FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500120
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC31160.6230504678877069No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG23710.4740862193073662No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC22740.4546908741901943No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22600.45189154602895304No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG17830.3565144365352315No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17770.3553147244661281No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC17210.3441174118211629No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA16180.32352235463488765No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG15930.3185235543469567No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT13660.2731344477325442No Hit
ATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCCATGAA13620.27233463968647525No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT13370.26733583939854433No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13260.26513636727185474No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA12470.2493401583619931No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA11730.23454370950971767No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT10970.21934735663440777No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG10380.2075501879548908No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC10300.20595057186275295No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT10200.20395105174758058No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC9910.19815244341358074No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT9250.18495561065344318No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA9230.1845557066304087No Hit
TACTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT9130.18255618651523633No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC9130.18255618651523633No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG9120.1823562345037191No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8890.17775733823882267No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT8340.16675997760537473No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA8290.16576021754778852No Hit
GTCTAAGGGCAAATACTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA8000.15996160921378869No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7990.15976165720227145No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7850.15696232904103016No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA7780.1555626649604095No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC7730.15456290490282332No Hit
CACTAAGTATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC7700.1539630488682716No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA7680.15356314484523714No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC7620.15236343277613373No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG7600.15196352875309926No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT7420.148364392545789No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7400.14796448852275454No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA7320.14636487243061666No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT7180.14356554426937534No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT7120.14236583220027194No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG7090.14176597616572023No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG7040.14076621610813403No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT6930.13856674398144445No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG6900.13796688794689274No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC6880.13756698392385827No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6870.13736703191234104No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG6840.13676717587778933No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT6830.1365672238662721No Hit
GTCCTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT6770.1353675117971687No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC6690.13376789570503078No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT6630.13256818363592737No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT6600.13196832760137567No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT6600.13196832760137567No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT6550.1309685675437895No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6540.13076861553227226No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6470.1293689514516516No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6400.12796928737103094No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGT6380.12756938334799647No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA6280.12556986323282412No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA6270.1253699112213069No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT6230.12457010317523795No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA6050.12097096696792768No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG6040.12077101495641046No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT6030.12057106294489323No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATACTTAGTGTGGGT6020.12037111093337598No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG6010.12017115892185876No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA5950.11897144685275535No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGTCCCTTTATACC5920.11837159081820364No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT5880.11757178277213469No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAAGGGACTC5850.11697192673758298No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA5840.11677197472606575No Hit
GTCCAACTGATGATCGGGAAAGAATCCCAGAGGGTTGGGAACAGAAAGAT5770.11537231064544509No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT5730.11457250259937614No Hit
TTCCCCCACTGTTTGGCTTTCAGCTATATGGATGATGTGGTATTGGGGGC5700.11397264656482443No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTT5700.11397264656482443No Hit
GTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCC5690.11377269455330721No Hit
ATTCTATCCTACCCACACTAAGTATTTGCCCTTAGACAAAGGAATTAAAC5670.11337279053027273No Hit
ATTCTATGCTGGGGGGAATTGATGACTCTAGCTACCTGGGTGGGTAATAA5630.1125729824842038No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC5610.11217307846116932No Hit
GTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTTGC5600.1119731264496521No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG5580.1115732224266176No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG5550.1109733663920659No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC5450.10897384627689354No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCTCG5430.10857394225385908No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC5420.10837399024234183No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG5360.10717427817323842No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5340.10677437415020395No Hit
GAATACAGGTGCAATTTCCATCCGTAGGTTTTGTACAGCAACATGAGGGA5290.10577461409261776No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA5280.10557466208110054No Hit
GTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5220.1043749500119971No Hit
CAGTTGGACCCTGCATTCGGAGCCAACTCAAACAATCCAGATTGGGACTT5170.10337518995441095No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA5150.10297528593137648No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGATCTGATGGGCGTTC5100.10197552587379029No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTTCCCCAACTTC5070.10137566983923858No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG5050.10097576581620411No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC5050.10097576581620411No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5040.10077581380468688No Hit
GTACTTGAATATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5020.10037590978165241No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)