FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378851
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC20380.5379423572855819No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGAAAGTAATGATTAACTAAA19710.5202573043228077No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17080.4508368725435593No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC15850.4183702827760782No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG15160.4001573177845644No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9940.2623722782835468No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9620.25392568582371433No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT9560.25234194973749574No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC9260.2444232693064028No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG8780.23175338061665404No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTGTCCACCAAACTCTGCAAG8180.21591601975446809No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT8020.21169272352455187No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAACGTGAAACCACA7820.20641360323715657No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT7810.20614964722278678No Hit
GTTGTGGAGTTCCACTGCATGGCCTGAGGATGAGTGTTTCTCAAAGGTGG7760.20482986715093798No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7420.19585536266236592No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA7390.19506349461925665No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7330.19347975853303806No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7220.19057624237497064No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7190.18978437433186135No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7130.18820063824564276No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG7100.18740877020253346No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7090.18714481418816367No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC6990.18450525404446605No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT6960.18371338600135675No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6750.17817030969959166No Hit
CTCTTGTACATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC6420.1694597612253894No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6420.1694597612253894No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT6300.1662922890529522No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6170.16286086086614526No Hit
GTATGGATCGGCAGAGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC6140.16206899282303597No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC6120.16154108079429644No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATGCTGGATCTTC6060.15995734470807785No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6060.15995734470807785No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACC6000.15837360862185926No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT5900.1557340484781616No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC5900.1557340484781616No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5890.15547009246379184No Hit
GCCTAATCATCTCTTGTACATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5700.1504549281907663No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG5590.14755141203269886No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5470.14438393986026168No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5450.14385602783152215No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG5450.14385602783152215No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC5430.14332811580278262No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5430.14332811580278262No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATC5310.14016064363034544No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTTCAAACC5280.13936877558723615No Hit
CTCCACAACTGTCCACCAAACTCTGCAAGATCCCAGGGTGAGAGGCCTGT5230.13804899551538732No Hit
CCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTATGCCAAGTGTTTGCTG5220.13778503950101756No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5210.13752108348664777No Hit
GTGTAAATAATGTCTGGTTTGAAAGTAATGATTAACTAAATGTTCCGGAT5190.13699317145790826No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5150.13593734740042918No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAACCCCTGTTCCGA5080.13408965529984085No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5050.13329778725673155No Hit
CACTAATATGGGCCTAAAGTTCAGACAATTATTGTGGTTTCACGTTTCTT4970.1311861391417734No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT4870.12854657899807576No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4850.12801866696933623No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT4800.1266988868974874No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT4770.1259070188543781No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGT4760.12564306284000834No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4690.12379537073941999No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG4670.12326745871068046No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATG4670.12326745871068046No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4660.12300350269631069No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4630.1222116346532014No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4520.11930811849513398No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4510.1190441624807642No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4500.11878020646639442No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4490.11851625045202469No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA4480.1182522944376549No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4470.11798833842328516No Hit
AGTCTGTACAGCATCTTGAGGCCCTTTTTACCGCTGTTACCAATTTTCTT4430.11693251436580608No Hit
GTACATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG4410.11640460233706657No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA4370.11534877827958749No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTGTTTGATTTTCTGT4310.1137650421933689No Hit
GCGCGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG4260.11244526212152008No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4230.11165339407841078No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT4220.111389438064041No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT4220.111389438064041No Hit
TCTCGGGGCCGCTTGGGGGTCTTTCGTCCCCTTCTCCGTCTGCCGTTTCG4210.11112548204967124No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4180.11033361400656194No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG4140.1092777899490829No Hit
ATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGTGG4140.1092777899490829No Hit
CTTGTACATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT4050.106902185819755No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4040.10663822980538523No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4040.10663822980538523No Hit
CCCTGGGATCTTGCAGAGTTTGGTGGACAGTTGTGGAGTTCCACTGCATG4030.10637427379101548No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA4030.10637427379101548No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGGCCCTTTTTACC4030.10637427379101548No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA4020.1061103177766457No Hit
CCCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCGAGGATT3950.10426262567605733No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATTCAATCTAAGC3930.10373471364731782No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC3920.10347075763294804No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG3920.10347075763294804No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA3920.10347075763294804No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG3910.10320680161857829No Hit
GCTTTAATGCCCTTGTATGCCTGTATTCAATCTAAGCAGGCTTTCACTTT3890.10267888958983874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)