FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397226
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA21780.5483024776827298No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC18750.47202348285358964No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17470.43980001309078465No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC14980.3771152945678279No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG13800.34740928338024196No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11420.28749376929002635No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10390.2615639459652691No Hit
ATACAAACCTTACCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC9940.25023538237678294No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT8830.2222915921918505No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT8640.2175084208989341No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT8070.203158907020185No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA8010.2016484318750535No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7940.19988621087240008No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7920.19938271915735628No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7770.19560653129452754No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATATGAAACCACA7770.19560653129452754No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7600.191326851716655No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC7520.18931288485647968No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTAGTAACCTTTGGATAAAAC7450.1875506638538263No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG7410.18654368042373864No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTACATTTCAT7340.18478145942108523No Hit
GCCTAATCATCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7320.18427796770604138No Hit
CCCTTATCTAATGGCAAATATTTAGTAACCTTTGGATAAAACCTAGCAGG7320.18427796770604138No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7250.182515746703388No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG7190.18100527155825652No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7170.18050177984321267No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6650.16741099525207315No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA6580.16564877424941973No Hit
ATATTAGTGTTGACATAGCTGACTACTAGGTCCCTAGATGTTGGATCTTC6560.16514528253437588No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6160.15507544823349934No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5980.15054402279810486No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT5960.150040531083061No Hit
ATCCAACATCTAGGGACCTAGTAGTCAGCTATGTCAACACTAATATGGGC5930.1492852935104953No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT5900.14853005593792953No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC5840.14701958079279806No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5820.14651608907775424No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5720.14399863050253509No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCATATTTCTT5630.14173291778483785No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC5620.14148117192731594No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5620.14148117192731594No Hit
CTTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT5600.1409776802122721No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5560.13997069678218446No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5490.13820847577953105No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5470.13770498406448722No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG5430.13669800063439957No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5350.13468403377422422No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5280.13292181277157086No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT5250.1321665751990051No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5240.1319148293414832No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT5230.13166308348396127No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT5170.1301526083388298No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAGATACAGGCCTCT5170.1301526083388298No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5160.12990086248130786No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG5010.12612467461847915No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5010.12612467461847915No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC4990.12562118290343532No Hit
CCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4970.12511769118839147No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTGAAACATTCTTTGATTTTCTGT4920.12385896190078192No Hit
ATCATCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGG4870.12260023261317235No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4840.1218449950406066No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG4810.12108975746804086No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4800.12083801161051895No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4800.12083801161051895No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG4760.11983102818043129No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT4760.11983102818043129No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTAGTAACCTTTGGATAAAA4760.11983102818043129No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC4740.11932753646538745No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA4700.1183205530352998No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG4700.1183205530352998No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4700.1183205530352998No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4600.11580309446008066No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT4540.11429261931494916No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4540.11429261931494916No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACACACGGGCAAC4540.11429261931494916No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4520.11378912759990535No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCC4510.11353738174238343No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA4480.11278214416981769No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT4480.11278214416981769No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT4430.1115234148822081No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA4420.1112716690246862No Hit
GGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAGACGGGGA4400.11076817730964238No Hit
GAGTAACCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGA4400.11076817730964238No Hit
GTACAGTAGAGGAATAAAGCCCCGTAAAGTTACCCACTTTATGAGTCCAA4380.11026468559459854No Hit
ATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGTGG4320.10875421044946705No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4250.10699198944681367No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC4240.10674024358929173No Hit
GACCTGGCCGTTGCCTAGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4220.10623675187424791No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4200.10573326015920408No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC4180.10522976844416025No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT4180.10522976844416025No Hit
GTAGAGGAATAAAGCCCCGTAAAGTTACCCACTTTATGAGTCCAAGGAAT4120.10371929329902876No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT4090.10296405572646301No Hit
GCTTTAATGCCCTTGTATGCCTGTATTCAATCTAAACAGGCTTTCACTTT4080.1027123098689411No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4070.10246056401141919No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4020.10120183472380963No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA4010.1009500888662877No Hit
TCTCGGGGCCGCTTGGGGATCTTTCGTCCCCTTCTCCGTCTGCCGTTTCG3980.10019485129372195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)