FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422053
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18960.449232679308049No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT14970.3546947895169564No Hit
ACTCACAACTGTGCCAACAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT14940.3539839783155196No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13540.32081278891513626No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13040.30896593555785645No Hit
TAAATGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATCATC12400.29380196326053837No Hit
ATCCCAGAGTCAGGGCTCTGTACTTTCCTGCTGGTGGCTCCAGTTCAGGA12400.29380196326053837No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC11580.27437312375459955No Hit
GTTGTGAGTATGCCCTGAGCTTGAGGGCTCCACCCCAACAGTCCCCCATG11180.2648956410687757No Hit
CTCTTGGATCTTGAAGAGTTTGGTGGAACGTGGTGGAGTTCCACTGCATT10790.2556550954500975No Hit
GGTTTAGGTCCCAATCGGGATTGTCGGAGTTGGCTTTGAATGCAGGGTCC9890.23433075940699394No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG9550.22627489912404367No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT8950.21205867509530796No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATACCCACATTGTGTGAAA8680.20566137428237688No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8420.19950101053659136No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8160.1933406467908059No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG8140.1928667726565147No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8100.19191902438793232No Hit
ATTTTGTAACATTGGGATAGAACCTAGCAGGCATAATCAATTTTAGTCTC7760.18386316410498207No Hit
CCCTTAACTTCATGGGGTATGTAATTGGAAGTTGGGGCACCTTACCCCAA7700.1824415417021085No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT7550.17888748569492458No Hit
GTTCAGAACACTGCCTCTTCCATATCGTCAATCTTATCGAAGACTGGGGA7290.17272712194913908No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7240.17154243661341112No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7170.16988387714339193No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6870.16277576512902409No Hit
GTATAAGGCCTTTCTAAACAAACAATATCTGAACCTTTACCCCGTTGCTC6870.16277576512902409No Hit
CTGTAAGGGGTCCCACAAATTGTTTACACCTATTAATGATGTCCTCCTGC6800.16111720565900492No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACGTATAAGGCC6590.15614152724894742No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6560.15543071604751063No Hit
ATCCAAGAGTCAGGGCTCTGTACTTTCCTGCTGGTGGCTCCAGTTCAGGA6530.15471990484607384No Hit
CTCTGGGATCTTGAAGAGTTTGGTGGAACGTGGTGGAGTTCCACTGCATT6470.15329828244320026No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6430.15235053417461789No Hit
ATATGCAGGAGGTGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6420.1521135971074723No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC6330.14998116350316193No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAAAGGACGACAGAAT6270.14855954110028835No Hit
CCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGAAAC6210.14713791869741477No Hit
ACCTCAACCCACACAAGGACAACTGGCCGGACTCCAACAAGGTGGGAGTG6070.14382079975737644No Hit
TACCCACATTGTGTGAAAGGAGCAGCAAATCCCAAAAGACCCACAATTCG6050.14334692562308524No Hit
GGGCTGTACCGCCCGCTTCTCCGTCTGCCGTACCGACCGACCACGGGGCG5860.13884512134731894No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5830.13813431014588215No Hit
GGGTTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGAC5680.13458025413869823No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT5670.13434331707155264No Hit
GTTCTGAACAGGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5650.13386944293726144No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT5640.13363250587011585No Hit
CCTATTAATGATGTCCTCCTGCAAATGAATGTCAGGAAAGGAAGGAGTTT5610.13292169466867906No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5540.13126313519865987No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5530.13102619813151428No Hit
GTGTGAAAGGAGCAGCAAATCCCAAAAGACCCACAATTCGTTGACATACT5420.12841989039291274No Hit
GTACAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG5410.12818295332576715No Hit
GGGTAAAGGTTCAGATATTGTTTGTTTAGAAAGGCCTTATACGTTGGCGA5390.12770907919147595No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5360.12699826799003916No Hit
TCCTCAGGCAATGCAGTGGAACTCCACCACGTTCCACCAAACTCTTCAAG5350.12676133092289357No Hit
ACAAAACGGTGGGGCTACTCCCTTAACTTCATGGGGTATGTAATTGGAAG5330.12628745678860237No Hit
GTCCCTTTATGCCGCTGTTACCAATTTTCTTTTATCTTTGGGCATACATT5330.12628745678860237No Hit
CAATATGTTCTTGGGGTAAGGTGCCCCAACTTCCAATTACATACCCCATG5310.12581358265431117No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5260.12462889731858322No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA5170.12249646371427286No Hit
GCCTTATACGTTGGCGAGAAAGTAAAAGCCTGTTTAGCTTGTATACATGC5130.12154871544569046No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5070.1201270930428169No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACACGTAGCGCC5050.11965321890852572No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG4990.11823159650565214No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4970.11775772237136095No Hit
GGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCT4900.11609916290134178No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA4890.11586222583419617No Hit
CCCTTACAGTGAATGAAAACAGGAGACTAAAATTGATTATGCCTGCTAGG4810.1139667292970314No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG4810.1139667292970314No Hit
GTTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTCAACACAATATGTT4770.11301898102844903No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTCAAC4750.11254510689415784No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4720.11183429569272105No Hit
ATGTGGGTATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC4720.11183429569272105No Hit
TCATTAATAGGTGTAAACAATTTGTGGGACCCCTTACAGTGAATGAAAAC4710.11159735862557546No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA4700.11136042155842987No Hit
GGCCTGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTTGTTT4690.11112348449128426No Hit
CTTTTGGAGTGTGGATTCGCACACCTCCTGCATATAGACCACAAAATGCC4670.11064961035699308No Hit
CCCCAACAGTCCCCCATGGGGTGGAGTGAATCCCGGCCCGAATGCTCCCA4640.1099387991555563No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4630.10970186208841069No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4550.10780636555124594No Hit
ATGTATATTTGCGGGAAAGGACGACAGAATTGTCCGTCCCGATAAGTTTT4500.10662168021551796No Hit
CAGTAAACCCTGTTCAGAACACTGCCTCTTCCATATCGTCAATCTTATCG4480.10614780608122676No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4470.10591086901408117No Hit
TTACTAAATATTTGCCCTTAGATAAAGGAATTAAACCTTATTATCCAGAG4460.10567393194693558No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4460.10567393194693558No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCG4460.10567393194693558No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAGTCTCCTGTTTTCATTCAC4450.10543699487978997No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGATTT4440.10520005781264438No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4440.10520005781264438No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCAGA4410.10448924661120759No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGCCTGTGCCAAGTGTTTGCTG4390.1040153724769164No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4360.10330456127547961No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG4360.10330456127547961No Hit
GTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACACG4350.10306762420833403No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAT4310.10211987593975165No Hit
GTTTACACCTATTAATGATGTCCTCCTGCAAATGAATGTCAGGAAAGGAA4290.10164600180546045No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGATCA4280.10140906473831486No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4270.10117212767116926No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4260.10093519060402367No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)