FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506322
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC21870.4319385687368908No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG18550.36636764746544687No Hit
ACTCACAACTGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT17860.35273995599638175No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG17480.34523485054965025No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC16540.3266695897077354No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG16310.3221270258847137No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTTGT16030.31659694818712203No Hit
ACCTCATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTG14340.2832189792266582No Hit
GTTCCGCTCACCATACGGCACTCAGGCAAGCTATTCTATGTTGGGGTGAG14080.2780839070788944No Hit
GGACGACCCCTGGCCCGAATGCTCCCACTCCCACCTTGTTGGCGTCCGGC13840.2733438404809588No Hit
ATACAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT13430.2652462267094853No Hit
ATATGGAGGAGGAGTGCGAATCCACACTCCAAAGGACACCAAATATTCAA13250.2616911767610335No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACAAGACATTATTTACAC12600.2488534963916243No Hit
GGCGCTGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGG11670.23048573832462346No Hit
GAATCAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC11220.221598113453494No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA11030.21784556073012828No Hit
GTTCACCTCACCATACGGCACTCAGGCAAGCTATTCTATGTTGGGGTGAG10470.20678540533494494No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCACG10310.20362536093632114No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA10190.20125532763735332No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT10070.19888529433838548No Hit
GGGTTCATCCCTCCCCATGGGGGACTGTTGGGGTGGAGCCCTCAGGCTCA9930.1961202554895896No Hit
ACGTCAATATGGGCATAAAACTCAGACAATTATTGTGGTTTCACATTTCC9800.19355271941570779No Hit
GCTCCGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA9710.1917751944414819No Hit
GAAACTGCACCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATAC9580.18920765836760006No Hit
GTCCACGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC9380.1852576028693203No Hit
GGCCGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCAC9290.18348007789509443No Hit
TATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTCC9290.18348007789509443No Hit
CTCTAATTCCAGGATCATCAACAACCAGCACCGGACCATGCAAAACCTGC9250.18269006679543848No Hit
GGAGTCGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGG9030.17834500574733075No Hit
GCCTAATCATCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8960.17696248632293282No Hit
CCTCCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8760.1730124308246531No Hit
CTCCTGAACATTGTTCACCTCACCATACGGCACTCAGGCAAGCTATTCTA8750.17281492804973908No Hit
CCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC8580.1694573808762013No Hit
GCACCGGACCATGCAAAACCTGCACAGCGCCTGCTCAAGGAACCTCTATG8460.16708734757723345No Hit
CCTTTGGAGTGTGGATTCGCACTCCTCCTCCATATAGACCACCAAATGCC8340.1647173142782656No Hit
GCACAGCGCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA8270.1633347948538677No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA8220.16234728097929776No Hit
CTTTTGTATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTTGT8220.16234728097929776No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8130.16056975600507187No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT8130.16056975600507187No Hit
CTCCAAAGGACACCAAATATTCAAGAACAACTTCTCTCCCAAAAGTAAGA8110.1601747504552439No Hit
ATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTTGCC8060.15918723658067396No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7870.1554346838573082No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCCGAAAGTCCAA7860.1552371810823942No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7850.15503967830748022No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7660.15128712558411445No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAGGTAA7600.15010210893463052No Hit
TGCTTGATCTTCCAAATTACCTCCCACCCAGGTGGCTAGATTCATCAACT7560.14931209783497457No Hit
ATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTG7510.14832458396040465No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT7470.1475345728607487No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7400.1461520534363508No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA7340.14496703678686687No Hit
ACCTAATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTG7320.14457203123703888No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7180.14180699238824304No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA7140.1410169812885871No Hit
TCCAAATTACCTCCCACCCAGGTGGCTAGATTCATCAACTCACCCCAACA7130.14081947851367313No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7120.14062197573875912No Hit
TCCAAAGAGTGTGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACA7100.14022697018893115No Hit
TCCTTGGACACATAAGGTGGGAGACTTTACGGGGCTTTATTCTTCTACGG6850.13528940081608146No Hit
CATCCTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG6800.13430188694151152No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6800.13430188694151152No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6660.1315368480927157No Hit
TATACAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATAT6620.13074683699305975No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC6510.1285743064690059No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6500.1283768036940919No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG6500.1283768036940919No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6480.12798179814426394No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTACGGAGTTACTCTCTTTT6480.12798179814426394No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6440.12719178704460798No Hit
ATGTCAGGAAAAGAAGGAGTTTGCCAATTAGGATTAAAGCAAGGTACCGT6420.12679678149478No Hit
ACATCGACCCGTATAAAGAATTTGGAGCTTCTACGGAGTTACTCTCTTTT6390.12620427317003802No Hit
TATTAACGTTGACATAGCCGACTACTAAATCCCTGGATGCTTGATCTTCC6340.1252167592954681No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT6300.12442674819581215No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6300.12442674819581215No Hit
GTTTCAGGCCCATATTAACGTTGACATAGCCGACTACTAATTCCCTGGAT6270.1238342398710702No Hit
ATATGTTCTTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA6240.12324173154632823No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6180.12205671489684429No Hit
ATCTCATCGAAGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATC6180.12205671489684429No Hit
GTCTCTGCCATATCGTCAATCTCATCGAAGACTGGGGACCCTGTACCGAA6140.12126670379718835No Hit
GGGACGACCCCTGGCCCGAATGCTCCCACTCCCACCTTGTTGGCGTCCGG6110.12067419547244639No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6100.12047669269753242No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6010.1186991677233065No Hit
AGTATACATCGTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG6010.1186991677233065No Hit
ATCATCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGG6000.11850166494839252No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6000.11850166494839252No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAATCACTCACC5980.11810665939856456No Hit
CAATTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAGTTGT5970.11790915662365055No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5930.1171191455239946No Hit
GGAACGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG5920.11692164274908062No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5860.1157366260995967No Hit
GGCAGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCAC5660.11178657060131696No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5650.11158906782640296No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG5640.11139156505148898No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5600.11060155395183303No Hit
GATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCC5550.10961404007726308No Hit
GACCTGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCAGTATGGATCGG5550.10961404007726308No Hit
GTTTCGCTCCAGACCTGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA5540.1094165373023491No Hit
GGGCAAAACTCATCGGGACTGACAATTCTGTCGTGCTCTCCCGCAAGTAT5520.10902153175252113No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTTT5520.10902153175252113No Hit
ATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCA5480.10823152065286518No Hit
GTTCCGGAGACTCTAAGGCCTCCCGATACAGAGCAGAGGCAGTGTCGAGG5470.10803401787795118No Hit
GATGCAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAACCAGGAGACTA5410.10684900122846727No Hit
CATTATTGACAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAA5400.10665149845355328No Hit
GGGAATTAGTAGTCGGCTATGTCAACGTTAATATGGGCCTAAAACTCAGA5350.10566398457898334No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT5350.10566398457898334No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5340.10546648180406934No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCCGCTCACCATACGG5330.10526897902915537No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTTAGGAAAAGAA5290.10447896792949941No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5280.10428146515458542No Hit
CCCCAACAGTCCCCCATGGGGAGGGACGACCCCTGGCCCGAATGCTCCCA5280.10428146515458542No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5270.10408396237967144No Hit
GGTCTATATGGAGGAGGAGTGCGAATCCACACTCCAAAGGACACCAAATA5240.10349145405492946No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTACGG5210.10289894573018751No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGAAGGGGC5200.10270144295527353No Hit
GTGCAATTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGAAACATAGA5150.10171392908070358No Hit
GTAATCTGGAAGATCCAGCATCCAGGGAATTAGTAGTCGGCTATGTCAAC5140.10151642630578961No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5100.10072641520613365No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGTGAATCCAGATTGGGACCT5070.10013390688139168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)