FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422053
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19070.45183898704665054No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17820.4222218536534511No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17210.4077686925575698No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT15940.37767768503007915No Hit
TAAATGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATCATC14620.3464019921668606No Hit
ACTCACAACTGTGCCAACAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13760.32602540439233935No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC13210.31299386569933163No Hit
GTTGTGAGTATGCCCTGAGCTTGAGGGCTCCACCCCAACAGTCCCCCATG12750.3020947606106342No Hit
ATCCCAGAGTCAGGGCTCTGTACTTTCCTGCTGGTGGCTCCAGTTCAGGA12430.29451277446197516No Hit
CTCTTGGATCTTGAAGAGTTTGGTGGAACGTGGTGGAGTTCCACTGCATT12170.28835241071618967No Hit
GGTTTAGGTCCCAATCGGGATTGTCGGAGTTGGCTTTGAATGCAGGGTCC11310.2679758229416685No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC10460.24783617223429286No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA10420.24688842396571048No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT10260.24309743089138092No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG10140.2402541860856338No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATACCCACATTGTGTGAAA9050.2144280457667639No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8910.21111092682672555No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG8760.20755687081954163No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT8630.20447668894664886No Hit
ATTTTGTAACATTGGGATAGAACCTAGCAGGCATAATCAATTTTAGTCTC8310.19689470279798982No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8280.19618389159655306No Hit
CTGTAAGGGGTCCCACAAATTGTTTACACCTATTAATGATGTCCTCCTGC8060.19097127611934994No Hit
CCCTTAACTTCATGGGGTATGTAATTGGAAGTTGGGGCACCTTACCCCAA7590.17983523396350695No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7260.1720163107477023No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAAAGGACGACAGAAT7160.16964694007624634No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACGTATAAGGCC7120.168699191807664No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7110.16846225474051837No Hit
GGGTTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGAC6910.16372351339760646No Hit
GTATAAGGCCTTTCTAAACAAACAATATCTGAACCTTTACCCCGTTGCTC6900.16348657633046088No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6830.1618280168604417No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6820.1615910797932961No Hit
CCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGAAAC6780.16064333152471372No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6670.15803702378611217No Hit
GTTCTGAACAGGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6520.15448296777892823No Hit
ACCTCAACCCACACAAGGACAACTGGCCGGACTCCAACAAGGTGGGAGTG6500.15400909364463705No Hit
TACCCACATTGTGTGAAAGGAGCAGCAAATCCCAAAAGACCCACAATTCG6440.15258747124176347No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6410.1518766600403267No Hit
ACAAAACGGTGGGGCTACTCCCTTAACTTCATGGGGTATGTAATTGGAAG6380.15116584883888992No Hit
GTTCAGAACACTGCCTCTTCCATATCGTCAATCTTATCGAAGACTGGGGA6370.1509289117717443No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT6310.14950728936887073No Hit
GCCTTATACGTTGGCGAGAAAGTAAAAGCCTGTTTAGCTTGTATACATGC6240.14784872989885156No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT6220.1473748557645604No Hit
CTCTGGGATCTTGAAGAGTTTGGTGGAACGTGGTGGAGTTCCACTGCATT6210.14713791869741477No Hit
ATCCAAGAGTCAGGGCTCTGTACTTTCCTGCTGGTGGCTCCAGTTCAGGA6190.1466640445631236No Hit
ATATGCAGGAGGTGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6190.1466640445631236No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT6110.14476854802595882No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG6100.14453161095881323No Hit
TCCTCAGGCAATGCAGTGGAACTCCACCACGTTCCACCAAACTCTTCAAG6060.14358386269023085No Hit
CCTATTAATGATGTCCTCCTGCAAATGAATGTCAGGAAAGGAAGGAGTTT6040.14310998855593965No Hit
CAATATGTTCTTGGGGTAAGGTGCCCCAACTTCCAATTACATACCCCATG6020.14263611442164845No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA5940.14074061788448372No Hit
GTCCCTTTATGCCGCTGTTACCAATTTTCTTTTATCTTTGGGCATACATT5920.14026674375019252No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5880.13931899548161014No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5810.13766043601159095No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC5750.1362388136087174No Hit
GTGTGAAAGGAGCAGCAAATCCCAAAAGACCCACAATTCGTTGACATACT5730.1357649394744262No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5720.1355280024072806No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5710.13529106534013502No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5670.13434331707155264No Hit
GGGTAAAGGTTCAGATATTGTTTGTTTAGAAAGGCCTTATACGTTGGCGA5590.13244782053438786No Hit
CCCTTACAGTGAATGAAAACAGGAGACTAAAATTGATTATGCCTGCTAGG5410.12818295332576715No Hit
GTTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTCAACACAATATGTT5390.12770907919147595No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG5360.12699826799003916No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACACGTAGCGCC5350.12676133092289357No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5200.12320727491570965No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5190.12297033784856404No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT5170.12249646371427286No Hit
GGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCT5130.12154871544569046No Hit
ATGTGGGTATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC5100.12083790424425368No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA5080.1203640301099625No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTCAAC5060.1198901559756713No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5050.11965321890852572No Hit
TTACTAAATATTTGCCCTTAGATAAAGGAATTAAACCTTATTATCCAGAG5010.11870547063994331No Hit
CTTTTGGAGTGTGGATTCGCACACCTCCTGCATATAGACCACAAAATGCC5010.11870547063994331No Hit
ATGTATATTTGCGGGAAAGGACGACAGAATTGTCCGTCCCGATAAGTTTT4950.11728384823706975No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4910.11633609996848737No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAGTCTCCTGTTTTCATTCAC4890.11586222583419617No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4890.11586222583419617No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4850.1149144775656138No Hit
GTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACACG4730.11207123275986666No Hit
TCATTAATAGGTGTAAACAATTTGTGGGACCCCTTACAGTGAATGAAAAC4720.11183429569272105No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4720.11183429569272105No Hit
ACTCACCCCAACACAGAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAA4710.11159735862557546No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4670.11064961035699308No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4500.10662168021551796No Hit
CCCCAACAGTCCCCCATGGGGTGGAGTGAATCCCGGCCCGAATGCTCCCA4410.10448924661120759No Hit
GGCCTGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTTGTTT4400.104252309544062No Hit
GTACAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG4360.10330456127547961No Hit
GTTTACACCTATTAATGATGTCCTCCTGCAAATGAATGTCAGGAAAGGAA4360.10330456127547961No Hit
GGTTGAGGTCCCAATCGGGATTGTCGGAGTTGGCTTTGAATGCAGGGTCC4320.10235681300689724No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG4310.10211987593975165No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGAGCCCT4280.10140906473831486No Hit
GATGCGATGTTCTCCATGTTCGGCACAGGGTCCCCAGTCTTCGATAAGAT4280.10140906473831486No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCAGA4280.10140906473831486No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGATTT4260.10093519060402367No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)