FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517999
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA27810.5368736233081531No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26780.5169894150374807No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT14030.2708499437257601No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGCTGACAGACT14010.27046384259429074No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA13690.2642862244907809No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT13400.2586877580844751No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT12890.24884217923200622No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT12690.2449811679173126No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11950.23069542605294605No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11900.22973017322427264No Hit
CCCTTATCCAATGGCAAATATTTGGTGACATTTGGATAAAACCTAGCAGG11200.21621663362284485No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGGACAAAGACC11020.21274172343962056No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10750.20752935816478413No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG10440.20154479062700895No Hit
CTTTTACACAATGTGGTTATCCTGCTCTCATGCCTTTGTATGCATGTATT10150.19594632422070313No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTGACATTTGGATAAAAC10130.19556022308923376No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10050.19401581856335628No Hit
CTACAATCCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC9920.19150616120880543No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9620.18571464423676493No Hit
GGGTAAAGGTTCAGGTATTGTTTACATAGAAAGGCCTTGTAAGTTGGCGA9190.17741346991017357No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTCTGT8880.1714289023723984No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8870.1712358518066637No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT8750.16891924501784752No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC8670.16737484049197007No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8590.1658304359660926No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8450.16312772804580705No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT8400.16216247521713362No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7900.1525099469303995No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7830.15115859297025672No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT7810.15077249183878733No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTGACATT7690.14845588504997115No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGAGCGTTC7670.14806978391850176No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7550.14575317712968558No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGAGCGTTCACGGTGGTCTCCATGC7340.14169911524925724No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC7140.13783810393456358No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7140.13783810393456358No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA7070.13648674997442078No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGATCATTGCCAC6990.13494234544854333No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6810.13146743526531907No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6600.1274133733848907No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6560.12664117112195197No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6540.1262550699904826No Hit
TTCTAAAACAGTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGAT6460.12471066546460513No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6420.1239384632016664No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6420.1239384632016664No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6380.12316626093872769No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6280.12123575528138085No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6280.12123575528138085No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6250.12065660358417679No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG6170.11911219905829933No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA6090.11756779453242189No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG6020.11621644057227909No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGACAACTATTGTGGTTT6000.11583033944080974No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5950.11486508661213632No Hit
ATCTAGAAGATCTCGTACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5920.11428593491493227No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5920.11428593491493227No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC5860.11312763152052417No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5830.11254847982332013No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5830.11254847982332013No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGGTCAAAGACC5780.1115832269946467No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5750.11100407529744266No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5740.11081102473170798No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5640.10888051907436114No Hit
GGATTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5630.10868746850862647No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA5590.10791526624568774No Hit
CACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGT5590.10791526624568774No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA5530.10675696285127963No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5510.10637086171981028No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG5420.10463340662819813No Hit
CCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGA5410.10444035606246344No Hit
GTTATGGATCATTGCCACAAGATCACATCATACAGAAAATCAAAGACTGT5390.10405425493099409No Hit
ATCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCAC5390.10405425493099409No Hit
ATGTCATTGGATGTTATGGATCATTGCCACAAGATCACATCATACAGAAA5310.10250985040511662No Hit
GTATGATGTGATCTTGTGGCAATGATCCATAACATCCAATGACATAGCCC5290.10212374927364724No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5250.10135154701070852No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT5210.10057934474776978No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC5200.1003862941820351No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)