FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536965
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43300.8063840287542019No Hit
TCCATAGAGTGTGTAAATAATGTCTTGTTTGGAAGTAATGATTAACTAGA26050.48513404039369423No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC13310.2478746287001946No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11070.2061586881826562No Hit
GGATAAGGGTATTAAACCTTATTATCCCGAACATCTAGTTAATCATTACT9990.1860456454331288No Hit
CATTTACATCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9740.1813898485003678No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAATAGA9200.1713333271256041No Hit
ATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA9090.16928477647518925No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT8610.16034564636428816No Hit
CATCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT8150.15177898000800796No Hit
GTATTCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA8140.1515927481306975No Hit
CATGAATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC7620.14190869051055469No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC6690.12458912592068383No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6580.122540575270269No Hit
GTATTAAACCTTATTATCCCGAACATCTAGTTAATCATTACTTCCAAACA6560.12216811151564815No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6500.1210507202517855No Hit
GTGTAAATAATGTCTTGTTTGGAAGTAATGATTAACTAGATGTTCGGGAT6490.12086448837447505No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6420.11956086523330198No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6380.11881593772406022No Hit
ACCATTTACATTTCATGGGCTATGTTATTGGATGTTATGGGTCATTGCCA6300.11732608270557672No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6260.11658115519633495No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6130.11416014079129926No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6030.11229782201819485No Hit
AATATGGGCCTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCTTGTCT6000.11173912638626354No Hit
GTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG5900.10987680761315914No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT5870.10931811198122783No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5690.10596593818963992No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTTGTTTGGAAGT5670.10559347443501903No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT5660.1054072425577086No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5630.10484854692577729No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5590.10410361941653552No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5590.10410361941653552No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5550.10335869190729377No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC5540.10317246002998334No Hit
GCCTTAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC5470.10186883688881027No Hit
GTACAACATCTTGAGTCCATTTTTACCGCTGTTACCAATTTTCTTTTGTC5470.10186883688881027No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGGCCCACAATACGTTGACAGACT5460.10168260501149982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)