FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472352
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN76981.6297168213535669No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA18360.3886931779689723No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA14040.29723595962333177No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCATA11790.2496019917349773No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA10810.2288547523880496No Hit
CACTCATATGGGCCTAAAGTTCAGGCAACTTTTATGGTTTCACATTTCTT10630.22504403495698122No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA9330.19752218684370978No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT9280.19646365422396855No Hit
GTGTAAAAGGGGCAGCGAAACCCAAAAGACCCACAATACGTTGACAGACT8790.1860900345505047No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA8570.1814324910236434No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACCATTTAAATTTCAT8350.17677494749678205No Hit
GTTGACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTCTGATGTGAT8120.1719056974459725No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7910.1674598604430594No Hit
CCTCCAGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA7820.16555450172752526No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7180.15200528419483775No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT7100.15031163200325182No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7080.1498882189553553No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6980.1477711537158729No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6790.14374872976085631No Hit
GGGGATACCATTTAAATTTCATGGGCTATGTCATTGGAAGTTATGGATCA6740.14269019714111508No Hit
GGGTTACCATTTAAATTTCATGGGCTATGTCATTGGAAGTTATGGATCAT6650.14078483842558093No Hit
CCTGTCAACAGGCCAATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT6630.14036142537768445No Hit
GACTTGTGTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT6600.13972630580583972No Hit
GTACAAGATCGTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGA6040.12787074046473815No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6010.12723562089289345No Hit
GTTTTCTAAAACATTCTTTGATTTTTTGTCTGATGTGATCTTGTGGCAAT6000.1270239143689452No Hit
GACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT5930.1255419687013075No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTTTTATGGTTTCACATTTCTT5850.12384831650972157No Hit
TCTTAAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTA5810.1230014904139286No Hit
CTATGTATCCCTACTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5750.12173125127023915No Hit
GGTAACCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGAC5700.12067271865049793No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5690.12046101212654968No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5680.12024930560260146No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5630.11919077298286024No Hit
GAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGC5430.1149566425038954No Hit
GTGTGGTGAGGTGAACAATGCGCAGGAGACTCTAAGGCTTCCCGATAGAG5400.11432152293205068No Hit
CCTTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTTTCA5380.1138981098841542No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5300.11220445769256826No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT5290.11199275116862001No Hit
ATTTTGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACA5270.11156933812072353No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAGTACAATTAAT5170.10945227288124111No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5160.10924056635729287No Hit
ATATTAGTGTTGACATAACTAACTACTAGGTCCCTAGATGCTGGATCTTC5090.10775862068965517No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC5080.10754691416570693No Hit
TGGTATCCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGA5050.1069117945938622No Hit
GTCCAAGAGTCCTCTTATGTAAGACAGAGAGTCCAAGAGTCCTCTTATGT5040.10670008806991398No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATTCATTTACA4960.10500643587832803No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA4930.1043713163064833No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4880.10331278368674209No Hit
CTAATTTGGGCCTAAAGTTCAGGCAACTTTTATGGTTTCACATTTCTTGT4790.10140742497120792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)