FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472352
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20590.43590373280943023No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA19550.4138862543188131No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA14640.30993835106022627No Hit
CACTCATATGGGCCTAAAGTTCAGGCAACTTTTATGGTTTCACATTTCTT12370.2618809701239754No Hit
GTGTAAAAGGGGCAGCGAAACCCAAAAGACCCACAATACGTTGACAGACT11380.24092202425309936No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCATA11100.23499424158254864No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA10830.22927816543594606No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT9880.20916604566086305No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA9570.20260314341846758No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT9560.20239143689451933No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACCATTTAAATTTCAT8750.18524320845471173No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8580.18164419754759162No Hit
GTTGACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTCTGATGTGAT8470.179315425784161No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA8430.178468599688368No Hit
CCTCCAGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA8100.171482284398076No Hit
GGGGATACCATTTAAATTTCATGGGCTATGTCATTGGAAGTTATGGATCA7640.1617437842964569No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7640.1617437842964569No Hit
GACTTGTGTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT7410.15687453424564732No Hit
GAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGC7290.1543340559582684No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7280.15412234943432018No Hit
GACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT7270.15391064291037193No Hit
GTACAAGATCGTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGA6700.14184337104532213No Hit
CTATGTATCCCTACTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6660.14099654494952918No Hit
GTCCAAGAGTCCTCTTATGTAAGACAGAGAGTCCAAGAGTCCTCTTATGT6630.14036142537768445No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6630.14036142537768445No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT6600.13972630580583972No Hit
GGGTTACCATTTAAATTTCATGGGCTATGTCATTGGAAGTTATGGATCAT6580.13930289275794322No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6570.13909118623399497No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6560.13887947971004674No Hit
GTGTGGTGAGGTGAACAATGCGCAGGAGACTCTAAGGCTTCCCGATAGAG6540.13845606666215024No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTTTTATGGTTTCACATTTCTT6480.1371858275184608No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6470.13697412099451256No Hit
ATTTTGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACA6310.1335868166113407No Hit
GGTAACCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGAC6180.13083463180001356No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6160.1304112187521171No Hit
TCTTAAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTA6080.12871756656053113No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6070.12850586003658288No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5980.12660050132104872No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5910.125118555653411No Hit
TGGTATCCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGA5800.12278978388998035No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT5700.12067271865049793No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5560.11770882731522254No Hit
CTAATTTGGGCCTAAAGTTCAGGCAACTTTTATGGTTTCACATTTCTTGT5530.11707370774337782No Hit
ATATGAGTGTTGACATAACTAACTACTAGGTCCCTAGATGCTGGATCTTC5350.11326299031230945No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAGTACAATTAAT5280.11178104464467176No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATTCATTTACA5280.11178104464467176No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC5090.10775862068965517No Hit
TAACCACATTGTGTAAAAGGGGCAGCGAAACCCAAAAGACCCACAATACG5080.10754691416570693No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5070.10733520764175869No Hit
GTTTTCTAAAACATTCTTTGATTTTTTGTCTGATGTGATCTTGTGGCAAT5060.10712350111781045No Hit
GGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAACATACCTTGAT5050.1069117945938622No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGATACCATTTAAATTTCAT5040.10670008806991398No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA5040.10670008806991398No Hit
CCTTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTTTCA4990.10564155545017276No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4970.10521814240227628No Hit
GGAACACACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGAACTAATGAC4910.10394790325858681No Hit
ATATTAGTGTTGACATAACTAACTACTAGGTCCCTAGATGCTGGATCTTC4890.10352449021069034No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTCTTACATAAGAGGACTCT4850.10267766411489736No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4790.10140742497120792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)