FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380394
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC21950.5770332865397456No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA16120.4237711425521959No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15540.40852379375069003No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12580.3307097377981777No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9310.24474623679658458No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9300.24448335147242067No Hit
GAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTATTGATT8880.2334421678575372No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7910.20794229141363954No Hit
GTCGAATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA7900.20767940608947566No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC7900.20767940608947566No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7300.19190628663964204No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG7200.18927743339800313No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG6910.18165375899725023No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6790.1784991351072835No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG6730.17692182316230015No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6660.17508162589315288No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6540.17192700200318617No Hit
GTCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGA6510.17113834603069447No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGA6470.17008680473403892No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6320.16614352487158052No Hit
GCATTCGACAAGGCATGGGGACAAATCTTTCTGTTCCCAATCCTCTGGGA6100.16036004773997486No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6000.15773119449833595No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5970.15694253852584425No Hit
CTTGATTTTCAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAA5890.15483945593253312No Hit
ATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT5880.1545765706083692No Hit
CTTGTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG5830.15326214398754975No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5790.15221060269089418No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5770.1516848320425664No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5720.15037040542174693No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5660.14879309347676356No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5620.14774155218010798No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5560.14616424023512462No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5550.14590135491096076No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5550.14590135491096076No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5500.14458692829014128No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5440.1430096163451579No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5430.14274673102099403No Hit
GGTCCGGTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGC5360.14090653375184678No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACAAATCTT5310.1395921071310273No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5220.13722613921355226No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5210.13696325388938838No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5130.13486017129607722No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5090.13380862999942167No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5000.13144266208194663No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4830.1269736115711604No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTAAGGAAAGGAGGGAGTTTG4770.12539629962617707No Hit
ACACAAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA4760.12513341430201316No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4680.12303033170870203No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC4670.12276744638453814No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4630.12171590508788256No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT4610.12119013443955477No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4590.120664363791227No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4570.1201385931428992No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTC4510.11856128119791585No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4500.11829839587375196No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4490.11803551054958807No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4470.11750973990126026No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4330.11382934536296575No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4300.11304068939047408No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4290.11277780406631019No Hit
ACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA4280.11251491874214631No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4270.11225203341798241No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAACGTAAGA4270.11225203341798241No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC4230.11120049212132684No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG4200.11041183614883515No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4180.10988606550050738No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4140.1088345242038518No Hit
CAACACAAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTG4110.10804586823136011No Hit
CTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTTAC4080.10725721225886843No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT4080.10725721225886843No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA4050.10646855628637676No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG4020.10567990031388506No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGG3970.1043654736930656No Hit
TACACACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGC3920.10305104707224616No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3910.10278816174808225No Hit
GTTCTATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAAC3890.10226239109975446No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA3890.10226239109975446No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG3890.10226239109975446No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC3880.10199950577559057No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3870.10173662045142669No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGAGATCTCCTCGACACC3860.10147373512726279No Hit
CAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTG3830.10068507915477112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)