FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506322
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG19440.3839453944327918No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC18140.35827003369397337No Hit
ACTCACAACTGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT16600.32785460635721936No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG16130.3185719759362619No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG15890.3138319093383262No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTTGT15640.30889433996547655No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC15300.3021792456184009No Hit
GTTCCGCTCACCATACGGCACTCAGGCAAGCTATTCTATGTTGGGGTGAG14050.2774913987541525No Hit
ATACAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT12710.25102602691567816No Hit
ATATGGAGGAGGAGTGCGAATCCACACTCCAAAGGACACCAAATATTCAA12350.2439159270187746No Hit
GGACGACCCCTGGCCCGAATGCTCCCACTCCCACCTTGTTGGCGTCCGGC11970.23641082157204307No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACAAGACATTATTTACAC11650.23009073277479547No Hit
GGCGCTGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGG11020.21764805795521425No Hit
GTCCACGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC10630.20994544973356877No Hit
GTTCACCTCACCATACGGCACTCAGGCAAGCTATTCTATGTTGGGGTGAG10590.2091554386339128No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA10580.20895793585899883No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT10420.20579789146037503No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCACG10260.2026378470617512No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA10240.20224284151192326No Hit
CTCTAATTCCAGGATCATCAACAACCAGCACCGGACCATGCAAAACCTGC9690.19138018889165392No Hit
GAAACTGCACCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATAC9380.1852576028693203No Hit
GCTCCGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA9350.18466509454457836No Hit
CTCCTGAACATTGTTCACCTCACCATACGGCACTCAGGCAAGCTATTCTA9330.1842700889947504No Hit
GGCCGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCAC9280.18328257512018045No Hit
GAATCAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC8990.1775549946476748No Hit
CTCCAAAGGACACCAAATATTCAAGAACAACTTCTCTCCCAAAAGTAAGA8920.1761724752232769No Hit
CCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC8820.174197447474137No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT8780.17340743637448108No Hit
ACGTCAATATGGGCATAAAACTCAGACAATTATTGTGGTTTCACATTTCC8530.16846986700163138No Hit
TATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTCC8490.16767985590197543No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT8470.16728485035214746No Hit
GGGTTCATCCCTCCCCATGGGGGACTGTTGGGGTGGAGCCCTCAGGCTCA8450.16688984480231947No Hit
CTTTTGTATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTTGT8160.16116226432981384No Hit
CCTCCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8160.16116226432981384No Hit
GCCTAATCATCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8130.16056975600507187No Hit
CATCCTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG8090.15977974490541594No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8070.15938473935558795No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA7990.15780471715627606No Hit
GGAGTCGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGG7950.1570147060566201No Hit
CCTTTGGAGTGTGGATTCGCACTCCTCCTCCATATAGACCACCAAATGCC7950.1570147060566201No Hit
GCACCGGACCATGCAAAACCTGCACAGCGCCTGCTCAAGGAACCTCTATG7800.15405216443291028No Hit
GCACAGCGCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA7800.15405216443291028No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7650.15108962280920046No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA7590.14990460615971654No Hit
ATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTTGCC7500.14812708118549064No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7440.14694206453600675No Hit
TGCTTGATCTTCCAAATTACCTCCCACCCAGGTGGCTAGATTCATCAACT7440.14694206453600675No Hit
ATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTG7430.14674456176109274No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCCGAAAGTCCAA7320.14457203123703888No Hit
TCCAAAGAGTGTGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACA7300.1441770256872109No Hit
GTTTCAGGCCCATATTAACGTTGACATAGCCGACTACTAATTCCCTGGAT7270.14358451736246894No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAGGTAA7230.142794506262813No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7160.14141198683841508No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT7070.1396344618641892No Hit
TCCTTGGACACATAAGGTGGGAGACTTTACGGGGCTTTATTCTTCTACGG6870.13568440636590942No Hit
AGTATACATCGTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG6780.13390688139168355No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6690.13212935641745768No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC6680.13193185364254367No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6660.1315368480927157No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6660.1315368480927157No Hit
GTCTCTGCCATATCGTCAATCTCATCGAAGACTGGGGACCCTGTACCGAA6640.13114184254288772No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6630.13094433976797373No Hit
TCCAAATTACCTCCCACCCAGGTGGCTAGATTCATCAACTCACCCCAACA6490.1281793009191779No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6430.12699428426969397No Hit
ATGTCAGGAAAAGAAGGAGTTTGCCAATTAGGATTAAAGCAAGGTACCGT6340.1252167592954681No Hit
TATACAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATAT6310.12462425097072614No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6210.12264922322158626No Hit
CAATTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAGTTGT6160.12166170934701631No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6150.12146420657210234No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC6090.1202791899226184No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6040.11929167604804848No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5920.11692164274908062No Hit
ATCTCATCGAAGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATC5850.11553912332468272No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5840.11534162054976872No Hit
GTTTCGCTCCAGACCTGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA5790.1143541066751988No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5780.11415660390028479No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCCGCTCACCATACGG5770.11395910112537082No Hit
ATATGTTCTTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA5750.11356409557554284No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5740.11336659280062884No Hit
GGGACGACCCCTGGCCCGAATGCTCCCACTCCCACCTTGTTGGCGTCCGG5730.11316909002571486No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5660.11178657060131696No Hit
ACATCGACCCGTATAAAGAATTTGGAGCTTCTACGGAGTTACTCTCTTTT5620.11099655950166101No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5620.11099655950166101No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5560.10981154285217708No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5550.10961404007726308No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5540.1094165373023491No Hit
ATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCA5540.1094165373023491No Hit
TATTAACGTTGACATAGCCGACTACTAAATCCCTGGATGCTTGATCTTCC5530.1092190345274351No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT5530.1092190345274351No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTACGGAGTTACTCTCTTTT5510.10882402897760712No Hit
GTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGTC5460.1078365151030372No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5370.10605899012881131No Hit
GGGCAAAACTCATCGGGACTGACAATTCTGTCGTGCTCTCCCGCAAGTAT5290.10447896792949941No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG5280.10428146515458542No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5280.10428146515458542No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5270.10408396237967144No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTTT5260.10388645960475744No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTTAGGAAAAGAA5220.10309644850510148No Hit
GACCTGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCAGTATGGATCGG5220.10309644850510148No Hit
GGCAGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCAC5210.10289894573018751No Hit
GTTCCGGAGACTCTAAGGCCTCCCGATACAGAGCAGAGGCAGTGTCGAGG5200.10270144295527353No Hit
GATGCAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAACCAGGAGACTA5180.10230643740544555No Hit
GGTCTATATGGAGGAGGAGTGCGAATCCACACTCCAAAGGACACCAAATA5170.10210893463053156No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5170.10210893463053156No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGAAGGGGC5140.10151642630578961No Hit
GATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCC5130.1013189235308756No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5130.1013189235308756No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG5120.10112142075596163No Hit
GTGCAATTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGAAACATAGA5110.10092391798104763No Hit
CATTATTGACAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAA5080.10033140965630567No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)