FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340305
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA16270.47810052746800663No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA16020.47075417640058187No Hit
AAACAACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAA15370.4516536636252773No Hit
CCTCCAGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA14700.4319654427645789No Hit
CCTCAAGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA14330.4210928431847901No Hit
GTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA12190.3582080780476337No Hit
AAACTACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAA10730.31530538781387285No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC9890.2906216482273255No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG9750.2865076916295676No Hit
CTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA9610.2823937350318097No Hit
GTCACTTATGTTAATACTAATATGGGCCTAAAGTTCAGGCAACTACTGTG9260.27210884353741494No Hit
CACATAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAA9100.26740717885426313No Hit
GTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG9080.26681947076886914No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9070.2665256167261721No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA8770.25770999544526235No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAACACATAGATC8600.2527144767194135No Hit
CATAACTGACTACTAGGTCCCTGGATGTTGGATCTTGCAAATTACCACCC8530.25065749842053453No Hit
GGGTTTCACCCCACCGCACGGAGGCCTTTTGGGGACGAGCCCTCAGGCTC8270.24301729331041275No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAA8050.2365525043710789No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7910.23243854777332099No Hit
TATGCGTCCAAGGAACACTAACATTGAGGTTCCCGAGATTGAGATCTTCT7620.22391678053510822No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT7520.2209782401081383No Hit
GTTAACAGACCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGCCTTTT7370.21657042946768343No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA7210.21186876478453154No Hit
GTCACTTATGTTAATACTAATGTGGGCCTAAAGTTCAGGCAACTACTGTG6860.2015838732901368No Hit
CTTTAGGACATGGACATCGACCCTTATAAAGAATTTGGAGCTTCTGTGGA6800.1998207490339548No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6750.19835147882046986No Hit
CACAAAAATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAA6740.1980576247777729No Hit
GTCTATTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTCTGTATAATG6660.19570679243619696No Hit
TTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACACT6580.193355960094621No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6510.19129898179574203No Hit
GTGAAACCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCCA6410.18836044136877214No Hit
GAATACAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAACACTAAC6390.18777273328337815No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC6380.18747887924068116No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAACACAAAAATC6120.17983867413055935No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC6090.1789571120024684No Hit
TCTAACAACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTTGTG6020.17690013370358942No Hit
GAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC6000.17631242561819543No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG5910.1736677392339225No Hit
GGGACTCACCCCACCGCACGGAGGCCTTTTGGGGACGAGCCCTCTGGCTC5590.16426440986761875No Hit
CAGCCGGTCTGGAGCAAACATTCTCGGGACGGATAACTCTGTTGTTCTCT5550.16308899369683078No Hit
GTAGAAGCAGGAGGCGGATTTGCTGGCAAGGTTTGTAGTATGCCCTGAGC5520.16220743156873982No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA5500.16161972348334583No Hit
CTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCAC5320.15633035071479998No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5270.154861080501315No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT5000.1469270213484962No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG4970.1460454592204052No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG4800.14104994049455635No Hit
GTAGAAGCAGGAGGCGGATTTGCTGGCAAAGTTTGTAGTATGCCCTGAGC4570.13429129751252553No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4550.13370358942713156No Hit
GGTCCTGCATGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGAC4520.13282202729904055No Hit
TATCCAAAGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATA4510.13252817325634356No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4470.1313527570855556No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGCGTCCAAGGAACACTAAC4430.13017734091476765No Hit
GTCCTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4360.1281203626158887No Hit
TAACCGACCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGCCTTTTGG4360.1281203626158887No Hit
CTGCCACTACCAATCGCCAGTCAGGAAGGCAGCCAACCCCTCTGTCTCCA4350.1278265085731917No Hit
ACCGAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA4310.12665109240240371No Hit
GTTCAGCGCAAGGTCCCCAATCCTCGAGAAGATTGACGATATGTGAGAGA4250.12488796814622176No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT4230.1243002600608278No Hit
GGTGAATCCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCC4220.1240064060181308No Hit
CTATAAGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC4200.12341869793273681No Hit
AGTCGGAGGACAGGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAAT4120.12106786559116087No Hit
ATACCACATCATCCATATAACTAAAAGCCAAACAGTGGGGGAAAGCCCTA4070.1195985953776759No Hit
CTGCTTCTACCAATCGCCAGTCAGGAAGGCAGCCAACCCCTCTGTCTCCA4060.11930474133497891No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA3980.11695390899340297No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGGCCCACAATACGTTGACAGACT3950.116072346865312No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA3920.11519078473722101No Hit
CTCTATGACCACTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA3900.11460307665182705No Hit
GGTGAGTCCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCC3870.11372151452373605No Hit
GAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACA3800.11166453622485711No Hit
GGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCGGCACAAGGTCCCC3780.11107682813946314No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG3780.11107682813946314No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACTAGGTGGGCGTTC3770.11078297409676614No Hit
GCTGTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT3760.11048912005406915No Hit
GTGCCGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT3750.11019526601137215No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT3710.10901984984058419No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3640.10696287154170524No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3610.10608130941361425No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT3600.10578745537091726No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC3600.10578745537091726No Hit
TTGCTCACCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG3580.10519974728552328No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA3580.10519974728552328No Hit
ATACTAATGTGGGCCTAAAGTTCAGGCAACTACTGTGGTTTCACATTTCT3560.10461203920012929No Hit
GGTTGTAGGAATCCTGATGTGATGTTCTCCATGTTCGGCACAAGGTCCCC3560.10461203920012929No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT3550.1043181851574323No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG3540.1040243311147353No Hit
AATGACTCTAGCCACCTGGGTGGGTGGTAATTTGCAAGATCCAACATCCA3530.10373047707203831No Hit
TTCCCCCACTGTTTGGCTTTTAGTTATATGGATGATGTGGTATTGGGGGC3530.10373047707203831No Hit
CAGCAGTAAAAAGGGACTCAAGGTGCGATACAGATTTGGCCCCCAATACC3460.10167349877315938No Hit
ATGTAAGACCTTGGGCAAGACTAGGTGGGCGTTCACGGTGGTCTCCATGC3460.10167349877315938No Hit
GGTGAAACCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCC3450.10137964473046239No Hit
CATTTAAACACTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT3440.1010857906877654No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)