FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169458
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9710.573003340060664No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC5320.3139420977469343No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5030.2968287127193759No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4260.25138972488758277No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA4180.24666879108687698No Hit
ATATAAGACCTTGGGCAAGAGTTGGCGGGCGTTCAAGGTGGTCTCCATGC4070.24017750711090655No Hit
CCTACAGCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA3990.23545657331020078No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT3220.19001758547840764No Hit
CTATGAATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3180.18765711857805475No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACATAGCGCC3140.18529665167770185No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG3000.17703501752646672No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACCTTCCACCAAACTCCTGCTG2810.1658227997497905No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT2800.1652326830247023No Hit
ACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGATCT2760.1628722161243494No Hit
CTACAAACTTTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC2690.15874139904873186No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT2650.15638093214837895No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTCCTAAT2530.14929953144732028No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTCCTGGAT2360.13926754712082048No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC2290.13513673004520294No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGATTAAAGA2290.13513673004520294No Hit
CTACACACTTTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC2290.13513673004520294No Hit
AGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT2250.13277626314485005No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTGCTCCCTAAATTTTAT2180.1286454460692325No Hit
ATCACGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC2170.12805532934414426No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA2160.12746521261905605No Hit
CTATTTACACACTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAA2140.1262849791688796No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT2130.12569486244379138No Hit
GTTCAGCGCAGGGTCCCCAATCCTCGAGAAGATTGACGATAAGTGAGAGG2120.12510474571870317No Hit
GCTGTAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAAAAC2000.11802334501764448No Hit
ATCTCTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGGAT1990.11743322829255627No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC1970.11625299484237983No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG1960.11566287811729162No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC1940.11448264466711515No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTCTGATTTTTTGT1930.11389252794202694No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT1930.11389252794202694No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA1930.11389252794202694No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG1910.1127122944918505No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT1900.11212217776676227No Hit
GGATAAGGGTATTAAACCTTATTATCCAGGACATCTAGTTAATCATTACT1900.11212217776676227No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC1840.10858147741623293No Hit
TACCGTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG1830.10799136069114472No Hit
GAGTAAAAGGGGCAGCAAAACCCAAAAGACCCGCAATTCGTTGACAGACT1810.10681112724096825No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT1800.10622101051588004No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG1790.10563089379079181No Hit
GCCTGAGGATGTGTGTCTCTCAAAGGTGGAGACAACGGGGTAGGCTGCCT1780.1050407770657036No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA1770.10445066034061537No Hit
GTTTGTAGGCCCACTCACAGTTAACGAGAAAAGAAGATTGCAATTGATCA1740.1026803101653507No Hit
ACACTAATGTGGGCCTAAAGTTCAGGCAGCTCTTGTGGTTTCACATTTCT1730.10209019344026247No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGAATTGCGGGTCTTTTGGGTTTT1710.10090995999008603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)