647273450335No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6600.1274133733848907No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGATCATTGCCAC6440.12432456433313578No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6380.12316626093872769No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6340.12239405867578894No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6320.12200795754431959No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG6290.12142880584711555No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6270.12104270471564617No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6260.1208496541499115No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG6250.12065660358417679No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGGTCAAAGACC6250.12065660358417679No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6230.12027050245270744No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6140.11853304736109528No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6140.11853304736109528No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6140.11853304736109528No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6020.11621644057227909No Hit
ACAATACGCTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT5970.1152511877436057No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5960.115058137177871No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5870.11332068208625885No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT5850.11293458095478948No Hit
GGATTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5840.11274153038905481No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5840.11274153038905481No Hit
ATCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCAC5810.11216237869185075No Hit
CCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGA5760.11119712586317733No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5690.10984577190303456No Hit
ATGTCATTGGATGTTATGGATCATTGCCACAAGATCACATCATACAGAAA5670.1094596707715652No Hit
GATCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCA5660.10926662020583051No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5650.10907356964009585No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5640.10888051907436114No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC5630.10868746850862647No Hit
ACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGACAA5620.10849441794289179No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA5580.10772221567995305No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA5530.10675696285127963No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTCA5520.10656391228554495No Hit
ATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC5490.1059847605883409No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5420.10463340662819813No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5360.10347510323379004No Hit
GTTATGGATCATTGCCACAAGATCACATCATACAGAAAATCAAAGACTGT5320.10270290097085129No Hit
CACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGT5310.10250985040511662No Hit
GAATACATGCATACAAAGGCATGAGAGCAGGATAACCACATTGTGTAAAA5300.10231679983938194No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5300.10231679983938194No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5280.10193069870791256No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT5270.10173764814217787No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA5260.1015445975764432No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5200.1003862941820351No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)