FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397226
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA22530.5671834169968734No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC19920.5014777481836536No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18030.4538977811120118No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC15970.4020381344624974No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG12540.3156893053324808No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11640.29303217815550847No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11070.2786826642767593No Hit
ATACAAACCTTACCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC10960.27591345984401827No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC10570.26609537140066364No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA9360.23563412264051195No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9260.23311666406529283No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT9220.23210968063520515No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC8380.21096302860336433No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT8110.2041658904502726No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7960.2003897025874439No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATATGAAACCACA7930.19963446501487817No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG7650.19258558100426457No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT7560.19031986828656733No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG7480.18830590142639203No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7470.18805415556887012No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7410.18654368042373864No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT7200.18125701741577843No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTACATTTCAT7170.18050177984321267No Hit
GCCTAATCATCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7140.17974654227064693No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7000.17622210026534013No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6910.1739563875476429No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA6770.1704319455423361No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6700.1686697245396827No Hit
ATCCAACATCTAGGGACCTAGTAGTCAGCTATGTCAACACTAATATGGGC6600.16615226596446356No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG6570.16539702839189782No Hit
GGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAGACGGGGA6480.16313131567420058No Hit
CCCTTATCTAATGGCAAATATTTAGTAACCTTTGGATAAAACCTAGCAGG6470.16287956981667867No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6390.16086560295650335No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6200.15608243166358698No Hit
ATATTAGTGTTGACATAGCTGACTACTAGGTCCCTAGATGTTGGATCTTC6130.15432021066093357No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCATATTTCTT6080.153061481373324No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6030.15180275208571442No Hit
CTTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT6010.1512992603706706No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTAGTAACCTTTGGATAAAAC6000.15104751451314868No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT5970.15029227694058295No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5880.1480265642228857No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC5840.14701958079279806No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5710.14374688464501317No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5600.1409776802122721No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC5530.1392154592096187No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5510.13871196749457487No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5500.13846022163705296No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5500.13846022163705296No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5490.13820847577953105No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT5460.13745323820696528No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5400.1359427630618338No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5350.13468403377422422No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT5340.13443228791670234No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT5310.13367705034413657No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAGATACAGGCCTCT5290.13317355862909275No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5280.13292181277157086No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT5270.13267006691404892No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5120.1288938790512202No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT5110.1286421331936983No Hit
CCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5010.12612467461847915No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTGAAACATTCTTTGATTTTCTGT5000.12587292876095724No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4990.12562118290343532No Hit
ATCATCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGG4950.12461419947334766No Hit
GTAGAGGAATAAAGCCCCGTAAAGTTACCCACTTTATGAGTCCAAGGAAT4940.12436245361582576No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4890.12310372432821617No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG4880.12285197847069426No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC4870.12260023261317235No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA4800.12083801161051895No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA4780.12033451989547513No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA4750.11957928232290939No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4740.11932753646538745No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4700.1183205530352998No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4690.11806880717777789No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCC4630.11655833203264641No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACACACGGGCAAC4610.11605484031760258No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA4550.11454436517247107No Hit
GAGTAACCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGA4470.11253039831229576No Hit
ATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGTGG4390.11051643145212045No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT4350.10950944802203279No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC4320.10875421044946705No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4320.10875421044946705No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4300.10825071873442323No Hit
GTACAGTAGAGGAATAAAGCCCCGTAAAGTTACCCACTTTATGAGTCCAA4300.10825071873442323No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTT4240.10674024358929173No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG4200.10573326015920408No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA4190.10548151430168216No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4120.10371929329902876No Hit
GTCCAGTAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4080.1027123098689411No Hit
GCATAATACAAACCTTACCAGCAAATCCGCCTCCTGCATCTACCAATCGC4050.10195707229637535No Hit
CTATTGTGGTTTCATATTTCTTGTCTCACTTTTGGAAGAGAAACAGTCAT4040.10170532643885345No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG4030.10145358058133154No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA4020.10120183472380963No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)