FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340305
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAA17250.506898223652312No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA17100.5024904130118569No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA16930.4974948942860081No Hit
CCTCCAGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA16570.4869161487489164No Hit
CCTCAAGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA16430.4828021921511585No Hit
CACATAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAA13070.38406723380496904No Hit
GTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA12500.3673175533712405No Hit
AAACTACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAA11230.3299980899487225No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC11110.3264718414363586No Hit
GTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG10490.30825289078914503No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAACACATAGATC10460.30737132866105404No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAA10400.30560820440487213No Hit
CATAACTGACTACTAGGTCCCTGGATGTTGGATCTTGCAAATTACCACCC10270.3017881018498112No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA9960.2926786265262044No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG9950.29238477248350747No Hit
GTCACTTATGTTAATACTAATATGGGCCTAAAGTTCAGGCAACTACTGTG9920.29150321035541643No Hit
CACAAAAATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAA9770.28709539971496156No Hit
CTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA9650.2835691512025977No Hit
GGGTTTCACCCCACCGCACGGAGGCCTTTTGGGGACGAGCCCTCAGGCTC9110.26770103289696007No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9080.26681947076886914No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT8300.2438988554385037No Hit
TATGCGTCCAAGGAACACTAACATTGAGGTTCCCGAGATTGAGATCTTCT8270.24301729331041275No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG8240.24213573118232173No Hit
GTTAACAGACCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGCCTTTT7490.22009667798004734No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7470.21950896989465335No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7330.21539501329689545No Hit
GTCACTTATGTTAATACTAATGTGGGCCTAAAGTTCAGGCAACTACTGTG7330.21539501329689545No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAACACAAAAATC7320.21510115925419843No Hit
GTGAAACCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCCA7270.21363188904071345No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA7120.20922407840025858No Hit
TTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACACT7110.20893022435756162No Hit
GAATACAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAACACTAAC7060.20746095414407661No Hit
TCTAACAACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTTGTG7030.20657939201598566No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG6970.20481626775980372No Hit
GTCTATTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTCTGTATAATG6860.2015838732901368No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC6530.19188668988113605No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC6380.18747887924068116No Hit
GGGACTCACCCCACCGCACGGAGGCCTTTTGGGGACGAGCCCTCTGGCTC6290.18483419285640823No Hit
GAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC6240.18336492264292326No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6190.18189565242943828No Hit
GTAGAAGCAGGAGGCGGATTTGCTGGCAAGGTTTGTAGTATGCCCTGAGC6120.17983867413055935No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5740.16867222050807362No Hit
CTTTAGGACATGGACATCGACCCTTATAAAGAATTTGGAGCTTCTGTGGA5720.16808451242267966No Hit
GTCCTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5590.16426440986761875No Hit
GTAGTGGCAGGAGGCGGATTTGCTGGCAAGGTTTGTAGTATGCCCTGAGC5580.16397055582492176No Hit
TAACCGACCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGCCTTTTGG5430.15956274518446686No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA5310.15603649667210298No Hit
GTAGAAGCAGGAGGCGGATTTGCTGGCAAAGTTTGTAGTATGCCCTGAGC5220.15339181028783003No Hit
CTATAAGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC5210.15309795624513303No Hit
CTGCTTCTACCAATCGCCAGTCAGGAAGGCAGCCAACCCCTCTGTCTCCA5070.14898399964737516No Hit
CAGCCGGTCTGGAGCAAACATTCTCGGGACGGATAACTCTGTTGTTCTCT5000.1469270213484962No Hit
GTTCAGCGCAAGGTCCCCAATCCTCGAGAAGATTGACGATATGTGAGAGA4990.1466331673057992No Hit
ATGTAAGACCTTGGGCAAGACTAGGTGGGCGTTCACGGTGGTCTCCATGC4970.1460454592204052No Hit
TCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA4960.14575160517770822No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT4940.14516389709231425No Hit
GGTCCTGCATGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGAC4790.14075608645185936No Hit
GGTGAGTCCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCC4700.13811140006758643No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACTAGGTGGGCGTTC4690.13781754602488944No Hit
GGTGAATCCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCC4640.13634827581140446No Hit
ACCGAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA4630.13605442176870747No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4620.1357605677260105No Hit
GGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCGGCACAAGGTCCCC4610.1354667136833135No Hit
ATACCACATCATCCATATAACTAAAAGCCAAACAGTGGGGGAAAGCCCTA4530.13311588134173755No Hit
CACATAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCCTTAA4520.13282202729904055No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4460.1310589030428586No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT4460.1310589030428586No Hit
AGTCGGAGGACAGGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAAT4460.1310589030428586No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGCGTCCAAGGAACACTAAC4380.12870807070128268No Hit
GAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACA4300.12635723835970675No Hit
CTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCAC4300.12635723835970675No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4280.12576953027431276No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4240.12459411410352476No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4150.12194942771925185No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4130.12136171963385786No Hit
AATGACTCTAGCCACCTGGGTGGGTGGTAATTTGCAAGATCCAACATCCA4090.1201863034630699No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGGCCCACAATACGTTGACAGACT4080.1198924494203729No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACT4060.11930474133497891No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT4060.11930474133497891No Hit
TATCCAAAGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATA4040.11871703324958494No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3960.11636620090800899No Hit
GCTGTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT3930.115484638779918No Hit
GTACAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3890.11430922260913005No Hit
GGTCTGTTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTCTGTATAAT3880.11401536856643305No Hit
GGGACTCACCCCACCGCACGGAGGCCTTTTGGGGACGAGCCCTCAGGCTC3830.1125460983529481No Hit
ACAACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAAA3810.11195839026755411No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA3780.11107682813946314No Hit
GTGCCGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT3770.11078297409676614No Hit
CAGCAGTAAAAAGGGACTCAAGGTGCGATACAGATTTGGCCCCCAATACC3770.11078297409676614No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG3760.11048912005406915No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT3720.10931370388328117No Hit
CAAAAATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAAC3700.10872599579788719No Hit
GGTTGTAGGAATCCTGATGTGATGTTCTCCATGTTCGGCACAAGGTCCCC3700.10872599579788719No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG3690.1084321417551902No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA3680.1081382877124932No Hit
GCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC3670.10784443366979621No Hit
GGGGACCTAATGACTCTAGCCACCTGGGTGGGTGGTAATTTGCAAGATCC3660.10755057962709921No Hit
ATACTAATGTGGGCCTAAAGTTCAGGCAACTACTGTGGTTTCACATTTCT3630.10666901749900824No Hit
CTCTACTTCCAGGATCTTCAACCACCAGCACGGGACCATGCAGAACCTGC3620.10637516345631125No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG3610.10608130941361425No Hit
GACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA3610.10608130941361425No Hit
ACGTACAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCAC3510.10314276898664435No Hit
GTATGGATCGGCAGTGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC3460.10167349877315938No Hit
TTCCCCCACTGTTTGGCTTTTAGTTATATGGATGATGTGGTATTGGGGGC3450.10137964473046239No Hit
GAATACAGCCCCGTAAAGTTTCCCACCTTATGCGTCCAAGGAACACTAAC3440.1010857906877654No Hit
GTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCGAG3420.1004980826023714No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA3420.1004980826023714No Hit
GGTGAAACCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCC3410.1002042285596744No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT3410.1002042285596744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)